Spatio‐temporal regulation and cleavage by matrix metalloproteinase stromelysin‐3 implicate a role for laminin receptor in intestinal remodeling during <i>Xenopus laevis</i> metamorphosis

Amano, Tsuyoshi; Fu, Longsheng; Marshak, Anastasia; Kwak, Olivia; Shi, Yun‐Bo

doi:10.1002/dvdy.20511

Cited by 24 publications

(47 citation statements)

References 56 publications

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“…ST3 may accomplish this in part through direct/indirect remodeling of the ECM. In addition, the 67-kDa receptor for laminin (LR), a major ECM component of the basal lamina, was recently identified as a substrate of ST3 using a yeast twohybrid screen (Amano et al, 2005a) and is cleaved by ST3 in metamorphosing tadpole intestine (Amano et al, 2005b). Thus, another possible mechanism of ST3 function could be that TH induces fibroblasts to express ST3, which in turn cleaves LR and alters epithelial cell-ECM interactions.…”

Section: Matrix Metalloproteinasesmentioning

confidence: 99%

Regulation of adult intestinal epithelial stem cell development by thyroid hormone during Xenopus laevis metamorphosis

Ishizuya‐Oka

Shi

2007

Developmental Dynamics

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During amphibian metamorphosis, most or all of the larval intestinal epithelial cells undergo apoptosis. In contrast, stem cells of yet-unknown origin actively proliferate and, under the influence of the connective tissue, differentiate into the adult epithelium analogous to the mammalian counterpart. Thus, amphibian intestinal remodeling is useful for studying the stem cell niche, the clarification of which is urgently needed for regenerative therapies. This review highlights the molecular aspects of the niche using the Xenopus laevis intestine as a model. Because amphibian metamorphosis is completely controlled by thyroid hormone (TH), the analysis of TH response genes serves as a powerful means for clarifying its molecular mechanisms. Although functional analysis of the genes is still on the way, recent progresses in organ culture and transgenic studies have gradually uncovered important roles of cell-cell and cell-extracellular matrix interactions through stromelysin-3 and sonic hedgehog/bone morphogenetic protein-4 signaling pathway in the epithelial stem cell development. Developmental Dynamics 236:3358 -3368, 2007.

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Section: Matrix Metalloproteinasesmentioning

confidence: 99%

Regulation of adult intestinal epithelial stem cell development by thyroid hormone during Xenopus laevis metamorphosis

Ishizuya‐Oka

Shi

2007

Developmental Dynamics

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“…In particular, we have shown that LR is a conserved substrate of ST3 and is cleaved by ST3 in the remodeling intestine during metamorphosis (47,48). Spatiotemporal expression of LR and its cleavage suggest that LR cleavage by ST3 plays a role in T3-induced larval epithelial cell death in the remodeling intestine during metamorphosis (48 (Fig.…”

Section: Transgenic Expression Of St3 Induces Apoptosis Predominantlymentioning

confidence: 72%

“…Although ST3 may cleave ECM proteins strongly in the in vivo environment, these findings suggest that the cleavage of non-ECM proteins is likely important for its biological roles. Consistently, we have recently identified a cell surface receptor, laminin receptor (LR) as an in vivo substrate of ST3 in the tadpole intestine during metamorphosis (47)(48)(49). Analyses of LR expression and cleavage suggest that LR cleavage by ST3 is likely an important mechanism by which ST3 regulates the interaction between the larval epithelial cells and the ECM to induce cell death during intestinal remodeling (47,48).…”

mentioning

confidence: 64%

“…Western Blot and Immunohistochemistry-Proteins were extracted from tail and intestine at the indicated stages of tadpoles as previously described (48). The membrane was incubated with a 1/10,000 dilution of anti-Xenopus LR antibody (48).…”

Section: Quantitative Reverse Transcription-pcr (Qpcr)-totalmentioning

confidence: 99%

“…The membrane was incubated with a 1/10,000 dilution of anti-Xenopus LR antibody (48). Horseradish peroxidase-conjugated secondary antibody was detected by chemiluminescence (Amersham Biosciences TM ECL Plus Western Blotting Detection System).…”

