Spatio-temporal expression pattern and role of the tight junction protein MarvelD3 in pancreas development and function

Abstract

Tight junction complexes are involved in the establishment and maintenance of cell polarity and the regulation of signalling pathways, controlling biological processes such as cell differentiation and cell proliferation. MarvelD3 is a tight junction protein expressed in adult epithelial and endothelial cells. In Xenopus laevis, MarvelD3 morphants present differentiation defects of several ectodermal derivatives. In vitro experiments further revealed that MarvelD3 couples tight junctions to the MEKK1-JNK pathway to regulate cell behaviour and survival. In this work, we found that MarvelD3 is expressed from early developmental stages in the exocrine and endocrine compartments of the pancreas, as well as in endothelial cells of this organ. We thoroughly characterized MarvelD3 expression pattern in developing pancreas and evaluated its function by genetic ablation. Surprisingly, inactivation of MarvelD3 in mice did not alter development and differentiation of the pancreatic tissue. Moreover, tight junction formation and organization, cell polarization, and activity of the JNK-pathway were not impacted by the deletion of MarvelD3.

High expression of MARVELD3 as a potential prognostic biomarker for oral squamous cell carcinoma

“…Members of the TAMP family are predominantly transmembrane proteins. The functions of transmembrane proteins are ensuring the interaction of tight junctions (TJs) strands between adjacent cells ( Heymans et al, 2021 ). The major TJ proteins are classified according to their physiological role in enabling or preventing paracellular transport.…”

“…The Tight junction Associated Marvel Proteins (TAMP) are transmembrane proteins. The TAMP family consists of occludin, tricellulin (also called MARVELD2 ), and MARVEL domain containing 3 ( MARVELD3 ) ( Heymans et al, 2021 ). Its members are associated with tight junctions between cells and epithelial remodeling.…”

BRAFV600E expression in thyrocytes causes recruitment of immunosuppressive STABILIN-1 macrophages

“…Mice were intraperitoneally injected with a saline solution (CTL) or with doxycycline (1µg/g body weight) every 24h during maximum 4 days (Day 1 à Day 4) [22]. The Stabilin-1 knockout mice were generated at the UCLouvain Transgenesis platform as described [23]. Sequences targeted by the crRNAs (crRNA1: 5'-AGGAAAGAATTCAGAACTGG-3'; crRNA2: 5'-GTGATCGTTACCTATCCTCC-3') were chosen to enclose the transcriptional initiation site and the entire first exon (Figure 3A).…”

“…For LYVE-1, CD11b, CD206, STABILIN-1 labelling, frozen sections were used. Tissues were treated as described [23] for permeabilization, blocking and staining steps. For F4/80, LAMP-1 and TG staining, paraffin sections were used.…”

BRAF^V600E Induction in Thyrocytes Triggers Important Changes in the miRNAs Content and the Populations of Extracellular Vesicles Released in Thyroid Tumor Microenvironment

“…Finally, slides were mounted in Dako aqueous Medium (Agilent Technologies) and scanned with the panoramic P250 digital slide scanner (3DHistech). For immunohistofluorescence, thyroid lobes were embedded in gelatin and fozen sections of 6µm were obtained with the cryostat (Thermoscientific) as described [31]. Anti-CD206 primary antibody was incubated overnight at 4°C.…”