Spatio-temporal expression analysis of the calcium-binding protein calumenin in the rodent brain

Abstract

Calumenin is a Ca2+-binding protein that belongs to the CREC superfamily. It contains six EF-hand domains that exhibit a low affinity for Ca2+ as well as an endoplasmic reticulum retention signal. Calumenin exhibits a broad and relatively high expression in various brain regions during development as demonstrated by in situ hybridization. Signal intensity of calumenin is highest during the early development and then declines over time to reach a relatively low expression in adult animals. Immunohistochemistry indicates that at the P0 stage, calumenin expression is most abundant in migrating neurons in the zones around the lateral ventricle. In the brain of adult animals, it is expressed in various glial and neuronal cell types, including immature neurons in subgranular zone of hippocampal dentate gyrus. At the subcellular level, calumenin is identified in punctuate and diffuse distribution mostly in somatic regions where it co-localizes with endoplasmic reticulum (ER) and partially Golgi apparatus. Upon subcellular fractionation, calumenin is enriched in fractions containing membranes and is only weakly present in soluble fractions. This study points to a possible important role of calumenin in migration and differentiation of neurons, and/or in Ca2+ signaling between glial cells and neurons.

Drafting the CLN3 Protein Interactome in SH-SY5Y Human Neuroblastoma Cells: A Label-free Quantitative Proteomics Approach

“…Another protein involved in signalling is Protein-tyrosine phosphatase-like A domain containing 1 protein (PTPLAD1), an integral membrane protein implicated in small GTPase mediated signal transduction and fatty acid biosynthetic process 76,77 . The third novel CLN3 IP, Calumenin (CALU), is a calcium binding protein that is highly expressed in various brain regions during development and functions in protein folding and sorting in the ER 78 .…”

Comparative proteomic analysis of different developmental stages of swamp buffalo testicular seminiferous tubules

“…It has been reported that the protein expression level of CALU in embryonic and neonatal heart is higher than that in adult mouse heart (Sahoo et al., ). Similarly, the expression pattern of CALU protein is highest during the early stages of development in the brain and then declines with age to reach a relatively low expression in adult animals (Vasiljevic et al., ). (Lee, Kwon, & Kim, ) showed that apoptosis was significantly reduced by calumenin overexpression in endoplasmic reticulum stress‐induced cells.…”

Searching for non‐transposable targets of planarian nuclear PIWI in pluripotent stem cells and differentiated cells

“…In other animals, calumenin is a multifunctional Ca 2+ ‐binding protein involved in ER function, cell migration, and other functions (Zheng, Wang, Teng, & Chen, ). In addition, in the mouse brain, neural progenitors specifically express Calu , which might have roles in the migration and differentiation of neurons (Vasiljevic et al., ). Consistently, during planarian regeneration, Djcalu was specifically expressed in differentiating cells, which would be migrating (Figure c,d).…”