Land-use in uplands is an important factor affecting water quality in its respective catchment, and its in uences at the different spatial scales and con gurations warrant further investigation. Here, we selected 26 catchments in the upper Han River (China) and sampled the surface water at the outlet of each catchment in four seasons during 2019. Multivariate statistics were used to identify the relationships between land use characteristics in uplands and water quality in river system. The results indicated that organic pollution (COD Mn ), pH, dissolved oxygen (DO), ions concentration (EC), nutrient (i.e., NH+ 4-N, NO-3-N and dissolved phosphorus (DP) in rivers displayed signi cant seasonal variations.Stepwise regression revealed that landscape metrics such as Patch Density (PD), Landscape Shape Index (LSI), and Splitting Index (SPLIT) were important factors in uencing water quality in rivers regardless of their spatiality and seasonality. Urban was the most frequently appeared land-use type in the best prediction models, and forest area showed a negative correlation with water quality parameters in most cases. Overall, the in uence of land-use on river water quality was slightly higher at reach scale than at catchment and riparian scales. Also, nutrients (i.e., NH+ 4-N, NO-3-N, and DP) in rivers were primarily impacted by the land use characteristic at catchment and riparian scales. Overall, our results suggested that multi-scale explorations would help to achieve a fully understanding on the impacts of land use on river water quality.