Spatiotemporal dynamics of <i>Citrus tristeza virus</i> in Cuba

Batista, L.; Velázquez, Karelia; Estévez, Ivania; Peña, I.; Lopez, Dionisio Cordova; Reyes, Maria; Rodríguez, D.; Laranjeira, F. F.

doi:10.1111/j.1365-3059.2007.01818.x

Cited by 5 publications

(11 citation statements)

References 15 publications

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“…It was not possible to fit any progress model to the CSD incidence data (low R² and existence of residual patterns, data not shown). Conversely, tristeza progress in Cuba was best described by a logistic model for most areas (Batista et al, 2008). Nevertheless, CT progress in two Cuban fields could not be represented by any model ( Figure 1D).…”

Section: Resultsmentioning

confidence: 99%

“…A model with a good R 2 was accepted as appropriate only if its residuals showed no detectable patterns. Results for CT have been described elsewhere (Batista et al, 2008).…”

Section: Methodsmentioning

confidence: 99%

“…In each field, all trees included in a 400-plant section were sampled: 20 plants within rows x 20 plants across rows for all fields except HVN02 which had 16x25 and SC01 which had 17x24. Virus presence was determined by double antibody sandwich indirect (DASI) enzyme linked immunosorbent assay (ELISA) (Batista et al, 2008). CSD in Brazil.…”

Section: Methodsmentioning

confidence: 99%

“…Aggregation of infected plants was found in the second and third level of spatial hierarchy (Batista et al, 2008). For CSD, a preliminary epidemiological study was carried out and the results were compared to previously described tristeza epidemics in the Caribbean Basin (Bassanezi et al, 2003).…”

Section: The Introduction Of T Citricida In the Caribbeanmentioning

confidence: 99%

“…For example, based on spatial pattern results, it is possible to take the decision to use a different sampling procedure for CSD detection than the one used for CT detection. In addition, taking into account the reported differences between tristeza in Cuba and in other countries of the Caribbean Basin (Batista et al, 2008), the comparative epidemiology of CSD and tristeza epidemics should reinforce and/or clarify concepts about the spread of these diseases. The objectives of this study were to test the hypothesis that epidemiological patterns of CSD in Brazil and citrus tristeza in Cuba are indistinguishable and to improve our knowledge regarding the possible causal agents of CSD.…”

Section: The Introduction Of T Citricida In the Caribbeanmentioning

confidence: 99%

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Comparative epidemiology of citrus tristeza in Cuba and citrus sudden death in Brazil

Batista¹,

Bassanezi²,

Laranjeira³

2008

Trop. plant pathol.

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Citrus Sudden Death (CSD) is a disease of unknown etiology, reported only in Brazil. Due to similarities between the epidemiology of CSD in Brazil and Citrus Tristeza (CT) in other countries, this work aimed to compare CSD and CT in Cuba. Besides testing the hypothesis of shared epidemiological patterns, the data generated can be used to carry out control strategies if CSD breaks out in Cuba. Seven epidemic data sets were statistically compared for each disease by analyzing their progress in time and the dependence among symptomatic or infected plants at two levels of spatial scale. CSD and CT epidemics could not be differentiated based on mean progress rate, area under disease progress curves, proportion of aggregated sequences (Ordinary runs test), index of Dispersion (D) or both parameters, log (A) and b, of the binary power law model. The progress rate of both diseases varied from low (0.0003/month) to very high (0.045/month). The proportion of aggregated sequences was low (<0.16) and D, log(A) and b values indicated aggregation in groups of 4 to 16 plants for both diseases. These results suggest that measures currently used for CT eradication and management in Cuba may be applied in case of an eventual introduction of CSD in that country. Keywords: Citrus tristeza virus, CTV, Closterovirus, Citrus sudden death associated virus, CSDaV, temporal pattern, spatial pattern. RESUMO Epidemiologia comparativa da tristeza dos citros em Cuba e morte súbita dos citros no BrasilA morte súbita dos citros (MSC) é uma doença de causa desconhecida relatada apenas no Brasil. Em função de semelhanças com a tristeza em outros países, objetivou-se comparar a MSC com a tristeza em Cuba. Além de testar a hipótese de similaridade dos padrões epidemiológicos, os dados gerados podem ser utilizados no delineamento de estratégias de controle no caso de uma eventual introdução da MSC em Cuba. Sete epidemias de cada doença foram comparadas estatisticamente por meio de análise do progresso no tempo e da dependência entre plantas sintomáticas ou infectadas em dois níveis de escala espacial. As epidemias de MSC e tristeza não puderam ser distinguidas com base na taxa média de progresso, na área abaixo da curva de progresso da doença, na proporção de seqüências agregadas (Ordinary Runs test), no índice de dispersão (D) ou nos parâmetros log(A) e b após ajuste da lei de Taylor binária. A taxa de progresso das epidemias de ambas as doenças foi variável, de baixa (0,0003/mês) a muito alta (0,045/mês). A proporção de seqüências agregadas foi baixa (<0,16) e os valores de D, log(A) e b indicaram agregação em grupos de 4 a 16 plantas tanto para a MSC quanto para a tristeza. Esses resultados indicam que as atuais medidas usadas no programa de erradicação e manejo da tristeza em Cuba poderiam ser usadas na eventualidade da introdução da MSC nesse país. Palavras-chave: Citrus tristeza virus, CTV, Closterovirus, Citrus sudden death associated virus, CSDaV, padrão temporal, padrão espacial.

