Spatially expressed WIP genes control Arabidopsis embryonic root development

THE WIP6 TRANSCRIPTION FACTORTOO MANY LATERALSSPECIFIES VEIN TYPE IN C4AND C3GRASS LEAVES

“…The spatial expression of TML genes in procambial initial cells, the temporal restriction of expression to the first few divisions of those cells and the mis-specification of intermediate veins as laterals in mutant leaves (Figures 2, 3, 5, 6, 14 S2, S3 & S4) are all consistent with a role in cell-type specification and/or divisions of procambial cells in the leaf. The mutant phenotype is also consistent with the role inferred from triple wip1/3/6 mutants in Arabidopsis, namely that of a suppressor 33 . A repressor role is further supported by the observation that constitutive expression of AtWIP6 conditions a small leaf phenotype in Arabidopsis 63 .…”

Expression and Functional Analyses of the WIP Gene Family in Arabidopsis

“…It is highly likely that wip4 also plays a redundant role in this tissue, considering the redundant roles they play in embryo development [ 12 ]. Considering the observation of the opposing effect of wip3 in this work, and of WIP1 , WIP3 , and WIP6 in [ 15 ], it is possible that these three genes could have an opposite effect. These functions should be studied in more detail to understand the roles that these genes play in vasculature development.…”

“…Very recently, the role of the WIP family genes in early root development was reported [ 15 ]. Interestingly, even when this work was performed using very young roots, some of the observations are in accordance with those of the work presented here, such as nonautonomous effects, and aspects of their expression (such as pWIP3 and pWIP6 in siliques).…”

“…Interestingly, even when this work was performed using very young roots, some of the observations are in accordance with those of the work presented here, such as nonautonomous effects, and aspects of their expression (such as pWIP3 and pWIP6 in siliques). Moreover, the authors report that the combination of wip1 wip3 and wip6 mutations recovers the affected root phenotype of the triple nww ( ntt wip4 wip5 ) mutant, indicating both redundant and opposing roles among the family members [ 15 ]. Interestingly, the authors found that, for the case of the developing root, the opposite roles are related to the different expression pattern of the genes, and not to the intrinsic activity of the protein [ 15 ].…”

“…Moreover, the authors report that the combination of wip1 wip3 and wip6 mutations recovers the affected root phenotype of the triple nww ( ntt wip4 wip5 ) mutant, indicating both redundant and opposing roles among the family members [ 15 ]. Interestingly, the authors found that, for the case of the developing root, the opposite roles are related to the different expression pattern of the genes, and not to the intrinsic activity of the protein [ 15 ]. It would be very interesting to explore if this is also the case for the effects observed in the present work.…”