Abstract:AMP activated protein kinase (AMPK) is a heterotrimeric kinase essential to the maintenance of cellular energy homeostasis. AMPK has downstream substrates throughout the cell, and spatially compartmentalized AMPK activity is hypothesized to confer specificity to AMPK signaling. However, the specific spatial organization of AMPK activity and the underlying regulatory mechanisms are not clear. To monitor the compartmentalized activity of AMPK, we developed a single‐fluorophore excitation‐ratiometric AMPK activit… Show more
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