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Section: Resultsmentioning

confidence: 99%

“…A model with a good R 2 was accepted as appropriate only if its residuals showed no detectable patterns. Results for CT have been described elsewhere (Batista et al, 2008).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: The Introduction Of T Citricida In the Caribbeanmentioning

confidence: 99%

Section: The Introduction Of T Citricida In the Caribbeanmentioning

confidence: 99%

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Comparative epidemiology of citrus tristeza in Cuba and citrus sudden death in Brazil

Batista¹,

Bassanezi²,

Laranjeira³

2008

Trop. plant pathol.

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Temporal and spatial dynamics of ascochyta blight caused by Ascochyta fabae speg. In faba bean fields in Tunisia

Youssef

Chaar

Bessaidi

et al. 2020

Australasian Plant Pathol.

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Citrus leaf blotch virus in Cuba: first report and partial molecular characterization

Hernández-Rodríguez

Pérez-Castro

García-García

et al. 2016

Trop. plant pathol.

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Citrus leaf blotch virus (CLBV) infection has been detected in citrus cultivars from several countries including the USA, Spain, Italy, Japan and New Zealand. To investigate whether CLBV is present in Cuba, a survey was conducted in seven commercial citrus fields of the country during 2007-2013. In all, 1940 trees were inspected and samples of two symptomatic and 18 asymptomatic sources were tested by biological indexing and molecular techniques for virus detection. Bark patches from the two symptomatic Nagami and Round kumquat sources induced chlorotic blotching and vein clearing in Dweet tangor, and only for the Round kumquat inoculums, stem pitting in Etrog citron. RT-PCR assays using specific primers to amplify fragments of polymerase (RdRp), movement (MP) and coat protein (CP) genes of CLBVyielded amplicons with the expected sizes using RNA from both sources as a template. Viral RNA was further detected by a non-radioactive dot-blot hybridization assay using a mixture of specific probes derived from a Spanish isolate of CLBV. Sequences from RdRp, MP, and CP amplicons showed identical nucleotide identities for the two Cuban isolates, and higher than 95.9 % with isolates from Spain, New Zealand and the USA.

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Spatiotemporal dynamics of Citrus tristeza virus in Cuba

Cited by 5 publications

References 15 publications

Comparative epidemiology of citrus tristeza in Cuba and citrus sudden death in Brazil

Comparative epidemiology of citrus tristeza in Cuba and citrus sudden death in Brazil

Temporal and spatial dynamics of ascochyta blight caused by Ascochyta fabae speg. In faba bean fields in Tunisia

Citrus leaf blotch virus in Cuba: first report and partial molecular characterization

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