Abstract:ABSTRACT. The longitudiual relaxation time T 1 of native cartilage is frequently assumed to be constant. To redress this, the spatial variation of T 1 in unenhanced healthy human knee cartilage in different compartments and cartilage layers was investigated. Knees of 25 volunteers were examined on a 1.5 T MRI system. A three-dimensional gradient-echo sequence with a variable flip angle, in combination with parallel imaging, was used for rapid T 1 mapping of the whole knee. Regions of interest (ROIs) were defin… Show more
“…Previously, it has been assumed that T 1 of native cartilage is relatively constant (11, 26). However, more recent studies both in vitro and in vivo support our findings (27, 28). Generally, water content of articular cartilage is considered more related to T 2 relaxation time (29–31).…”
Purpose: To investigate the transport of Gd-DTPA 2À in different layers of femoral knee cartilage in vivo.Materials and Methods: T 1 measurements (1.5 Tesla) were performed in femoral knee cartilage of 23 healthy volunteers. The weight-bearing central cartilage was analyzed before contrast and at eight time points after an intravenous injection of Gd-DTPA 2À : 12-60 min (4 volunteers) and 1-4 h (19 volunteers). Three regions of interest were segmented manually: deep, middle, and superficial.Results: Before contrast injection, a depth-wise variation of T 1 was observed with 50% higher values in the superficial region compared with the deep region. In the deep region, the uptake of Gd-DTPA 2À was not detected until 36 min and the concentration increased until 240 min, whereas in the superficial region, the uptake was seen already at 12 min and the concentration decreased after 180 min (P < 0.01). There was a difference between medial and lateral compartment regarding bulk, but not superficial Gd-DTPA 2À concentration. The bulk gadolinium concentration was negatively related to the cartilage thickness (r ¼ À0.68; P < 0.01).
Conclusion:The depth-wise and thickness dependent variations in Gd-DTPA 2 transport influence the interpretation of bulk dGEMRIC analysis in vivo. In thick cartilage, incomplete penetration of Gd-DTPA 2 will yield a falsely too long T 1 .
“…Previously, it has been assumed that T 1 of native cartilage is relatively constant (11, 26). However, more recent studies both in vitro and in vivo support our findings (27, 28). Generally, water content of articular cartilage is considered more related to T 2 relaxation time (29–31).…”
Purpose: To investigate the transport of Gd-DTPA 2À in different layers of femoral knee cartilage in vivo.Materials and Methods: T 1 measurements (1.5 Tesla) were performed in femoral knee cartilage of 23 healthy volunteers. The weight-bearing central cartilage was analyzed before contrast and at eight time points after an intravenous injection of Gd-DTPA 2À : 12-60 min (4 volunteers) and 1-4 h (19 volunteers). Three regions of interest were segmented manually: deep, middle, and superficial.Results: Before contrast injection, a depth-wise variation of T 1 was observed with 50% higher values in the superficial region compared with the deep region. In the deep region, the uptake of Gd-DTPA 2À was not detected until 36 min and the concentration increased until 240 min, whereas in the superficial region, the uptake was seen already at 12 min and the concentration decreased after 180 min (P < 0.01). There was a difference between medial and lateral compartment regarding bulk, but not superficial Gd-DTPA 2À concentration. The bulk gadolinium concentration was negatively related to the cartilage thickness (r ¼ À0.68; P < 0.01).
Conclusion:The depth-wise and thickness dependent variations in Gd-DTPA 2 transport influence the interpretation of bulk dGEMRIC analysis in vivo. In thick cartilage, incomplete penetration of Gd-DTPA 2 will yield a falsely too long T 1 .
“…The exact nature of this relationship is unclear, but is believed to relate more to the PG content of the tissue than the collagen architecture [16]. A systematic survey of native T 1 in different cartilage compartments of healthy human volunteers was undertaken by Wiener et al [17]. In this study, T 1 values were shown to decrease from the superficial cartilage layers to the deep layer, consistent with the dependence of native T 1 values upon the macromolecular construction of cartilage.…”
With increasing life expectancies and the desire to maintain active lifestyles well into old age, the impact of the debilitating disease osteoarthritis (OA) and its burden on healthcare services is mounting. Emerging regenerative therapies could deliver significant advances in the effective treatment of OA but rely upon the ability to identify the initial signs of tissue damage and will also benefit from quantitative assessment of tissue repair in vivo. Continued development in the field of quantitative MRI in recent years has seen the emergence of techniques able to probe the earliest biochemical changes linked with the onset of OA. Quantitative MRI measurements including T 1 , T 2 and T 1r relaxometry, diffusion weighted imaging and magnetisation transfer have been studied and linked to the macromolecular structure of cartilage. Delayed gadolinium-enhanced MRI of cartilage, sodium MRI and glycosaminoglycan chemical exchange saturation transfer techniques are sensitive to depletion of cartilage glycosaminoglycans and may allow detection of the earliest stages of OA. We review these current and emerging techniques for the diagnosis of early OA, evaluate the progress that has been made towards their implementation in the clinic and identify future challenges in the field.
“…T1, T1r, T2, and T2* relaxation times displayed concordant regional and zonal changes upon loading. In the unloaded state, T1 values were found to be quite variable, which has been demonstrated before (30). T1 relaxation time is considered more related to the PG than to the collagen content, although the exact biochemical correlate remains unclear (31).…”
Purpose To determine if multiparametric magnetic resonance (MR) imaging mapping can be used to quantify the response to loading of histologically intact human knee cartilage. Materials and Methods Institutional review board approval and written informed consent were obtained. Twenty macroscopically intact cartilage-bone samples were obtained from the central lateral femoral condyles in 11 patients undergoing total knee replacement. A clinical 3.0-T MR imaging system was used to generate T1, T1ρ, T2, and T2* maps with inversion recovery, spin-lock multiple gradient-echo, multiple spin-echo, and multiple gradient-echo sequences. Serial mapping was performed at three defined strain levels (strain 0 [δ], 0%; strain 1 [δ], 19.8% ± 4.6 [standard deviation]; strain 2 [δ], 39.5% ± 9.3) by using displacement-controlled static indentation loading. The entire sample and specific cartilage zones (superficial zone [SZ], transitional zone [TZ], and deep zone [DZ]) and regions (subpistonal area [SPA] and peripistonal area [PPA]) were defined as regions of interest. Upon log transformation, repeated measures analysis of variance was used to detect groupwise regional and zonal differences. Load-induced relative changes were determined and analyzed by using paired Student t test and Spearman correlation. Biomechanical testing (unconfined compression) and histologic assessment (Mankin score) served as the reference standard. Results All samples were histologically intact. Strain-related decreases were found at the SZ and TZ for T1 and T2*; for T1ρ, increases were seen in all zones; and for T2, increases were seen at the SZ and PPA only. Significant parameter changes in the entire sample depth of SPA versus PPA were found for δ (T1ρ, 14% ± 12 vs 6% ± 9) and δ (T1, -4% ± 5 vs -1% ± 3; T1ρ, 13% ± 12 vs 7% ± 7; T2*, -9% ± 12 vs -2% ± 8). SPA versus PPA changes were significant at the SZ and TZ (T1), TZ and DZ (T1ρ), and SZ (T2*). No significant correlations were found between relative changes and biomechanical or histologic parameters. Conclusion Serial multiparametric MR imaging mapping can be used to evaluate cartilage beyond mere static analysis and may provide the basis for more refined graduation strategies of cartilage degeneration. RSNA, 2016 Online supplemental material is available for this article.
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