Spatio-temporal distribution analysis of circulating genotypes of dengue virus type 1 in western and southern states of India by a one-step real-time RT-PCR assay

Alagarasu, Kalichamy; Patil, J.A.; Kakade, Mahadeo; More, Ashwini; Bote, Minal; Chowdhury, Deepika T.; Seervi, M.; Rajesh, NV; Ashok, M; Anukumar, Balakrishnan; Abraham, Asha Mary; Parashar, Deepti; Shah, P.S.

doi:10.1016/j.meegid.2019.103989

Cited by 4 publications

(4 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Tamil Nadu, GI dominates only during the 2012 outbreak (Cecilia et al, 2017), but the earlier strains of DENV-1 (Vellore 1962-64, Chennai 2014) belong to GV, indicating the emergence of GI. Dual genotype circulation was reported in Vellore (Cecilia et al, 2017), Tirunelveli, Coimbatore, Dindigul, and Triuppur (Alagarasu et al, 2019) districts of Tamil Nadu; interestingly, here we report the cocirculation of Asian (GI) and American/African (GV) of DENV-1 genotype in Madurai, Tamil Nadu.…”

Section: Denv-1 Distributionsupporting

confidence: 65%

“…In India, circulation of DENV-1 genotype was reported to cluster in “American/African” genotype (GV, previously GIII) ( Kukreti et al., 2009 ; Patil et al., 2011 ; Anoop et al., 2012 ; Dash et al., 2015 ) and in GI “Asian” genotype ( Kukreti et al., 2009 ; Alagarasu et al., 2019 ). In Tamil Nadu, GI dominates only during the 2012 outbreak ( Cecilia et al., 2017 ), but the earlier strains of DENV-1 (Vellore 1962–64, Chennai 2014) belong to GV, indicating the emergence of GI.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Emergence of Dengue 4 as Dominant Serotype During 2017 Outbreak in South India and Associated Cytokine Expression Profile

Sankar

Sundari²,

Anand

2021

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Dengue virus (DENV) infection is prevalent in tropical and subtropical regions of the world, which is fatal if untreated symptomatically. Emergence of new genotype within serotypes led to enhanced severity. The objective of the study is to identify the molecular characteristics of the DENV circulated during 2017 outbreak in Tamil Nadu, India, and to investigate the role of inflammatory cytokines in different “serotypes” and in “dengue severity”. A total of 135 suspected samples were tested for DENV infection using IgM, IgG, and qPCR assay; where 76 samples were positive for DENV and analyzed for 12 inflammatory cytokines using ELISA. Serotyping shows 14 DENV-1, 22 DENV-2, 7 DENV-3, and 33 DENV-4, where DENV-4 was predominant. Among 76, 42 isolates were successfully sequenced for C-prM region and grouped. A lineage shift was observed in DENV-4 genotype. Irrespective of serotypes, IFNγ was significantly elevated in all serotypes than control as well as in primary infection than secondary, indicating its role in immune response. GM-CSF and IP-10 were significantly elevated in secondary infection and could be used as prognostic biomarkers for secondary infection. Our observation shows differential cytokine expression profile varied with each serotype, indicating serotype/genotype-specific viral proteins might play a major role in dengue severity. DENV-4 as dominant serotype was reported in Tamil Nadu for the first time during an outbreak with a mixed Th1/Th17 cytokine expression profile that correlated with disease severity. We conclude it is essential to identify circulating viral genotype and their fitness by mutational analysis to correlate with disease severity and immune status, as this correlation will be helpful in diagnostics and therapeutics applications.

show abstract

Section: Denv-1 Distributionsupporting

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Emergence of Dengue 4 as Dominant Serotype During 2017 Outbreak in South India and Associated Cytokine Expression Profile

Sankar

Sundari²,

Anand

2021

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

“…DENV-1 PCR positive samples were tested to find out the proportion of genotype I and V viruses circulating in different places. The real-time RT-PCR was done using genotype specific primers and primers as reported earlier (Alagarasu et al, 2019).…”

Section: Genotyping Of Denv-1 Positive Samples Using Real-time Rt-pcrmentioning

confidence: 99%

“…Phylogenetic analysis of DENV-1 sequences revealed that four samples from Himachal Pradesh, one each from Gujarat and Tamil Nadu clustered with genotype I. E gene sequences of samples collected from Rajasthan, Punjab, Gujarat, Assam, Puducherry, Karnataka, Telangana and Andhra Pradesh belonged to genotype V (Figure 4). Samples which could not be sequenced were tested by a one step real-time RT-PCR assay for genotyping as described earlier (Alagarasu et al, 2019). Among the 71 samples from Maharashtra, 58 belonged to genotype V while 13 were positive for genotype I.…”

Section: Phylogenetic Characterization Of Denv-1mentioning

confidence: 99%

Serotype and genotype diversity of dengue viruses circulating in India: a multi-centre retrospective study involving the Virus Research Diagnostic Laboratory Network in 2018

Alagarasu

Patil

Kakade

et al. 2021

International Journal of Infectious Diseases

Self Cite

View full text Add to dashboard Cite

This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

Serotype‐specific differences in the laboratory parameters among hospitalized children with dengue and genetic diversity of dengue viruses circulating in Tamil Nadu, India during 2017

Rajesh

Alagarasu

Patil

et al. 2019

Journal of Medical Virology

View full text Add to dashboard Cite

In 2017, Tamil Nadu, a southern state, had the second highest number of dengue cases from India. In the present study, the serotype‐specific differences in the clinical manifestations and laboratory parameters among hospitalized children with dengue were investigated and molecular characterization of the circulating dengue virus (DENV) serotypes during 2017 in Tamil Nadu was performed. Eighty children with dengue‐like symptoms consecutively admitted to a tertiary care hospital and positive for DENV NS1 antigen were investigated for DENV serotype utilizing a real‐time reverse transcriptase based polymerase chain reaction assay. Complete envelope (E) gene sequencing of the DENV strains was performed. Seventy samples were positive for serotyping (25 DENV‐1, 17 DENV‐2, six DENV‐3, and 22 DENV‐4). DENV‐4 infections were associated with elevated levels of liver enzymes; Alanine aminotransferase (P = .021) and aspartate aminotransferase (P = .001). However, none of the serotype was associated with any specific clinical features and severe dengue. Asian and American/African genotypes of DENV‐1 were cocirculating. The circulating genotype was cosmopolitan for DENV‐2 with multiple lineages, genotype III for DENV‐3 and genotype I for DENV‐4. Unique mutations were present in the 2017 DENV‐4 isolates. The present study suggests the association of DENV‐4 with elevated liver enzymes in children hospitalized for dengue. Further, the study reports the genetic diversity of DENV circulating in Tamil Nadu during 2017. The study calls for continuous monitoring of the circulating serotypes and genotypes at regional level in India which might result in a region wise database useful in predicting future outbreaks.

show abstract

Spatio-temporal distribution analysis of circulating genotypes of dengue virus type 1 in western and southern states of India by a one-step real-time RT-PCR assay

Cited by 4 publications

References 23 publications

Emergence of Dengue 4 as Dominant Serotype During 2017 Outbreak in South India and Associated Cytokine Expression Profile

Emergence of Dengue 4 as Dominant Serotype During 2017 Outbreak in South India and Associated Cytokine Expression Profile

Serotype and genotype diversity of dengue viruses circulating in India: a multi-centre retrospective study involving the Virus Research Diagnostic Laboratory Network in 2018

Serotype‐specific differences in the laboratory parameters among hospitalized children with dengue and genetic diversity of dengue viruses circulating in Tamil Nadu, India during 2017

Contact Info

Product

Resources

About