Spatiotemporal distributions of sulfonamide and tetracycline resistance genes and microbial communities in the coastal areas of the Yangtze River Estuary

Liu, Shuguang; Xu, Qiang; Lou, Sha; Tu, Junbiao; Yin, Wenjun; Li, Xin; Jin, Yuchen; Раднаева, Л. Д.; Nikitina, Elena; Махинов, А. Н.; Araruna, José Tavares; Fedorova, Irina Viktorovna

doi:10.1016/j.ecoenv.2023.115025

Cited by 14 publications

(2 citation statements)

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“…PCoA analysis showed that the different clusters were isolated in most of the samples from the NAC group (Group 2) and the control group (Group 1), which indicated that NAC could modify the gut microbiome composition (Figure 3). The ARDB results showed that in the control group (Group 1), the main antibiotic resistance genes were vanrb [22], tetq [23], bl2b-tem [24], aac3iii, vanug and cml-e7 (Supplemental Figure S1). However, following NAC treatment (Group 2), the abundances of antibiotic resistance genes were significantly changed, and the main genes were macb [25], tsnr [26], norm, bl2be-per, vansb and pbp1b (Supplemental Figure S1).…”

Section: β-Diversity Analysis Of Pig Gut Microbiome Compositionmentioning

confidence: 99%

“…The ARDB results showed that in the control group (Group 1), the main antibiotic resistance genes were vanrb [22], tetq [23], bl2b-tem [24], aac3iii, vanug and cml-e7 (Supplemental Figure S1). However, following NAC treatment (Group 2), the abundances of antibiotic resistance genes were significantly changed, and the main genes were macb [25], tsnr [26], norm, bl2be-per, vansb and pbp1b (Supplemental Figure S1).…”

Section: For Peer Reviewmentioning

confidence: 99%

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Metagenomic Sequencing Analysis of the Effects of Acetylcysteine on the Pig Gut Microbiome

Fu,

Tian,

et al. 2023

Microbiology Research

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The gut microbiota is engaged in multiple interactions affecting host health, and gut dysbiosis can lead to many diseases. However, the effects of acetylcysteine (NAC) on the gut microbiome composition in pigs using metagenomic sequencing have not been reported. In this study, we used metagenome sequencing to study the effects of NAC on the pig gut microbiome. Sequencing results showed that microbial diversity was changed after NAC treatment. Antibiotic Resistance Genes Database (ARDB) analysis demonstrated that the main genes modified were macb, tsnr, norm, bl2be-per, vansb and pbp1b in the NAC group. Our data showed that NAC could affect microbial distribution at the phylum, gene and species levels. At the species level, NAC significantly increased the abundances of Megasphaera, Lactobacillus reuteri and Megasphaeraelsdenii and reduced the abundances of Phascolarctobacterium succinatutens, Prevotellacopri and Selenomonasbovis compared with the control group. In addition, Gene Ontology (GO) analysis revealed that in the NAC group, cellular process, metabolic process and single-organism process were the dominant terms. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis demonstrated that RNA transport, MAPK signaling pathway, cell cycle, glycosylphosphatidylinositol (GPI)-anchor biosynthesis and VEGF signaling pathway were the dominant signaling pathways in the NAC group. In conclusion, our results suggest that NAC may modify the piglet gut microbiome composition and these findings might provide a new strategy for maintaining animal and human health in the future.

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