Spatial patterns of photosynthesis in thin- and thick-leaved epiphytic orchids: unravelling C3–CAM plasticity in an organ-compartmented way

Rodrigues, Maria Aurineide; Matiz, Alejandra; Cruz, Aline Bertinatto; Matsumura, Aline Tiemi; Takahashi, Cassia Ayumi; Hamachi, Leonardo; Félix, Lucas Macedo; Pereira, Paula Natália; Latansio-Aidar, Sabrina Ribeiro; Aidar, Marcos Pereira Marinho; Demarco, Diego; Freschi, Luciano; Mercier, Helenice; Kerbauy, Gilberto Barbante

doi:10.1093/aob/mct090

Cited by 28 publications

(15 citation statements)

References 59 publications

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“…Water deficit that drives closure of stomata result in reduction in CO 2 uptake and subsequent down-regulation of photosynthetic efficiency [34], which is alleviated in epiphytic orchids by carbon fixation through CAM, while achieving water economy [4] [35] [36]. Epiphytic orchids have also been reported to lose water from the leaf slowly, yet maintain high values of RWC [37].…”

Section: Discussionmentioning

confidence: 99%

Photosynthetic Light Utilization Efficiency, Water Relations and Leaf Growth of C3 and CAM Tropical Orchids under Natural Conditions

Tay¹,

He²,

Yam³

2015

AJPS

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Native orchid species of Singapore in their natural conditions experience stress from high irradiance, high temperatures and periods of extended low rainfall, which impact orchid plant physiology and lead to reduced growth and productivity. In this study, it was found that there was a reduction in photochemical efficiency of photosystem II (PSII) in 6 native orchid species under high light (HL) and Bulbophyllum membranaceum under low light (LL). There was chronic photoinhibition in these 6 orchid species over a period of 3 months after transplanting onto the tree trunks without watering and fertilization, especially in Coelogynes mayeriana and Bulbophyllum membranaceum under both HL and LL. This chronic photoinhibition caused by sustained period of water deficit in their natural conditions was later reversed by natural re-watering conditions from higher rainfall. These results indicate that water deficit has a greater impact on photosynthetic light utilization efficiency than excess light. The present study also showed that after natural rewatering, relative water content (RWC) of leaves and pseudobulbs generally increased. During the natural re-watering, total leaf area also gradually increased and reached maximum expansion after 7 weeks under both HL and LL, with some exceptions due to leaf abscission or decline in total leaf area, possibly a strategy for water conservation.

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Section: Discussionmentioning

confidence: 99%

Photosynthetic Light Utilization Efficiency, Water Relations and Leaf Growth of C3 and CAM Tropical Orchids under Natural Conditions

Tay¹,

He²,

Yam³

2015

AJPS

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“…While adapting to harsh habitats, some orchid species have evolved succulent storage-organs, such as pseudobulbs 8 , 9 , thick leaves 10 , and crassulacean acid metabolism (CAM) 11 , a photosynthetic pathway with high water-use efficiency 12 . Morphological and anatomical studies show that orchid plants possess desirable qualities for mitigating drought stress 10 , 13 , 14 . By measuring physiological indexes and secondary metabolites of Dendrobium moniliforme 15 , Wu et al found that increasing antioxidant enzyme activities and osmolytes play an important role in protecting plants under drought stress.…”

Section: Background and Summarymentioning

confidence: 99%

Transcriptomic profiling for prolonged drought in Dendrobium catenatum

et al. 2018

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Orchid epiphytes, a group containing at least 18,000 species, thrive in habitats that often undergo periodic drought stress. However, few global gene expression profiling datasets have been published for studies addressing the drought-resistant mechanism of this special population. In this study, an experiment involving the effect of continuous drought treatments on an epiphytic orchid, Dendrobium catenatum, was designed to generate 39 mature-leaf-tissue RNA-seq sequencing datasets with over two billion reads. These datasets were validated by a series of quality assessments including RNA sample quality, RNA-seq read quality, and global gene expression profiling. We believe that these comprehensive transcriptomic resources will allow a better understanding of the drought-resistant mechanisms of orchid epiphytes.

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“…In addition to distinctive metabolic and physiological features, CAM species typically possess anatomical traits such as thickened, succulent leaves or stems (Borland et al, 2018). Succulence facilitates the vacuolar storage of malic acid as well as water (Balsamo and Uribe, 1988;Rodrigues et al, 2013), and enhances CO 2 trapping during the daytime decarboxylation of CAM by reducing the intercellular air space of the leaf (Borland et al, 2018). Such anatomical characteristics are likely to impose specialized roles for proteins implicated in the transport of CO 2 and water within the mesophyll and epidermis.…”

Section: Introductionmentioning

confidence: 99%

Peeling back the layers of crassulacean acid metabolism: functional differentiation between Kalanchoë fedtschenkoi epidermis and mesophyll proteomes

et al. 2020

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Summary Crassulacean acid metabolism (CAM) is a specialized mode of photosynthesis that offers the potential to engineer improved water‐use efficiency (WUE) and drought resilience in C3 plants while sustaining productivity in the hotter and drier climates that are predicted for much of the world. CAM species show an inverted pattern of stomatal opening and closing across the diel cycle, which conserves water and provides a means of maintaining growth in hot, water‐limited environments. Recent genome sequencing of the constitutive model CAM species Kalanchoë fedtschenkoi provides a platform for elucidating the ensemble of proteins that link photosynthetic metabolism with stomatal movement, and that protect CAM plants from harsh environmental conditions. We describe a large‐scale proteomics analysis to characterize and compare proteins, as well as diel changes in their abundance in guard cell‐enriched epidermis and mesophyll cells from leaves of K. fedtschenkoi. Proteins implicated in processes that encompass respiration, the transport of water and CO2, stomatal regulation, and CAM biochemistry are highlighted and discussed. Diel rescheduling of guard cell starch turnover in K. fedtschenkoi compared with that observed in Arabidopsis is reported and tissue‐specific localization in the epidermis and mesophyll of isozymes implicated in starch and malate turnover are discussed in line with the contrasting roles for these metabolites within the CAM mesophyll and stomatal complex. These data reveal the proteins and the biological processes enriched in each layer and provide key information for studies aiming to adapt plants to hot and dry environments by modifying leaf physiology for improved plant sustainability.

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Spatial patterns of photosynthesis in thin- and thick-leaved epiphytic orchids: unravelling C3–CAM plasticity in an organ-compartmented way

Cited by 28 publications

References 59 publications

Photosynthetic Light Utilization Efficiency, Water Relations and Leaf Growth of C3 and CAM Tropical Orchids under Natural Conditions

Photosynthetic Light Utilization Efficiency, Water Relations and Leaf Growth of C3 and CAM Tropical Orchids under Natural Conditions

Transcriptomic profiling for prolonged drought in Dendrobium catenatum

Peeling back the layers of crassulacean acid metabolism: functional differentiation between Kalanchoë fedtschenkoi epidermis and mesophyll proteomes

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