Desert farmland provides food for desert areas, but water is the main limiting factor of this region, thus desert farmland has an extremely fragile ecological environment. This study investigated the temporal and spatial variations of vegetation NDVI (Normalized Difference Vegetation Index) in the Ulan Buh Desert, China, from 1990 to 2022, using long-term Landsat satellite data obtained from the Google Earth Engine platform and local statistical data. The results showed that from 1990 to 2022, the NDVI exhibited relatively small fluctuations and a steady increase. Furthermore, the study analyzed the impact of climate factors, namely precipitation and temperature, on NDVI, and collected the groundwater lever changes under irrigation and farmland development. The results demonstrated a positive correlation between NDVI and both precipitation and temperature from 1990 to 2006. The study area experienced an overall trend of increasing humidity. Specifically, from 1990 to 2006, significant positive correlations with precipitation and temperature were observed in 4.4% and 5.5% of the region, respectively. From 2007 to 2022, significant positive correlations were observed in 5.4% and 72.8% of the region for precipitation and temperature, respectively. These findings suggest that temperature has become increasingly influential on vegetation NDVI, while the impact of precipitation remains relatively stable. Moreover, the study assessed the impact of human activities on vegetation NDVI. The results revealed that from 1990 to 2006, human activities contributed to 43.1% of the promotion of local vegetation NDVI, which increased to 90.9% from 2007 to 2022. This study provides valuable insights into the dynamics of vegetation in the Ulan Buh Desert and its response to climatic changes and human activities. The findings highlight the significance of climate conditions and human interventions in shaping the vegetation dynamics in the region, offering essential information for ecological restoration and conservation efforts.