Spb4p, an essential putative RNA helicase, is required for a late step in the assembly of 60S ribosomal subunits in Saccharomyces cerevisiae

Cruz, Jesús de la; Kressler, Dieter; Rojo, Manuel; Tollervey, David; Linder, Patrick

doi:10.1017/s1355838298981158

Cited by 82 publications

(32 citation statements)

References 45 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Sucrose gradients were analysed using an ISCO UA‐6 system with continuous monitoring at A 254 . When required, fractions of 0.5 mL were collected from the gradients and proteins were extracted and analysed by western blotting as described .…”

Section: Methodsmentioning

confidence: 99%

Role of the yeast ribosomal protein L16 in ribosome biogenesis

et al. 2016

View full text Add to dashboard Cite

Most ribosomal proteins play essential roles in ribosome synthesis and function. In this study, we have analysed the contribution of yeast ribosomal protein L16 to ribosome biogenesis. We show that in vivo depletion of the essential L16 protein results in a deficit in 60S subunits and the appearance of half-mer polysomes. This phenotype is likely due to the instability and rapid turnover of early and intermediate pre-60S particles, as evidenced by the reduced steady-state levels of 27SB S and 7S L/S pre-rRNA, and the low amounts of de novo synthesized 27S pre-rRNA and 25S rRNA. Additionally, depletion of L16 blocks nucleocytoplasmic export of pre-60S particles. Moreover, we show that L16 assembles in the nucleolus and binds to early 90S preribosomal particles. Many evolutionarily conserved ribosomal proteins possess extra eukaryote-specific amino-or carboxy-terminal extensions and/or internal loops. Here, we have also investigated the role of the eukaryote-specific carboxy-terminal extension of L16. Progressive truncation of this extension recapitulates, albeit to a lesser extent, the growth and ribosome biogenesis defects of the L16 depletion. We conclude that L16 assembly is a prerequisite to properly stabilize rRNA structures within early pre-60S particles, thereby favouring efficient 27S pre-rRNA processing within the internal transcribed spacer 1 at sites A 3 and B 1 . Upon depletion of L16, the lack of this stabilization aborts early pre-60S particle assembly and subjects these intermediates to turnover.

show abstract

Section: Methodsmentioning

confidence: 99%

Role of the yeast ribosomal protein L16 in ribosome biogenesis

et al. 2016

View full text Add to dashboard Cite

show abstract

“…When needed, fractions of 0.5 ml were collected from the gradients; protein and RNA were extracted from the different fractions as exactly described [70], and analysed as described below by northern or western blot analyses.…”

Section: Methodsmentioning

confidence: 99%

Final Pre-40S Maturation Depends on the Functional Integrity of the 60S Subunit Ribosomal Protein L3

et al. 2014

Self Cite

View full text Add to dashboard Cite

Ribosomal protein L3 is an evolutionarily conserved protein that participates in the assembly of early pre-60S particles. We report that the rpl3[W255C] allele, which affects the affinity and function of translation elongation factors, impairs cytoplasmic maturation of 20S pre-rRNA. This was not seen for other mutations in or depletion of L3 or other 60S ribosomal proteins. Surprisingly, pre-40S particles containing 20S pre-rRNA form translation-competent 80S ribosomes, and translation inhibition partially suppresses 20S pre-rRNA accumulation. The GTP-dependent translation initiation factor Fun12 (yeast eIF5B) shows similar in vivo binding to ribosomal particles from wild-type and rpl3[W255C] cells. However, the GTPase activity of eIF5B failed to stimulate processing of 20S pre-rRNA when assayed with ribosomal particles purified from rpl3[W255C] cells. We conclude that L3 plays an important role in the function of eIF5B in stimulating 3′ end processing of 18S rRNA in the context of 80S ribosomes that have not yet engaged in translation. These findings indicate that the correct conformation of the GTPase activation region is assessed in a quality control step during maturation of cytoplasmic pre-ribosomal particles.

show abstract

“…It was further demonstrated that in one of the loci, the RNA HELICASE 18 ( RH18 ) gene was required for the observed hybrid chlorosis phenotype. The function of RH18 is still uncharacterized in plants, but its homolog in yeast is known to be involved in 25S rRNA precursor processing (Cruz et al ., ). These studies suggest that in addition to rapidly evolving genes, highly conserved genes can also contribute to hybrid incompatibilities.…”

Section: Hybrid Incompatibilities Caused By Interactions Between Genementioning

confidence: 97%

Diverse paths to hybrid incompatibility in Arabidopsis

Vaid

Laitinen

2018

The Plant Journal

View full text Add to dashboard Cite

Summary One of the most essential questions of biology is to understand how different species have evolved. Hybrid incompatibility, a phenomenon in which hybrids show reduced fitness in comparison with their parents, can result in reproductive isolation and speciation. Therefore, studying hybrid incompatibility provides an entry point in understanding speciation. Hybrid incompatibilities are known throughout taxa, and the underlying mechanisms have mystified scientists since the theory of evolution by means of natural selection was introduced. In plants, it is only in recent years that the high‐throughput genetic and molecular tools have become available for the Arabidopsis genus, thus helping to shed light on the different genes and molecular and evolutionary mechanisms that underlie hybrid incompatibilities. In this review, we highlight the current knowledge of diverse mechanisms that are known to contribute to hybrid incompatibility.

show abstract

Spb4p, an essential putative RNA helicase, is required for a late step in the assembly of 60S ribosomal subunits in Saccharomyces cerevisiae

Cited by 82 publications

References 45 publications

Role of the yeast ribosomal protein L16 in ribosome biogenesis

Role of the yeast ribosomal protein L16 in ribosome biogenesis

Final Pre-40S Maturation Depends on the Functional Integrity of the 60S Subunit Ribosomal Protein L3

Diverse paths to hybrid incompatibility in Arabidopsis

Contact Info

Product

Resources

About