Spatial patterns and cell surface clusters in perineuronal nets

Arnst, Nikita; Kuznetsova, Svetlana; Lipachev, Nikita; Shaikhutdinov, Nurislam; Melnikova, A. V.; Мавликеев, М. О.; Uvarov, Pavel; Baltina, Tatyana; Rauvala, Heikki; Osin, Yu. N.; Kiyasov, Andrey P.; Paveliev, Mikhail

doi:10.1016/j.brainres.2016.07.020

Cited by 13 publications

(30 citation statements)

References 34 publications

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“…Free-floating modification of the previously described PNN staining protocol (Arnst et al 2016) was used in the present study. The biotinylated WFA (Vector Lab, USA) was used at a 1:500 dilution and the Alexa Fluor 633-conjugated streptavidin at a 1:100 dilution.…”

Section: Spinal Cord Staining Proceduresmentioning

confidence: 99%

Quantitative changes in perineuronal nets in development and posttraumatic condition

et al. 2019

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Perineuronal net (PNN) is a highly structured portion of the CNS extracellular matrix (ECM) regulating synaptic plasticity and a range of pathologic conditions including posttraumatic regeneration and epilepsy. Here we studied Wisteria floribunda agglutinin-stained histological sections to quantify the PNN size and enrichment of chondroitin sulfates in mouse brain and spinal cord. Somatosensory cortex sections were examined during the period of PNN establishment at postnatal days 14, 21 and 28. The single cell PNN size and the chondroitin sulfate intensity were quantified for all cortex layers and specifically for the cortical layer IV which has the highest density of PNN-positive neurons. We demonstrate that the chondroitin sulfate proteoglycan staining intensity is increased between P14 and P28 while the PNN size remains unchanged. We then addressed posttraumatic changes of the PNN expression in laminae 6 and 7 of cervical spinal cord following hemisection injury. We demonstrate increase of the chondroitin sulfate content at 1.6-1.8 mm rostrally from the injury site and increase of the density of PNN-bearing cells at 0.4-1.2 mm caudally from the injury site. We further demonstrate decrease of the single cell PNN area at 0.2 mm caudally from the injury site suggesting that the PNN ECM takes part in the posttraumatic tissue rearrangement in the spinal cord. Our results demonstrate new insights on the PNN structure dynamics in the developing and posttraumatic CNS.

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Section: Spinal Cord Staining Proceduresmentioning

confidence: 99%

Quantitative changes in perineuronal nets in development and posttraumatic condition

et al. 2019

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“…Recent research has focused on PV and PNN and the underlying mechanisms of critical periods and adult plasticity (Fig. ). Thus far, accumulated evidence suggests that resetting the PV network to an immature state and removing PNN from the network can induce critical‐period‐like plasticity or iPlasticity in distinct brain regions .…”

Section: Mechanistic Insights Into Iplasticity: Neuronal and Synapticmentioning

confidence: 99%

“…CSPG has a unique geometric structure of polygonal mesh shapes with the number of vertices varying from three to nine. Sizes of the mesh vary . A reduction in PV (green) and PNN (blue) expression is associated with a plastic and immature network observed in iPlasticity by chronic SSRI treatment.…”

Section: Mechanistic Insights Into Iplasticity: Neuronal and Synapticmentioning

confidence: 99%

“…Cellular regulators of this change in PV expression likely involve molecules maintaining their mature state through PNN, which interact and release factors such as neurotrophins (BDNF and GDNF) and homeoproteins (Otx2) . Recently, the microstructure of PNN has been shown to be of a heterogeneous nature with uniform (nonpolar) and node‐enriched (polar) patterns of chondroitin sulfate distribution within a single mesh . This pattern might be related to the molecular composition of the PNN of the neuronal cell surface and might be functionally connected to the regulation of synaptic signaling processes, including synaptic plasticity .…”

Section: Mechanistic Insights Into Iplasticity: Neuronal and Synapticmentioning

confidence: 99%

“…Recently, the microstructure of PNN has been shown to be of a heterogeneous nature with uniform (nonpolar) and node‐enriched (polar) patterns of chondroitin sulfate distribution within a single mesh . This pattern might be related to the molecular composition of the PNN of the neuronal cell surface and might be functionally connected to the regulation of synaptic signaling processes, including synaptic plasticity . While the function of PNN is still incompletely understood, evidence points towards a role in neuronal protection from oxidative stress .…”

Section: Mechanistic Insights Into Iplasticity: Neuronal and Synapticmentioning

confidence: 99%

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iPlasticity: Induced juvenile‐like plasticity in the adult brain as a mechanism of antidepressants

Umemori

Winkel

Didio

et al. 2018

Psychiatry Clin Neurosci

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The network hypothesis of depression proposes that mood disorders reflect problems in information processing within particular neural networks. Antidepressants (AD), including selective serotonin reuptake inhibitors (SSRI), function by gradually improving information processing within these networks. AD have been shown to induce a state of juvenile‐like plasticity comparable to that observed during developmental critical periods: Such critical‐period‐like plasticity allows brain networks to better adapt to extrinsic and intrinsic signals. We have coined this drug‐induced state of juvenile‐like plasticity ‘iPlasticity.’ A combination of iPlasticity induced by chronic SSRI treatment together with training, rehabilitation, or psychotherapy improves symptoms of neuropsychiatric disorders and issues underlying the developmentally or genetically malfunctioning networks. We have proposed that iPlasticity might be a critical component of AD action. We have demonstrated that iPlasticity occurs in the visual cortex, fear erasure network, extinction of aggression caused by social isolation, and spatial reversal memory in rodent models. Chronic SSRI treatment is known to promote neurogenesis and to cause dematuration of granule cells in the dentate gyrus and of interneurons, especially parvalbumin interneurons enwrapped by perineuronal nets in the prefrontal cortex, visual cortex, and amygdala. Brain‐derived neurotrophic factor (BDNF), via its receptor tropomyosin kinase receptor B, is involved in the processes of synaptic plasticity, including neurogenesis, neuronal differentiation, weight of synapses, and gene regulation of synaptic formation. BDNF can be activated by both chronic SSRI treatment and neuronal activity. Accordingly, the BDNF/tropomyosin kinase receptor B pathway is critical for iPlasticity, but further analyses will be needed to provide mechanical insight into the processes of iPlasticity.

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Application of Machine Learning Methods for Annotating Boundaries of Meshes of Perineuronal Nets

Egorchev,

Kashipov,

Lipachev

et al. 2024

Communications in Computer and Information Science

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Spatial patterns and cell surface clusters in perineuronal nets

Cited by 13 publications

References 34 publications

Quantitative changes in perineuronal nets in development and posttraumatic condition

Quantitative changes in perineuronal nets in development and posttraumatic condition

iPlasticity: Induced juvenile‐like plasticity in the adult brain as a mechanism of antidepressants

Application of Machine Learning Methods for Annotating Boundaries of Meshes of Perineuronal Nets

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