Spatial organization of osteoblast fibronectin matrix on titanium surfaces: Effects of roughness, chemical heterogeneity and surface energy

Pegueroles, Marta; Aparicio, Conrado; Bosio, Mattia; Engel, Elisabeth; Gil, F.J.; Planell, Josep A.; Altankov, George

doi:10.1016/j.actbio.2009.07.030

Cited by 106 publications

(95 citation statements)

References 61 publications

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“…However, the mechanisms involved in this observation are difficult to predict in a biological tissue. Adhesion is mainly guided by thermodynamic properties (i.e., roughness and wettability) of the etched dentin surface, which depends on the concavity/convexity of the attained topographical features (Pegueroles et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Ethanol Wet-bonding Technique Sensitivity Assessed by AFM

et al. 2010

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In ethanol wet bonding, water is replaced by ethanol to maintain dehydrated collagen matrices in an extended state to facilitate resin infiltration. Since short ethanol dehydration protocols may be ineffective, this study tested the null hypothesis that there are no differences in ethanol dehydration protocols for maintaining the surface roughness, fibril diameter, and interfibrillar spaces of acidetched dentin. Polished human dentin surfaces were etched with phosphoric acid and waterrinsed. Tested protocols were: (1) water-rinse (control); (2) 100% ethanol-rinse (1-min); (3) 100% ethanol-rinse (5-min); and (4) progressive ethanol replacement (50-100%). Surface roughness, fibril diameter, and interfibrillar spaces were determined with atomic force microscopy and analyzed by one-way analysis of variance and the Student-Newman-Keuls test (α = 0.05). Dentin roughness and fibril diameter significantly decreased when 100% ethanol (1-5 min) was used for rinsing (p < 0.001). Absolute ethanol produced collapse and shrinkage of collagen fibrils. Ascending ethanol concentrations did not collapse the matrix and shrank the fibrils less than absolute ethanol-rinses.KEY WORDS: atomic force microscopy, acidetched dentin, ethanol-saturated, surface topography, water replacement.

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Section: Discussionmentioning

confidence: 99%

Ethanol Wet-bonding Technique Sensitivity Assessed by AFM

et al. 2010

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“…We performed a BCA assay in order to investigate the effect of surface topography on protein adsorption between topographic cues comparable to what was described elsewhere [39][40][41]. So far just a few studies reported protein adsorption on surfaces exhibiting extreme contact angles [20,42,43].…”

Section: Protein Adsorption On Surfacesmentioning

confidence: 99%

Wettability Influences Cell Behavior on Superhydrophobic Surfaces with Different Topographies

et al. 2012

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Surface wettability and topography are recognized as critical factors influencing cell behavior on biomaterials. So far only few works have reported cell responses on surfaces exhibiting extreme wettability in combination with surface topography. The goal of this work is to study whether cell behavior on superhydrophobic surfaces is influenced by surface topography and polymer type. Biomimetic superhydrophobic rough surfaces of polystyrene and poly(l-lactic acid) with different micro/nanotopographies were obtained from smooth surfaces using a simple phase-separation based method. Total protein was quantified and showed a less adsorption of bovine serum albumin onto rough surfaces as compared to smooth surfaces of the same material. The mouse osteoblastic MC3T3-E1 cell line and primary bovine articular chondrocytes were used to study cell attachment and proliferation. Cells attached and proliferate better in the smooth surfaces. The superhydrophobic surfaces allowed cells to adhere but inhibited their proliferation. This study indicates that surface wettability, rather than polymer type or the topography of the superhydrophobic surfaces, is a critical factor in determining cell behavior.

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“…Scarce experimental data exist on the effect of surface nanotopography on protein adsorption but it seems that nanotopography is able to enhance protein adsorption as compared to the same plane chemistry (Khor et al, 2007). Nevertheless, the effect of surface nanotopography on matrix remodelling has not been investigated in the literature and only some qualitative effect has been recently shown (Costa Martínez et al, 2008;Pegueroles et al, 2009). We have developed the technology to prepare different microtopographies with tailored surface chemistry which lead to different cell function as a consequence of the extracellular matrix organisation at the cell-material interface (González-García et al, 2009;Pelham & Wang, 1997).…”

Section: Tissue Engineering 92mentioning

confidence: 99%

“…Cell spreading and motility are higher in stiff substrates than soft ones, what favours cell-cell interaction compared to the cell-material one and leads to more organised cell aggregates (Wong et al, 2003). Cell proliferation increases on stiff surfaces and, in the case of a rigidity gradient on the substrate, cells migrate to stiffer regions (Lo et al, 2000;Bischofs et al, 2003;Pegueroles et al, 2009). This kind of cell behaviour has been found for different cells types (fibroblasts, muscular VSMC cells, chondrocytes and neurons) independently of the protein coating of the substrate (fibronectin, collagen, etc).…”

Section: Tissue Engineering 92mentioning

confidence: 99%