Section: Quantitative Reverse Transcription-pcr (Qpcr)-totalmentioning

confidence: 99%

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Differential Regulation of Cell Type-specific Apoptosis by Stromelysin-3

Mathew

Fiorentino

et al. 2009

Journal of Biological Chemistry

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Matrix metalloproteinases (MMPs) have been extensively studied because of their functional attributes in development and diseases. However, relatively few in vivo functional studies have been reported on the roles of MMPs in postembryonic organ development. Amphibian metamorphosis is a unique model for studying MMP function during vertebrate development because of its dependence on thyroid hormone (T3) and the ability to easily manipulate this process with exogenous T3. The MMP stromelysin-3 (ST3) is induced by T3, and its expression correlates with cell death during metamorphosis. We have previously shown that ST3 is both necessary and sufficient for larval epithelial cell death in the remodeling intestine. To investigate the roles of ST3 in other organs and especially on different cell types, we have analyzed the effect of transgenic overexpression of ST3 in the tail of premetamorphic tadpoles. We report for the first time that ST3 expression, in the absence of T3, caused significant muscle cell death in the tail of premetamorphic transgenic tadpoles. On the other hand, only relatively low levels of epidermal cell death were induced by precocious ST3 expression in the tail, contrasting what takes place during natural and T3-induced metamorphosis when ST3 expression is high. This cell type-specific apoptotic response to ST3 in the tail suggests distinct mechanisms regulating cell death in different tissues. Furthermore, our analyses of laminin receptor, an in vivo substrate of ST3 in the intestine, suggest that laminin receptor cleavage may be an underlying mechanism for the cell type-specific effects of ST3. The extracellular matrix (ECM),3 the dynamic milieu of the cell microenvironment, plays a critical role in dictating the fate of the cell. The cross-talk between the cell and ECM and the timely catabolism of the ECM are crucial for tissue remodeling during development (1). Matrix metalloproteinases (MMPs), extrinsic proteolytic regulators of the ECM, mediate this process to a large extent. MMPs are a large family of Zn 2ϩ -dependent endopeptidases potentially capable of cleaving the extracellular as well as nonextracellular proteins (2-9). The MMP superfamily includes collagenases, gelatinases, stromelysins, and membrane-type MMPs based on substrate specificity and domain organization (2-4). MMPs have been implicated to influence a wide range of physiological and pathological processes (10 -13). The roles of MMPs appear to be very complex. For example, MMPs have been suggested to play roles in both tumor promotion and suppression (13-19). Unfortunately, relatively few functional studies have been carried out in vivo, especially in relation to the mechanisms involved during vertebrate development.Amphibian metamorphosis presents a fascinating experimental model to study MMP function during postembryonic development. A unique and salient feature of the metamorphic process is the absolute dependence on the signaling of thyroid hormone (20 -23). This makes it possible to prevent metamorphosis by simply inhibit...

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Regulation of Adult Intestinal Stem Cells through Thyroid Hormone‐Induced Tissue Interactions during Amphibian Metamorphosis

Ishizuya‐Oka

2014

Cell and Molecular Biology and Imaging of Stem Cells

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Spatio‐temporal regulation and cleavage by matrix metalloproteinase stromelysin‐3 implicate a role for laminin receptor in intestinal remodeling during Xenopus laevis metamorphosis

Cited by 24 publications

References 56 publications

Regulation of adult intestinal epithelial stem cell development by thyroid hormone during Xenopus laevis metamorphosis

Regulation of adult intestinal epithelial stem cell development by thyroid hormone during Xenopus laevis metamorphosis

Differential Regulation of Cell Type-specific Apoptosis by Stromelysin-3

Regulation of Adult Intestinal Stem Cells through Thyroid Hormone‐Induced Tissue Interactions during Amphibian Metamorphosis

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