Spatio-specific regulation of endocrine-responsive gene transcription by periovulatory endocrine profiles in the bovine reproductive tract

Abstract

In cattle, pro-oestrous oestradiol and dioestrous progesterone concentrations modulate endometrial gene expression and fertility. The aim was to compare the effects of different periovulatory endocrine profiles on the expression of progesterone receptor (PGR), oestrogen receptor 2 (ESR2), oxytocin receptor (OXTR), member C4 of aldo-keto reductase family 1 (AKR1C4), lipoprotein lipase (LPL), solute carrier family 2, member 1 (SLC2A1) and serpin peptidase inhibitor, clade A member 14 (SERPINA14): (1) between uterine horns ipsi- and contralateral to the corpus luteum (CL), (2) between regions of the ipsilateral horn and (3) in the vagina. Endometrium and vagina tissue samples were collected from cows that ovulated a larger (large follicle-large CL, LF-LCL; n=6) or smaller follicle (small follicle-small CL, SF-SCL; n=6) 7 days after oestrus. Cows in the LF-LCL group had a greater abundance of transcripts encoding ESR2, AKR1C4, LPL, SLC2A1 and SERPINA14, but a reduced expression of PGR and OXTR in the endometrium versus the SF-SCL group (PPGR and OXTR was greater in the contralateral compared with the ipsilateral horn (PPGR, ESR2, LPL, SLC2A1 and SERPINA14 (P<0.05). Different periovulatory endocrine profiles, i.e. LF-LCL or SF-SCL, did not influence gene expression in the vagina and had no interaction with inter- or intra-uterine horn gene expression. In conclusion, inter- and intra-uterine horn variations in gene expression indicate that the expression of specific genes in the bovine reproductive tract is location dependent. However, spatial distribution of transcripts was not influenced by distinct periovulatory sex-steroid environments.

<b>Perfis endócrinos peri-ovulatórios influenciam o transporte, metabolismo e disponibilidade de aminoácidos no lúmen uterino de vacas de corte durante o diestro inicial</b>

“…The hormonal strategy employed successfully produced groups of cows presenting distinctly different peri-ovulatory ovarian and endocrine characteristics, as expected and described earlier [26,32,[36][37][38]. Specifically for Experiment 1, cows assigned to the LF-LCL group had larger follicle diameters on D-2, D-1 and D0 when compared to animals from the SF-SCL group (P<0.01; table 2).…”

“…In this context, a previously described in vivo receptivity model was used here [26,32,36,38] aiming to define the AA signatures of the receptive endometrial tissues and histotroph. Using this same model, we were able to manipulate two fundamental aspects of receptivity: compared to the LF-LCL group, fertility was less in the SF-SCL group (i.e., the low receptivity group) [27] and concentration of PGR in the endometrium was greater in that same group on D7 of the estrous cycle [37]. More specifically, data on bovine endometrial tissue transcriptome associated with AA transport has been integrated with information on uterine flushing AA profiles from high receptive (LF-LCL) versus low receptive (SF-SCL) cows groups.…”

<b>Estudo do mecanismo molecular da progesterona e do estradiol no desenvolvimento do endométrio de novilhas Nelore pré-púberes</b>

“…Além da P4, o 17 β estradiol (E2) é um importante componente no desenvolvimento endometrial, principalmente em mecanismos relacionados a proliferação celular RAMOS et al, 2015;ARAÚJO et al, 2015;FRANÇA et al, 2015). O E2 é produzido predominantemente pelas células da granulosa dos folículos ovarianos, tendo suas concentrações aumentadas, principalmente durante as fases de seleção e dominância folicular (DIELEMAN;BEVERS, 1987;XU et al, 1995;KINDER et al, 1996;KULICK et al, 1999;GUINTHER et al, 2000;KOMAR et al, 2001 A expressão e modulação dos diferentes receptores de progesterona e estrógenos acoplados ou não a proteína G, são reportadas nas mais diversas células que compõe o útero humano, e de outras espécies (PERROT-APPLANAT et al, 1985;CLARKE et al, 1987;PRESS;PRESS et al, 1988;GARCIA et al, 1998;LESSEY et al, 1998;PRESS), existindo alguns relatos de expressão no endométrio e oviduto de vacas Nelore cíclicas (LUCIANO et al, 2011;ARAÚJO et al, 2015;GONELLA-DIAZA et al, 2016). Porém, a expressão destes no tecido endometrial de novilhas Nelore prépúberes ainda é desconhecida pela literatura científica.…”

“…A P4 e o E2 regulam o metabolismo celular, e as subsequentes respostas biológicas no desenvolvimento, crescimento e diferenciação das células do endométrio em vacas Nelore (LUCIANO et al, 2011;MESQUITA et al, 2014;RAMOS et al, 2014;MESQUITA et al, 2015;RAMOS et al, 2015;ARAÚJO et al, 2015;FRANÇA et al, 2015).…”

“…As estratégias hormonais adotadas no presente estudo não foram capazes de modular significativamente a abundância de transcritos referentes aos genes: ESR2 ( Entretanto em estudos anteriores, os esteroides ovarianos foram capazes de alterar a modulação desses genes no endométrio de outras espécies, inclusive em vacas cíclicas (ULBRICH et al, 2011;CHENG et al, 2011;SANDEN et al, 2011;YANG et al, 2011;ARAÚJO et al, 2015;.…”