Spatiotemporal Control of Migration of Single Cells on a Photoactivatable Cell Microarray

Nakanishi, Jun; Kikuchi, Yukiko; Inoue, Satoshi; Yamaguchi, Kazuo; Takarada, Tohru; Maeda, Mizuo

doi:10.1021/ja070294p

Cited by 118 publications

(81 citation statements)

References 21 publications

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“…Alternatively, cell-repellent moieties have been chemically linked to the silane coating by photo-cleavable groups so that they are released in response to UV light. Similarly, loss of the cell-repellent coating could promote the local attachment of cells in suspension (Kikuchi et al, 2008b), trigger cell migration (Nakanishi et al, 2007) or promote the invasion of new areas by multicellular groups (Kikuchi et al, 2008a). With this method, substrate exposure to UV through a photomask placed in the optical plane of a microscope allowed the addition of new adhesive regions whose size could be as small as 5 mm (Nakanishi et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Reprogramming cell shape with laser nano-patterning

Vignaud¹,

Galland²,

Tseng³

et al. 2012

Journal of Cell Science

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SummaryCell shape in vitro can be directed by geometrically defined micropatterned adhesion substrates. However conventional methods are limited by the fixed micropattern design, which cannot recapitulate the dynamic changes of the cell microenvironment. Here, we manipulate the shape of living cells in real time by using a tightly focused pulsed laser to introduce additional geometrically defined adhesion sites. The sub-micrometer resolution of the laser patterning allowed us to identify the critical distances between cell adhesion sites required for cell shape extension and contraction. This easy-to-handle method allows the precise control of specific actin-based structures that regulate cell architecture. Actin filament bundles or branched meshworks were induced, displaced or removed in response to specific dynamic modifications of the cell adhesion pattern. Isotropic branched actin meshworks could be forced to assemble new stress fibers locally and polarised in response to specific geometrical cues.

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Section: Introductionmentioning

confidence: 99%

Reprogramming cell shape with laser nano-patterning

Vignaud¹,

Galland²,

Tseng³

et al. 2012

Journal of Cell Science

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“…42,43 Micro-fabricated cell array devices are in high demand owing to their importance in cell-to-cell interaction, drug testing, and neuronal signaling study. [44][45][46][47][48] In this study, HeLa cells in specific micro-domains were cultured to demonstrate the feasibility of a fabricated microculture platform in cell patterning. Due to the exposed glass surface surrounded by the nonadhesive areas, cells grew in geometrical microtraps.…”

Section: Discussionmentioning

confidence: 99%

Dynamic photopatterning of cellsin situby Q-switched neodymium-doped yttrium ortho-vanadate laser

Deka¹,

Okano²,

Kao³

2013

J. Biomed. Opt

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Abstract. Cellular micropattering has been increasingly adopted in quantitative biological experiments. A Q-switched pulsed neodymium-doped yttrium ortho-vanadate (Nd∶YVO 4 ) laser directed in-situ microfabrication technique for cell patterning is presented. A platform is designed uniquely to achieve laser ablation. The platform is comprised of thin gold coating over a glass surface that functions as a thermal transducer and is over-layered by a cell repellant polymer layer. Micropatterns are engraved on the platform, subsequently exposing specific cell adhesive micro-domains by ablating the gold-polymer coating photothermally. Experimental results indicate that the proposed approach is applicable under culture conditions, viable toward cells, and has a higher engraving speed. Possible uses in arraying isolated single cells on the platform are also shown. Additionally, based on those micro-patterns, dynamic cellular morphological changes and migrational speed in response to geometrical barriers are studied to demonstrate the potential applications of the proposed approach. Our results further demonstrate that cells in narrower geometry had elongated shapes and higher migrational speed than those in wider geometry. Importantly, the proposed approach will provide a valuable reference for efforts to study single cell dynamics and cellular migration related processes for areas such as cell division, wound healing, and cancer invasion. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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“…Those parameters have been found to be quite important in cell survival, 15 proliferation, 16 differentiation, 17 cell migration, 18,19 cytokinesis, 20 and cell polarity. 21,22 In addition, keeping single cells separated by cell micropatterning techniques has been used to analyze variations in gene expression kinetics among individual cells.…”

Section: ·1 Cellular Analysesmentioning

confidence: 99%

“…2a). 19,67,68 "Caged" means "protected by a photocleavable protecting group". We have termed this dynamic substrate as a caged culture substrate.…”

Section: ·3 Substrates Responsive To Lightmentioning

confidence: 99%

See 1 more Smart Citation

Recent Advances in Cell Micropatterning Techniques for Bioanalytical and Biomedical Sciences

et al. 2008

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Cell micropatterning is a method for controlling the placement of living cells on a substrate surface. [1][2][3][4][5][6][7][8] It is important for a wide range of applications, such as tissue engineering, cellbased drug screening, and fundamental cell biology studies. Most cell micropatterning methods fall into three categories based on strategy: (1) seeding cells on a chemically patterned surface of different cell adhesiveness, (2) seeding cells on a topographically patterned surface, or (3) directed delivery of cells onto discrete regions of a substrate. Although the latter two categories are also important, this review mainly focuses on the first category. This is because it is the most common strategy and it provides tools to study fundamental aspects of cell adhesion at the single protein/receptor molecule level. 9There are excellent reviews that also cover the latter two categories. 3,8,10 We first introduce some of the important applications of cell micropatterning in general. We then describe methods for micropatterning the cell adhesiveness, referring to materials that promote or inhibit cell adhesion. We then move on to much more sophisticated substrates, so-called "dynamic substrates", whose cell adhesiveness can be changed by an external stimulus, such as heat, voltage and light. As an example of dynamic substrates, we describe a caged culture substrate developed by our group. This type of functional substrate opens up new possibilities in bioanalytical and biomedical sciences. Cell micropatterning is an important technique for a wide range of applications, such as tissue engineering, cell-based drug screening, and fundamental cell biology studies. This paper overviews cell patterning techniques based on chemically modified substrates with different degrees of cell adhesiveness. In particular, the focus is on dynamic substrates that change their cell adhesiveness in response to external stimuli, such as heat, voltage, and light. Such substrates allow researchers to achieve an in situ alteration of patterns of cell adhesiveness, which is useful for coculturing multiple cell types and analyzing dynamic cellular activities. As an example of dynamic substrates, we introduce a dynamic substrate based on a caged compound, where we accomplished a light-driven alteration of cell adhesiveness and the analysis of a single cell's motility.

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Spatiotemporal Control of Migration of Single Cells on a Photoactivatable Cell Microarray

Cited by 118 publications

References 21 publications

Reprogramming cell shape with laser nano-patterning

Reprogramming cell shape with laser nano-patterning

Dynamic photopatterning of cellsin situby Q-switched neodymium-doped yttrium ortho-vanadate laser

Recent Advances in Cell Micropatterning Techniques for Bioanalytical and Biomedical Sciences

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