Spatio-temporal spread of foot rot (Lasiodiplodia theobromae) in Jatropha curcas L. plantations in Yucatan, Mexico

Uc-Várguez, Alberto; López-Puc, Guadalupe; Gongora-Canul, Carlos; Sebastián, G. Martinez; Aguilera-Cauich, Erick A.

doi:10.1007/s10658-017-1338-y

Cited by 6 publications

(4 citation statements)

References 18 publications

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“…Revista Mexicana de FITOPATOLOGÍA Mexican Journal of Phytopathology la extracción de ADN, siguiendo el protocolo de extracción con DNazol (Life Technologies), con algunas modificaciones. La amplificación por PCR de los fragmentos del gen que codifica para la β-Tubulina se realizó con los oligonucleótidos T1 (5´-AACATGCGTGAGATTGTAAGT-3´) y T2 (5´-TAGTGACCCTTGGCCCAGTTG-3´) (O´Donell & Cigelnik, 1997), Histona 3 con los oligonucleótidos H3-1b (5´-CGCGGCGAGACT-GGATGTCCTT-3´), H3-1a (5´-ACTAAGCAGA-CCGCCCGCAGG-3´) (He et al, 2017), factor de elongación-1α con los oligonucleótidos EF-1 (5′-ATGGGTAAGGA(A/G)GACAAGAC-3′), EF-2 (5′-GGA(G/A)GTACCAGT(G/C)ATCATG-TT-3′) (Herkert et al, 2019) y finalmente la región ITS utilizando oligonucleótidos universales ITS4 (5′-TCCTCCGCTTATTGATATGC-3′) e ITS5 (5′-GGAAGTAAAAGTCGTAACAAGG-3′) siguiendo el protocolo y condiciones de amplificación reportados previamente (Betancourt-Resendes et al, 2012;Uc-Varguez et al, 2018). Los fragmentos amplificados fueron purificados y enviados para su secuenciación a MACROGEN en Korea.…”

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“…La suspensión se centrifugó a 6000 rpm/10 min a 25 °C. El precipitado de conidios se lavó dos veces (5′-GGAAGTAAAAGTCGTAACAAGG-3′), following the protocol and amplification conditions reported earlier (Betancourt-Resendes et al, 2012;Uc-Varguez et al, 2018). The amplified fragments were purified and sent for sequencing to MACROGEN in Korea.…”

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confidence: 99%

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Biological control of Fusarium oxysporum causal agent of gladiolus corm rot by streptomycetes

Rios-Hernández¹,

Uc-Várguez²,

Evangelista-Martínez³

2021

RMF

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Fusarium oxysporum causa la pudrición del cormo en gladiolo provocando pérdidas de hasta el 100%. Se seleccionaron aislados de Fusarium a partir de cormos infectados, se identificó morfológica y molecularmente y se seleccionó un aislado a partir de prueba de patogenicidad. Se seleccionó entre 22 aislados de estreptomicetos una cepa que presentó una actividad antagonista del 40% contra Fusarium. Se obtuvo el Extracto Bioactivo (EB) mediante Fermentación en Fase Sólida y se determinó la concentración mínima inhibitoria (MIC) y concentración mínima letal (MLC) por el método de microdilución. Se obtuvo una MIC para el EB de 0.19 mg mL-1 y una MLC de 0.38 mg mL-1, que se confirmó con un ensayo de germinación de microconidios a 8 h, mostrando un porcentaje de inhibición del 17 y 98% para ¼ y ½ de la MIC. Se evaluó el efecto del EB a 1 y 2 MIC’s de concentración contra la pudrición en cormos de gladiolo infectados, obteniendo un efecto protector en los cormos al mantener su dureza después de 15 días, en comparación con el fungicida Carbendazim. Los resultados indican a Streptomyces sp., como un potencial agente de control biológico contra F. oxysporum.

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Section: Fully Bilingualmentioning

confidence: 99%

Biological control of Fusarium oxysporum causal agent of gladiolus corm rot by streptomycetes

Rios-Hernández¹,

Uc-Várguez²,

Evangelista-Martínez³

2021

RMF

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“…The consequences were a decrease in the availability of fossil fuels and an increase in the price of oil. With the continuing rise in global energy consumption, the energy crisis is having a deeper impact on global social and economic development [1]. In order to set up sustainable development programs and support research on renewable energies, liquid bioenergy production from vegetable oil is an ecological alternative that ensures the development [2].…”

Section: Introductionmentioning

confidence: 99%

“…curcas genotypes are known to have toxins like phorbol esters, curcins and trypsin inhibitor, which confer to the specie a resistance to many pests and various pathogens [5][6][7]. However, monoculture and the increase in cultivated areas have given rise to a number of phytosanitary problems in these recent decades [1], among which the most important are leaf diseases [5,7]. In Burkina Faso, the major leaf diseases affecting J. curcas plantations are necrosis and leaf blight.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Factors Limiting Production of Citrus among Smallholder Farmers in Benue State, Nigeria

2018

JAC

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This study was initiated in order to understand the biochemical mechanisms involved in the resistance of J. curcas to Lasiodiplodia theobromae. Artificial inoculations were done on young seedlings leaves of 8 J. curcas local accessions from Burkina Faso including 4 resistant and 4 susceptible. Two extractions were made for the evaluation of each biochemical parameter: one on the 1 st day before and the second on the 7 th day after inoculation. The study revealed that the response of J curcas to L. threobromae infection is depending to the resistance or the susceptibility of accessions. It showed an increase in the content of biomolecules synthesis such as phenolic, flavonoids, proteins, photosynthetic pigments, salicylic acid and also an increase of catalase and trypsin inhibition activities. In susceptible accessions, the study revealed a decrease in the photosynthetic pigments and an increase in MDA content comparatively to resistant accessions. The ACP performed on the basis of the evaluated biochemical parameters showed that the axis F1 allows the separation of the resistant and the susceptible accessions, and indicated that the resistance of accessions result in the activation of biomolecules synthesis such as phenolic, flavonoids, proteins, photosynthetic pigments, salicylic acid and stimulation of catalase and trypsin inhibition activities. The F2 axis is associated with sugars content and, chymotrypsin inhibition and SOD activities. In susceptible accessions, an increase in MDA content and SOD activity were observed. This study represents an important step in combating leaf fungal diseases by opting to the green technology and by breeding for genotypes with inducing natural defense compounds.

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Agronomy of Jatropha curcas in Mexico

López-Guillén

Bonilla

Valencia

et al. 2019

Jatropha, Challenges for a New Energy Crop

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Spatio-temporal spread of foot rot (Lasiodiplodia theobromae) in Jatropha curcas L. plantations in Yucatan, Mexico

Cited by 6 publications

References 18 publications

Biological control of Fusarium oxysporum causal agent of gladiolus corm rot by streptomycetes

Biological control of Fusarium oxysporum causal agent of gladiolus corm rot by streptomycetes

Assessment of Factors Limiting Production of Citrus among Smallholder Farmers in Benue State, Nigeria

Agronomy of Jatropha curcas in Mexico

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