Spatially Resolved Co-Imaging of Polyhalogenated Xenobiotics and Endogenous Metabolites Reveals Xenobiotic-Induced Metabolic Alterations

Huang, Yixuan; Shang, Hailin; Wang, Chao; Cui, Hongyang; Tang, Song; Chang, Hong; Yang, Hui; Jia, Xudong; Wan, Yi

doi:10.1021/acs.est.3c05817

Cited by 4 publications

(1 citation statement)

References 45 publications

(80 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although these methods could provide a wealth of information on the disturbed metabolic pathways induced by pollutants and toxic substances, key spatiotemporal distribution information is normally missing in homogenate sample preparation procedures. Mass spectrometry imaging (MSI) has established itself as a preferential tool for visualizing the spatial distribution of endogenous and exogenous compounds in tissues and single cells, and other merits of label-free and nonspecific detection also prompt additional applications in environmental toxicology. − The migration of docosahexaenoic acid (DHA) within whole tissue from zebrafish can be traced using matrix-assisted laser desorption/ionization MSI (MALDI-MSI), which indicated that DHA migrated into peripheral tissues from intestinal cell membranes . The unique lipid signatures for different organs affected by Niemann–Pick disease type C1 in adult zebrafish can be visualized by MALDI-MSI .…”

Section: Introductionmentioning

confidence: 99%

Mass Spectrometry Imaging Reveals the Morphology-Dependent Toxicological Effects of Nanosilvers on Multiple Organs of Adult Zebrafish (Danio rerio)

Xie,

Li,

Chen

et al. 2024

Environ. Sci. Technol.

View full text Add to dashboard Cite

Nanosilvers with multifarious morphologies have been extensively used in many fields, but their morphology-dependent toxicity toward nontarget aquatic organisms remains largely unclear. Herein, we used matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) to investigate the toxicological effects of silver nanomaterials with various morphologies on spatially resolved lipid profiles within multiple organs in adult zebrafish, especially for the gill, liver, and intestine. Integrated with histopathology, enzyme activity, accumulated Ag contents and amounts, as well as MSI results, we found that nanosilvers exhibit morphology-dependent nanotoxicity by disrupting lipid levels and producing oxidative stress. Silver nanospheres (AgNSs) had the highest toxicity toward adult zebrafish, whereas silver nanoflakes (AgNFs) exhibited greater toxicity than silver nanowires (AgNWs). Levels of differential phospholipids, such as PC, PE, PI, and PS, were associated with nanosilver morphology. Notably, we found that AgNSs induced greater toxicity in multiple organs, such as the brain, gill, and liver, while AgNWs and AgNFs caused greater toxicity in the intestine than AgNSs. Lipid functional disturbance and oxidative stress further caused inflammation and membrane damage after exposure to nanosilvers, especially with respect to sphere morphology. Taken together, these findings will contribute to clarifying the toxicological effects and mechanisms of different morphologies of nanosilvers in adult zebrafish.

show abstract