Spatiotemporal Regulators for Insulin-Stimulated GLUT4 Vesicle Exocytosis

Zhou, Xiaoxu; Shentu, Ping; Xu, Yingke

doi:10.1155/2017/1683678

Cited by 20 publications

(18 citation statements)

References 114 publications

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“…In this study, we investigated whether there is a synergistic effect of lithium and insulin combination treatment on internalization rate of GLUT4myc; no synergistic effect was observed. In the basal state, approximately 10% of the GLUT4 is located at the cell surface and 90% in intracellular membrane compartments (33). However, in this study, the internalization rate of GLUT4myc at basal level was about 45%, The cause of this result is unclear, but is thought to be due to the specificity of the L6-GLUT4myc stable cell line.…”

Section: Glut4myc Internalizationcontrasting

confidence: 64%

Effect of Lithium on the Mechanism of Glucose Transport in Skeletal Muscles

Jung

Koh

Kim

et al. 2017

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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Summary While lithium is known to stimulate glucose transport into skeletal muscle, the mechanisms of the increased glucose transport by lithium in skeletal muscle are not well defined yet. We excised epitrochlearis muscles from male Wistar rats and measured the transport rates of a glucose analog into lithium-, insulin-, and muscular contraction-stimulated skeletal muscle tissue and we also analyzed the levels of cell surface glucose transport 4 using a photolabeling and multicolor immunofluorescence method. In addition, we generated a cell line that stably expresses myc-tagged GLUT4 to measure the rates of GLUT4 internalization and externalization. Lithium significantly increased 2-DG glucose transport rate in skeletal muscles; however, it was significantly lower than the stimulation induced by the maximum concentration of insulin or tetanic contraction. But co-treatment of lithium with insulin or tetanic contraction increased glucose transport rate by ~200% more than lithium alone. When skeletal muscle tissues were treated with lithium, insulin, and muscular contraction, the levels of cell surface GLUT4 protein contents were increased similarly by ~6-fold compared with the basal levels. When insulin or lithium stimuli were maintained, the rate of GLUT4myc internalization was significantly lower, and lithium was found to suppress the internalization of GLUT4myc more strongly. The lithium-induced increase in glucose uptake of skeletal muscles appears to increase in cell surface GLUT4 levels caused by decreased internalization of GLUT4. It is concluded that co-treatment of lithium with insulin and muscular contraction had a synergistic effect on glucose transport rate in skeletal muscle.

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Section: Glut4myc Internalizationcontrasting

confidence: 64%

Effect of Lithium on the Mechanism of Glucose Transport in Skeletal Muscles

Jung

Koh

Kim

et al. 2017

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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“…Moreover, the distribution of the compartment and trafficking from the compartment are insensitive to Brefeldin A. These observations are reminiscent of GLUT4 storage vesicles (GSVs) that are specialized for the storage and resurfacing of GLUT4 in specific cell types, notably myocytes and adipocytes (Jaldin-Fincati et al, 2017; Zhou et al, 2017). GSVs are positive for syntaxin-6 (Foley and Klip, 2014; Shewan et al, 2003) and are 50–80 nm in diameter, and their sequestration in a juxtanuclear compartment is insensitive to Brefeldin A (Bao et al, 1995; Chakrabarti et al, 1994; Martin et al, 2000).…”

Section: Resultsmentioning

confidence: 99%

Regulated resurfacing of a somatostatin receptor storage compartment fine-tunes pituitary secretion

Alshafie

Francis

Bednarz

et al. 2019

Journal of Cell Biology

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The surfacing of the glucose transporter GLUT4 driven by insulin receptor activation provides the prototypic example of a homeostasis response dependent on mobilization of an intracellular storage compartment. Here, we generalize this concept to a G protein–coupled receptor, somatostatin receptor subtype 2 (SSTR2), in pituitary cells. Following internalization in corticotropes, SSTR2 moves to a juxtanuclear syntaxin-6–positive compartment, where it remains until the corticotropes are stimulated with corticotropin releasing factor (CRF), whereupon SSTR2 exits the compartment on syntaxin-6–positive vesicular/tubular carriers that depend on Rab10 for their fusion with the plasma membrane. As SSTR2 activation antagonizes CRF-mediated hormone release, this storage/resurfacing mechanism may allow for a physiological homeostatic feedback system. In fact, we find that SSTR2 moves from an intracellular compartment to the cell surface in pituitary gland somatotropes, concomitant with increasing levels of serum growth hormone (GH) during natural GH cycles. Our data thus provide a mechanism by which signaling-mediated plasma membrane resurfacing of SSTR2 can fine-tune pituitary hormone release.

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“…This is evidenced by research stating GLUT-4 gene is only expressed in adult fat cells. 41,42 The limitations of the present study were the in vivo study had not yet been operated. For this reason, an in vivo study needs to be carried out for further research.…”

Section: Role Of (+)-Catechin and Proanthocyanidin Fraction In Ppar γmentioning

confidence: 92%

(+)-Catechin & Proanthocyanidin Fraction of Uncaria gambir Roxb. Improve Adipocytes Differentiation & Glucose Uptake of 3T3-L1 Cells Via Sirtuin-1, Peroxisome Proliferator-Activated Receptor γ (PPAR γ), Glucose Transporter Type 4 (GLUT-4) Expressions

et al. 2020

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Purpose : To improve adipocytes differentiation & glucose uptake activity of 3T3-L1 cells through sirtuin-1, peroxisome proliferator-activated receptor γ (PPAR γ), glucose transporter type 4 (GLUT-4) of (+)-catechin & proanthocyanidin fraction Uncaria gambir Roxb. Methods: Adipocytes differentiation activity of (+)-Catechin of Uncaria gambir Roxb. was determined by oil red O staining method & glucose uptake activity was determined by measuring 2-deoxyglucose uptake on 3T3-L1 cells. The ability of (+) - catechin as an activator of sirtuin-1 was assessed by administration of (+) - catechin with the presence of a specific inhibitor of sirtuin-1, nicotinamide. Metformin 1 mM & 5 mM were used as positive control. Sirtuin-1, PPAR γ & GLUT-4 expressions were determined by RT-PCR. Results: (+)-Catechin & proanthocyanidin fraction of Uncaria gambir Roxb. were found to increase adipocyte differentiation & glucose uptake by increasing activity of sirtuin-1 as well as metformin (P≤0.05). PPAR γ, GLUT-4 and sirtuin-1 expressions were known to be responsible for this activities. Conclusion: These results indicate that (+)–catechin & proanthocyanidin fraction of Uncaria gambir Roxb. could be utilized as a renewable bioresource to develop potential antidiabetic and antiobesity agents.

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Spatiotemporal Regulators for Insulin-Stimulated GLUT4 Vesicle Exocytosis

Cited by 20 publications

References 114 publications

Effect of Lithium on the Mechanism of Glucose Transport in Skeletal Muscles

Effect of Lithium on the Mechanism of Glucose Transport in Skeletal Muscles

Regulated resurfacing of a somatostatin receptor storage compartment fine-tunes pituitary secretion

(+)-Catechin & Proanthocyanidin Fraction of Uncaria gambir Roxb. Improve Adipocytes Differentiation & Glucose Uptake of 3T3-L1 Cells Via Sirtuin-1, Peroxisome Proliferator-Activated Receptor γ (PPAR γ), Glucose Transporter Type 4 (GLUT-4) Expressions

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