Spatial organization of three‐dimensional cocultures of adriamycin‐sensitive and ‐resistant human breast cancer MCF‐7 cells

Starzec, Anna; Bron, Dominique; Kraemer, Michel; Kouyoumdjian, Jean‐Claude; Moretti, Jean‐Luc; Beaupain, R; Oudar, Olivier

doi:10.1016/s0248-4900(03)00051-0

Cited by 15 publications

(12 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When cultured in the absence of LI90 cells, Huh7 cells were first scattered on the surface, and then, they clustered and formed hepatoma spheroids which are spherical multicellular aggregates as already described when hepatocytes were cultured in other matrix (Fig. 5 b ) 34, 35…”

Section: Resultssupporting

confidence: 58%

Pretargeted radioimmunotherapy in rapidly progressing, metastatic, medullary thyroid cancer

Kraeber-Bodéré

Salaun²,

Oudoux³

et al. 2010

Cancer

View full text Add to dashboard Cite

Medullary thyroid cancer (MTC) patients with localized residual disease and/or distant metastases may survive for several years or rapidly progress and die of their disease. Thus, highly reliable prognostic factors are needed for an early distinction between high-risk patients who need to be treated and low-risk patients who warrant a watch-andwait approach. Calcitonin doubling time is an independent predictor of survival, with a high predictive value in a population of patients who have not normalized their calcitonin, even after repeated surgery. Several imaging methods should be proposed for patients with abnormal residual calcitonin levels persisting after complete surgery: ultrasonography and computed tomography (CT) for neck exploration, and CT for chest, abdomen, and pelvis. Magnetic resonance imaging (MRI) appears to have an advantage over CT for the detection of liver metastases from endocrine tumors. Moreover, MRI appears to be a sensitive imaging technique for detecting the spread of MTC to bone/bone marrow. 2-Fluoro-2-deoxy-D-glucose positron emission tomography/CT could be used for staging patients with progressive MTC, with possible prognostication by standard uptake value quantification. For systemic treatment of patients with rapidly progressing metastatic MTC, chemotherapy is not considered a valid therapeutic option. It is too early to evaluate the potential effectiveness of multikinase inhibitors, although interesting results of phase 2 studies have shown a transient stabilization in 30% to 50% of patients. Pretargeted radioimmunotherapy has been the only innovative treatment modality convincingly showing some survival benefit when compared with a historical untreated control group. Cancer 2010;116(4 suppl):1118-25. V C 2010 American Cancer Society.KEYWORDS: radioimmunotherapy, medullary thyroid carcinoma, pretargeting, calcitonin, carcinoembryonic antigen.Medullary thyroid carcinoma (MTC) accounts for <8% of thyroid cancers. The primary treatment of hereditary or sporadic MTC is total thyroidectomy with dissection of ipsilateral and central lymph nodes, extended in some cases to contralateral dissection. Many patients are cured by this surgical intervention, especially those with familial hereditary MTC and who were operated on before tumor metastatic spread. However, a non-negligible number of patients show persistent disease after primary surgery, as documented by measurable serum calcitonin. 1 Pentagastrin stimulation makes the test extremely sensitive. Three months after surgery, serum calcitonin level is not detectable in >60% of patients without lymph node involvement, as opposed to <20% of patients with lymph node involvement. 2 When a recurrence is localized in the neck or mediastinum, a new surgery can be performed, but is followed by a decrease of calcitonin serum level to undetectable levels in < 1 = 3 of patients. 3 In patients with MTC, the rate of overall survival 10 years after primary surgery is 69%, but it decreases to 10% when distant metastases are present. 4 Patients with ...

show abstract

Section: Resultssupporting

confidence: 58%

Pretargeted radioimmunotherapy in rapidly progressing, metastatic, medullary thyroid cancer

Kraeber-Bodéré

Salaun²,

Oudoux³

et al. 2010

Cancer

View full text Add to dashboard Cite

show abstract

“…We believe that this capability will have major applications for understanding cell-to-cell communication during tissue development and carcinogenesis using actual tissue samples or 3D cell culture models. Examples include understanding genetic variation from cell to cell that arise in solid tumors (23), interactions between drug-resistant and drug-sensitive cells (24), and cell polarity in tissue development and homeostasis (25). However, we do not believe this 3D method will be easily adapted to complete automation, to analyze samples where thousands or more cells per sample require segmentation.…”

