Spatially resolved cell atlas of the teleost telencephalon and deep homology of the vertebrate forebrain

Hegarty, Brianna E.; Gruenhagen, George; Johnson, Zachary V.; Baker, Caitlin M.; Streelman, J. Todd

doi:10.1101/2023.07.20.549873

Cited by 3 publications

(1 citation statement)

References 71 publications

(136 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We addressed these questions in the posterior compartment of telencephalic area Dp (pDp) of adult zebrafish, the homologue of piriform cortex 33,34 , which is assumed to function as an autoassociative memory network 35,36 . Dp/piriform cortex are the main targets of the olfactory bulb (OB) and respond to odors with distributed activity that is modified by repeated odor stimulation and learning 37–41 .…”

Section: Introductionmentioning

confidence: 99%

Computational functions of precisely balanced neuronal assemblies in an olfactory memory network

Meissner-Bernard,

Jenkins,

Rupprecht

et al. 2024

Preprint

View full text Add to dashboard Cite

Structured connectivity in the brain organizes information by constraining neuronal dynamics. Theoretical models predict that memories are represented by balanced assemblies of excitatory and inhibitory neurons, but the existence and functions of such EI assemblies are difficult to explore. We addressed these issues in telencephalic area Dp of adult zebrafish, the homolog of piriform cortex, using computational modeling, population activity measurements, and optogenetic perturbations. Modeling revealed that precise balance of EI assemblies is important to prevent not only excessive firing rates ("runaway activity") but also the stochastic occurrence of high pattern correlations ("runaway correlations"). Consistent with model-derived predictions, runaway correlations emerged in Dp when synaptic balance was perturbed by optogenetic manipulations of fast-spiking feedback interneurons. Moreover, runaway correlations were driven by sparse subsets of strongly active neurons, rather than by a general broadening of tuning curves. These results reveal novel computational functions of EI assemblies in an autoassociative olfactory memory network and support the hypothesis that EI assemblies organize information on continuous representational manifolds rather than discrete attractor landscapes.

show abstract

Section: Introductionmentioning

confidence: 99%

Computational functions of precisely balanced neuronal assemblies in an olfactory memory network

Meissner-Bernard,

Jenkins,

Rupprecht

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Adult sex change leads to extensive forebrain reorganization in clownfish

Parker,

Gruenhagen,

Hegarty

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

Sexual differentiation of the brain occurs in all major vertebrate lineages but is not well understood at a molecular and cellular level. Unlike most vertebrates, sex-changing fishes have the remarkable ability to change reproductive sex during adulthood in response to social stimuli, offering a unique opportunity to understand mechanisms by which the nervous system can initiate and coordinate sexual differentiation. This study explores sexual differentiation of the forebrain using single nucleus RNA-sequencing in the anemonefish Amphiprion ocellaris, producing the first cellular atlas of a sex-changing brain. We uncover extensive sex differences in cell type-specific gene expression, relative proportions of cells, baseline neuronal excitation, and predicted inter-neuronal communication. Additionally, we identify the cholecystokinin, galanin, and estrogen systems as central molecular axes of sexual differentiation. Supported by these findings, we propose a model of neurosexual differentiation in the conserved vertebrate social decision-making network spanning multiple subtypes of neurons and glia, including neuronal subpopulations within the preoptic area that are positioned to regulate gonadal differentiation. This work deepens our understanding of sexual differentiation in the vertebrate brain and defines a rich suite of molecular and cellular pathways that differentiate during adult sex change in anemonefish.

show abstract

Adult sex change leads to extensive forebrain reorganization in clownfish

Parker,

Gruenhagen,

Hegarty

et al. 2024

Biol Sex Differ

View full text Add to dashboard Cite

Background Sexual differentiation of the brain occurs in all major vertebrate lineages but is not well understood at a molecular and cellular level. Unlike most vertebrates, sex-changing fishes have the remarkable ability to change reproductive sex during adulthood in response to social stimuli, offering a unique opportunity to understand mechanisms by which the nervous system can initiate and coordinate sexual differentiation. Methods This study explores sexual differentiation of the forebrain using single nucleus RNA-sequencing in the anemonefish Amphiprion ocellaris, producing the first cellular atlas of a sex-changing brain. Results We uncover extensive sex differences in cell type-specific gene expression, relative proportions of cells, baseline neuronal excitation, and predicted inter-neuronal communication. Additionally, we identify the cholecystokinin, galanin, and estrogen systems as central molecular axes of sexual differentiation. Supported by these findings, we propose a model of sexual differentiation in the conserved vertebrate social decision-making network spanning multiple subtypes of neurons and glia, including neuronal subpopulations within the preoptic area that are positioned to regulate gonadal differentiation. Conclusions This work deepens our understanding of sexual differentiation in the vertebrate brain and defines a rich suite of molecular and cellular pathways that differentiate during adult sex change in anemonefish.

show abstract

Spatially resolved cell atlas of the teleost telencephalon and deep homology of the vertebrate forebrain

Cited by 3 publications

References 71 publications

Computational functions of precisely balanced neuronal assemblies in an olfactory memory network

Computational functions of precisely balanced neuronal assemblies in an olfactory memory network

Adult sex change leads to extensive forebrain reorganization in clownfish

Adult sex change leads to extensive forebrain reorganization in clownfish

Contact Info

Product

Resources

About