Spatiotemporal changes in the Content and Metabolism of 9, 12, 13 – Trihydorxy-10(E)-Octadecenoic Acid in Tomato (<i>Solanum Lycopersicum</i> L. CV Balkonsar) fruits

Abstract

In a previous study, we showed that 9,12,13-trihydroxy-10(E)-octadecenoic acid which is probably involved in plant defense responses is synthesized in tomato fruits and subjected to metabolism. Its catabolism or conversion was thus further characterized. The endogenous level of 9,12,13-trihydroxy-10(E)-octadecenoic acid decreased in the mesocarp and endocarp during the maturation of tomato fruits. The level of this trihydroxy fatty acid in the epicarp declined till the mature-green stage and then increased during the colour change. Injection of buffered solutions of unlabeled 9,12,13-trihydroxy-10(E)-octadecenoic acid into two weeks old fruits while they were still attached to the plants led to the alteration of contents of ten metabolites. After the infiltration of buffered solutions of isotopically labeled 9,12,13-trihydroxy-10(E)-octadecenoic acid into green unripe tomato fruits which were still attached to the plant, the highest radioactivity was found in compounds much more polar than 9,12,13-trihydroxy-10(E)-octadecenoic acid and whose signals in HPLC-UV-chromatogram coincided with the citric acid peak. These results indicated that 9,12,13-trihydroxy-10(E)-octadecenoic acid could be implicated in the control of tomato fruit maturation and β-oxidation might be one of its degradation pathways.

Effects of bio‐based coatings on the ripening and quality attributes of tomato (Solanum lycopersicum) fruits

“…A severe deficiency of vitamin A can lead to blindness and premature death . It is well known that tomato fruits are rich in antioxidants, vitamins and minerals, but in addition it has recently been shown that they also contain pinellic acid, 9,12,13‐trihydroxy‐10( E )‐octadecenoic acid . This trihydroxy fatty acid has prostaglandin E‐like, antifungal,anti‐allergic, anti‐inflammatory and oral adjuvant activities.…”

Untargeted Metabolomics Reveal Defensome-Related Metabolic Reprogramming in Sorghum bicolor against Infection by Burkholderia andropogonis

“…The hydroxy fatty acids, 15-hydroxylinoleic acid (avenoleic acid), 9,12,13-trihydroxy-10-octadecenoic acid (pinellic acid) and others annotated in Table 1, are classified as oxylipins, synthesised from linoleic acid in cereal crops and in other plants [35,36]. 9,12,13-Trihydroxy-10-octadecenoic acid together with other trihydroxyoctadecenoates were reported to be produced in response to fungal infection, conferring resistance to a spectrum of fungal pathogens.…”

“…9,12,13-Trihydroxy-10-octadecenoic acid together with other trihydroxyoctadecenoates were reported to be produced in response to fungal infection, conferring resistance to a spectrum of fungal pathogens. Previous reports have also highlighted the growth-inhibitory roles of trihydroxy derivatives of linoleic- and linolenic acids to plant fungal pathogens [36,37]. Generally, oxylipins perform defence roles in plant innate immunity as signalling molecules; inducing defence responses or as compounds exhibiting antimicrobial properties [33,38].…”

Epoxyalcohol Synthase Branch of Lipoxygenase Cascade

“…The physiological properties of plant oxylipins have been studied, with unjustifiably great attention being paid to jasmonates [49][50][51][52][53][54][55][56][57][58][59][60][61][62][63], traumatin, and green leaf volatiles (GLVs) [46,[64][65][66][67][68][69][70][71][72][73][74][75][76][77][78]. Much less attention has been paid to other branches of the lipoxygenase cascade, including the formation of epoxy hydroxy derivatives (epoxyalcohols) and trihydroxy derivatives (trihydroxy acids), despite the fact that these compounds are found in organisms belonging to different taxa [79][80][81][82][83][84][85][86][87][88][89][90][91][92].…”