Spatially resolved chemical analysis with an NSOM-based laser desorption microprobe

Kossakovski, Dmitri; O'Connor, Seamus; Widmer, M.; Baldeschwieler, John D.; Beauchamp, J. L.

doi:10.1016/s0304-3991(97)00054-5

Cited by 38 publications

(30 citation statements)

References 9 publications

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“…133 From our point of view, SNOM is a field where the interaction between modeling and experiment can be extremely fruitful. This interaction, however, has not yet been as successful as one might wish.…”

Section: Perspectivesmentioning

confidence: 99%

Scanning near-field optical microscopy with aperture probes: Fundamentals and applications

Hecht

Sick

Wild

et al. 2000

The Journal of Chemical Physics

698

440

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In this review we describe fundamentals of scanning near-field optical microscopy with aperture probes. After the discussion of instrumentation and probe fabrication, aspects of light propagation in metal-coated, tapered optical fibers are considered. This includes transmission properties and field distributions in the vicinity of subwavelength apertures. Furthermore, the near-field optical image formation mechanism is analyzed with special emphasis on potential sources of artifacts. To underline the prospects of the technique, selected applications including amplitude and phase contrast imaging, fluorescence imaging, and Raman spectroscopy, as well as near-field optical desorption, are presented. These examples demonstrate that scanning near-field optical microscopy is no longer an exotic method but has matured into a valuable tool.

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Section: Perspectivesmentioning

confidence: 99%

Scanning near-field optical microscopy with aperture probes: Fundamentals and applications

Hecht

Sick

Wild

et al. 2000

The Journal of Chemical Physics

698

440

View full text Add to dashboard Cite

show abstract

“…In an early report of near-field laser MS, direct LDI was achieved by aperture near-field LDI in vacuum [112]. The scanning head of a near-field microscope was mounted in a vacuum chamber and was used to place an etched uncoated probe approximately 10 nm from the sample surface.…”

Section: Near-field Laser Ablationmentioning

confidence: 99%

High resolution laser mass spectrometry bioimaging

Murray

Seneviratne

Ghorai

2016

Methods

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MSI (MSI) was introduced more than five decades ago with secondary ion mass spectrometry (SIMS) and a decade later with laser desorption/ionization (LDI) mass spectrometry (MS). Large biomolecule imaging by matrix-assisted laser desorption/ionization (MALDI) was developed in the 1990s and ambient laser MS a decade ago. Although SIMS has been capable of imaging with a moderate mass range at sub-micrometer lateral resolution from its inception, laser MS requires additional effort to achieve a lateral resolution of 10 μm or below which is required to image at the size scale of single mammalian cells. This review covers untargeted large biomolecule MSI using lasers for desorption/ionization or laser desorption and post-ionization. These methods include laser microprobe (LDI) MSI, MALDI MSI, laser ambient and atmospheric pressure MSI, and near-field laser ablation MS. Novel approaches to improving lateral resolution are discussed, including oversampling, beam shaping, transmission geometry, reflective and through-hole objectives, microscope mode, and near-field optics.

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“…SNOM-MS can be performed at atmospheric pressure and ablated material (ions and neutrals) is sampled into the MS vacuum chamber with a closely-positioned orifice, after which additional ions may be generated from the abundant neutrals by electron impact ionization. A TOF-MS system equipped with this desorption/ionization apparatus allowed researchers to analyze atoms and molecules including acetylcholine or 2,5-dihydroxybenzoic acid [159]. Zhu, Zenobi and colleagues [17] have recently shown that SNOM-MS transfers approximately 10% of all ablated material into the mass spectrometer compared to the ~1% ion sampling efficiency of AP-MALDI, a significant improvement.…”

Section: Other Mass Spectrometric Techniques Applied To Single Celmentioning

confidence: 99%

Mass spectrometry imaging and profiling of single cells

Lanni

Rubakhin

Sweedler

2012

Journal of Proteomics

177

145

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Mass spectrometry imaging and profiling of individual cells and subcellular structures provide unique analytical capabilities for biological and biomedical research, including determination of the biochemical heterogeneity of cellular populations and intracellular localization of pharmaceuticals. Two mass spectrometry technologies—secondary ion mass spectrometry (SIMS) and matrix assisted laser desorption ionization mass spectrometry (MALDI MS)—are most often used in micro-bioanalytical investigations. Recent advances in ion probe technologies have increased the dynamic range and sensitivity of analyte detection by SIMS, allowing two- and three-dimensional localization of analytes in a variety of cells. SIMS operating in the mass spectrometry imaging (MSI) mode can routinely reach spatial resolutions at the submicron level; therefore, it is frequently used in studies of the chemical composition of subcellular structures. MALDI MS offers a large mass range and high sensitivity of analyte detection. It has been successfully applied in a variety of single-cell and organelle profiling studies. Innovative instrumentation such as scanning microprobe MALDI and mass microscope spectrometers enable new subcellular MSI measurements. Other approaches for MS-based chemical imaging and profiling include those based on near-field laser ablation and inductively-coupled plasma MS analysis, which offer complementary capabilities for subcellular chemical imaging and profiling.

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Spatially resolved chemical analysis with an NSOM-based laser desorption microprobe

Cited by 38 publications

References 9 publications

Scanning near-field optical microscopy with aperture probes: Fundamentals and applications

Scanning near-field optical microscopy with aperture probes: Fundamentals and applications

High resolution laser mass spectrometry bioimaging

Mass spectrometry imaging and profiling of single cells

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