Spatially, Temporally, and Quantitatively Controlled Delivery of Broad Range of Molecules into Selected Cells through Plasmonic Nanotubes

In this research, we demonstrate cell uptake of magneto-electric nanoparticles (MENPs) through nanoelectroporation (NEP) using alternating current (ac)-magnetic field stimulation. Uptake of MENPs was confirmed using focused-ion-beam assisted transmission electron microscopy (FIB-TEM) and validated by a numerical simulation model. The NEP was performed in microglial (MG) brain cells, which are highly sensitive for neuro-viral infection and were selected as target for nano-neuro-therapeutics. When the ac-magnetic field optimized (60 Oe at 1 kHz), MENPs were taken up by MG cells without affecting cell health (viability > 92%). FIB-TEM analysis of porated MG cells confirmed the non-agglomerated distribution of MENPs inside the cell and no loss of their elemental and crystalline characteristics. The presented NEP method can be adopted as a part of future nanotherapeutics and nanoneurosurgery strategies where a high uptake of a nanomedicine is required for effective and timely treatment of brain diseases.

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“…It’s well known that most of the stimuli-responsive electroporation forces may cause cell damage through toxicity37. The MTT-assay (Fig.…”

Section: Resultsmentioning

confidence: 99%

Investigation of ac-magnetic field stimulated nanoelectroporation of magneto-electric nano-drug-carrier inside CNS cells

Kaushik

Nikkhah-Moshaie

Raju

et al. 2017

Sci Rep

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“…9 The process, called plasmonic optoporation, occurs exclusively at the tip of the nanopillars when they are excited by short laser pulses. Such a process, in synergy with other improvements, can be used to achieve major advances in recording technologies and to address the gap in knowledge of acquiring intra- and extra-cellular information about mammalian neurons and cardiomyocytes.…”

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confidence: 99%

Intracellular and Extracellular Recording of Spontaneous Action Potentials in Mammalian Neurons and Cardiac Cells with 3D Plasmonic Nanoelectrodes

et al. 2017

Self Cite

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Three-dimensional vertical micro- and nanostructures can enhance the signal quality of multielectrode arrays and promise to become the prime methodology for the investigation of large networks of electrogenic cells. So far, access to the intracellular environment has been obtained via spontaneous poration, electroporation, or by surface functionalization of the micro/nanostructures; however, these methods still suffer from some limitations due to their intrinsic characteristics that limit their widespread use. Here, we demonstrate the ability to continuously record both extracellular and intracellular-like action potentials at each electrode site in spontaneously active mammalian neurons and HL-1 cardiac-derived cells via the combination of vertical nanoelectrodes with plasmonic optoporation. We demonstrate long-term and stable recordings with a very good signal-to-noise ratio. Additionally, plasmonic optoporation does not perturb the spontaneous electrical activity; it permits continuous recording even during the poration process and can regulate extracellular and intracellular contributions by means of partial cellular poration.

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“…Highlighting a previously described method for generating transient holes in the cell membrane via a plasmonicly created pressure waves 54 , they were able to optically introduce nanocylinder electrodes intracellularly in a non-invasive manner. This allowed them to cleanly transition from extracellular to intracellular recording, with a limited transition time between the two spaces (less than 2 seconds), simultaneously increasing signal intensity.…”

Section: Plasmonics Enabled Optoporation For Intracellular Neural Recmentioning

confidence: 99%

Nongenetic Optical Methods for Measuring and Modulating Neuronal Response

Zimmerman

Tian

2018

ACS Nano

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The ability to sensitively probe and modulate electrical signals at cellular length-scale is a key challenge in the field of electrophysiology. Electrical signals play an integral role in regulating cellular behavior and in controlling biological function. From cardiac arrhythmias to neurodegenerative disorder, maladaptive phenotypes in electrophysiology can result in serious and potentially deadly medical conditions. Understanding how to monitor and control this behavior in a precise and non-invasive fashion represents an important step in developing next-generation therapeutic devices. As we develop a deeper understanding of neural network formation, electrophysiology has the potential to offer fundamental insights into the inner working of the brain. In this perspective, we will first explore traditional methods for examining neural function, briefly touching on recent genetic advances in electrophysiology, before turning to latest innovations in optical sensing and stimulation of action potentials in neurons. We will primarily focus our exploration on nongenetic optical methods, as these provide a high spatiotemporal resolution and can be achieved in a minimally invasive fashion.

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Spatially, Temporally, and Quantitatively Controlled Delivery of Broad Range of Molecules into Selected Cells through Plasmonic Nanotubes

Cited by 90 publications

References 34 publications

Investigation of ac-magnetic field stimulated nanoelectroporation of magneto-electric nano-drug-carrier inside CNS cells

Investigation of ac-magnetic field stimulated nanoelectroporation of magneto-electric nano-drug-carrier inside CNS cells

Intracellular and Extracellular Recording of Spontaneous Action Potentials in Mammalian Neurons and Cardiac Cells with 3D Plasmonic Nanoelectrodes

Nongenetic Optical Methods for Measuring and Modulating Neuronal Response

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