Spatial Variations of Bacterial Communities of an Anaerobic Lagoon-Type Biodigester Fed with Dairy Manure

Abstract

Anaerobic digestion technology is being widely employed for sustainable management of organic wastes generated in animal farms, industries, etc. Nevertheless, biodigester microbiome is still considered a “black box” because it is regulated by different physico-chemical and operational factors. In this study, the bacterial diversity and composition in different sites of a full-scale lagoon type biodigester (23,000 m3) fed with dairy manure, viz., the influent, beginning, middle, final and effluent were analyzed. The biodigester registered a total of 1445 OTUs, which demonstrated the complex microbial ecosystem in it. Of them, only six OTUs were shared among all the different sampling points. The most abundant phyla belonged to Firmicutes, Proteobacteria, Latescibacteria and Thermotogae. The Simpson and Shannon index showed that the highest microbial diversity was observed in the beginning point of the biodigester, meanwhile, the lowest diversity was recorded in the middle. Based on the UniFrac distances, microbial communities with high similarity were recorded in the middle and final of the biodigester. It can be clearly observed that bacterial communities varied at the different points of the biodigester. However, based on metagenome predictions using PICRUSt, it was found that independent of the differences in taxonomy and location, bacterial communities maintained similar metabolic functions.

Microbial Communities and Sulfate-Reducing Microorganisms Abundance and Diversity in Municipal Anaerobic Sewage Sludge Digesters from a Wastewater Treatment Plant (Marrakech, Morocco)

“…The Proteobacteria, the Bacteroidetes, the Firmicutes, the Actinobacteria, the Synergistetes, the Euryarchaeota, and Cloacimonetes were the dominant phyla in WWTP Marrakech. These data are similar to those previously obtained using marker gene-based analysis [39,40,52,53,55,[57][58][59]. At the OTU level (Figure 3), OTU 1 (Firmicutes), OTU 2 (Cloacimonetes), and OTU 3 (Bacteroidetes) were the most abundant of the four digesters.…”

Metagenomics and metatranscriptomics reveal broadly distributed, active, novel methanotrophs in the Gulf of Mexico hypoxic zone and in the marine water column

“…Members of Latescibacterota (previously WS3 and candidate phylum Lastescibacteria) were first discovered in a hydrocarbon-contaminated aquifer and have since been found in marine sediments, hydrothermal vents, soil and other hydrocarbon-contaminated environments (Farag et al 2017 ). Recently, members of this clade were shown to encode for fermentation (García-Lozano et al 2019 ) and anaerobic hydrocarbon degradation (Dombrowski et al 2017 ), still none have been reported to encode the capacity for aerobic methane oxidation. The Latescibacterota in this study all have pmoA , SDR and ALDH, although most of those genes recruited minimal DNA and RNA reads.…”

Applying PICRUSt and 16S rRNA functional characterisation to predicting co-digestion strategies of various animal manures for biogas production

“…Moreover, Lourenco et al 42 used PICRUSt for a comparison of the microbiota of the beef cattle both ruminal and fecal. Several authors 43 – 46 have specifically focused on its application in biogas processes.…”