Spatiotemporal distribution and speciation of silver nanoparticles in the healing wound

Abstract

First observation of AgNPs dynamics in the wounds of real patients through elemental imaging and speciation.

Silver distribution in chronic wounds and the healing dynamics of chronic wounds treated with dressings containing silver and octenidine

“…48 Roman et al detected the majority of silver nanoparticles in chronic wounds up to a depth of 1.5 mm. 20 We observed the association of silver with elastic and collagen fibers and localization around capillaries in the chronic wounds. Fredriksson et al observed a similar silver distribution pattern in ex vivo explanted human skin treated with the same silver dressing (Aquacel) as used in this study.…”

“…Enhanced levels of serum albumin in patients with chronic wounds serve for protection against increased silver concentrations in the blood 52 . The speciation of silver presented in a study by Roman et al suggested that silver binds predominantly to chloride in the upper layers (up 45 µm) and to glutathione in the deeper parts of the wound 20 . Therefore, silver‐protein complexes may well provide a silver removal pathway from wounds.…”

“…The histopathological score was defined via the sum of six parameters (Table S1) according to Sultana et al 43 Parameters 1-4 were evaluated for the hematoxylin-eosin stained samples and parameters 5 and 6 were evaluated for the Masson's trichrome stained samples. The healing status was classified according to the histopathological score as poor (6-11), fair (12)(13)(14)(15), and good (16)(17)(18)(19)(20).…”

“…17 Recently, Roman et al analyzed the silver distribution and its chemical fate in the chronic wounds of four patients. 20 The relatively novel octenidine antiseptic, which comprises a cationic, surface-active substance, is also used in the treatment of infected chronic wounds. 21 Octenidine disrupts bacterial cellular membranes via the destabilization of phospholipids.…”

“…One study analyzed the distribution of silver in a burn injury patient 17 . Recently, Roman et al analyzed the silver distribution and its chemical fate in the chronic wounds of four patients 20 …”