Section: Discussionmentioning

confidence: 99%

Whole cell segmentation in solid tissue sections

et al. 2005

View full text Add to dashboard Cite

BackgroundUnderstanding the cellular and molecular basis of tissue development and function requires analysis of individual cells while in their tissue context.MethodsWe developed software to find the optimum border around each cell (segmentation) from two‐dimensional microscopic images of intact tissue. Samples were labeled with a fluorescent cell surface marker so that cell borders were brighter than elsewhere. The optimum border around each cell was defined as the border with an average intensity per unit length greater that any other possible border around that cell, and was calculated using the gray‐weighted distance transform. Algorithm initiation requiring the user to mark two points per cell, one approximately in the center and the other on the border, ensured virtually 100% correct segmentation. Thereafter segmentation was automatic.ResultsThe method was highly robust, because intermittent labeling of the cell borders, diffuse borders, and spurious signals away from the border do not significantly affect the optimum path. Computer‐generated cells with increasing levels of added noise showed that the approach was accurate provided the cell could be detected visually.ConclusionsWe have developed a highly robust algorithm for segmenting images of surface‐labeled cells, enabling accurate and quantitative analysis of individual cells in tissue. © 2005 International Society for Analytical Cytology

show abstract

“…5b). 34,35 When cocultured, 24 hours after plating, Huh7 cells began to form small clusters, whereas LI90 cells were scattered on the matrix (Fig. 5c, day 1).…”

Section: Coculture Of Myofibroblasts and Hepatoma Cellsmentioning

confidence: 99%

Monocyte chemoattractant protein‐1 (MCP‐1)/CCL2 secreted by hepatic myofibroblasts promotes migration and invasion of human hepatoma cells

Dagouassat

Suffee

Hlawaty

et al. 2009

Intl Journal of Cancer

View full text Add to dashboard Cite

The aim of our study was to investigate whether myofibroblasts and the chemokine monocyte chemoattractant protein-1 (MCP-1)/CCL2 may play a role in hepatocellular carcinoma progression. We observed that hepatic myofibroblast LI90 cells express MCP-1/CCL2 mRNA and secrete this chemokine. Moreover, myofibroblast LI90 cell-conditioned medium (LI90-CM) induces human hepatoma Huh7 cell migration and invasion. These effects are strongly reduced when a MCP-1/CCL2-depleted LI90-CM was used. We showed that MCP-1/CCL2 induces Huh7 cell migration and invasion through its G-protein-coupled receptor CCR2 and, to a lesser extent, through CCR1 only at high MCP-1/CCL2 concentrations. MCP-1/CCL2's chemotactic activities rely on tyrosine phosphorylation of focal adhesion components and depend on matrix metalloproteinase (MMP)-2 and MMP-9. Furthermore, we observed that Huh7 cell migration and invasion induced by the chemokine are strongly inhibited by heparin, by b-D-xyloside treatment of cells and by anti-syndecan-1 and -4 antibodies. Finally, we developed a 3-dimensional coculture model of myofibroblast LI90 and Huh7 cells and demonstrated that MCP-1/CCL2 and its membrane partners, CCR1 and CCR2, may be involved in the formation of mixed hepatoma-myofibroblast spheroids. In conclusion, our data show that human liver myofibroblasts act on hepatoma cells in a paracrine manner to increase their invasiveness and suggest that myofibroblast-derived MCP-1/CCL2 could be involved in the pathogenesis of hepatocellular carcinoma.

show abstract

Spatial organization of three‐dimensional cocultures of adriamycin‐sensitive and ‐resistant human breast cancer MCF‐7 cells

Cited by 15 publications

References 21 publications

Pretargeted radioimmunotherapy in rapidly progressing, metastatic, medullary thyroid cancer

Pretargeted radioimmunotherapy in rapidly progressing, metastatic, medullary thyroid cancer

Whole cell segmentation in solid tissue sections

Monocyte chemoattractant protein‐1 (MCP‐1)/CCL2 secreted by hepatic myofibroblasts promotes migration and invasion of human hepatoma cells

Contact Info

Product

Resources

About