Spatial Organization and Dynamics of RNA Processing in Caulobacter Crescentus

Bayas, Camille; Wang, Jiarui; Lee, Marissa K.; Schrader, Jared M.; Shapiro, Lucy; Moerner, W. E.

doi:10.1016/j.bpj.2017.11.1397

Cited by 1 publication

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“…For example, inner-membrane bound ribosomes in E. coli that are actively engaged in translation ( 89) might play a role in specific translation of inner membrane proteins. In C. crescentus and another alpha-proteobacterium Sinorhizobium meliloti, ribosomes are detected throughout the cell including the nucleoid region (64,90), which is different from the ribosome/nucleoid segregation observed in E. coli and B. subtilis (82,84). Hereby, it was suggested that, mRNA-bound ribosomal subunits show limited mobility in C. crescentus due to the observed limited dispersion of their mRNA targets from their site of transcription (64,90,91).…”

Section: Localized Mrna Translation and Degradationmentioning

confidence: 83%

“…Interestingly, RNase E shows a patchy localization pattern in C. crescentus and the clustered localization of this enzyme is determined by the location of DNA, independent of its mRNA substrates (64,90). Hereby, the localization of RNase E clusters was found to correlate with two subcellular chromosomal positions that encode the highly expressed rRNA genes, indicating that RNase E mediated rRNA processing occurs at the site of rRNA synthesis (90). Although mediated by apparently different mechanisms compared to E. coli, the association of RNase E with DNA in C. crescentus also indicates that there is spatially organized mRNA decay in this organism.…”

Section: Localized Mrna Translation and Degradationmentioning

confidence: 99%

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RNA Localization in Bacteria

Fei

Sharma

2018

Regulating With RNA in Bacteria and Archaea

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Diverse mechanisms and functions of post-transcriptional regulation by small regulatory RNAs (sRNAs) and RNA binding proteins (RBPs) have been described in bacteria. In contrast, little is known about the spatial organization of RNAs in bacterial cells. In eukaryotes, subcellular localization and transport of RNAs play important roles in diverse physiological processes, such as embryonic patterning, asymmetric cell division, epithelial polarity, and neuronal plasticity. It is now clear that bacterial RNAs also can accumulate at distinct sites in the cell. However, due the small size of bacterial cells, localized RNA and translation are more challenging to study in bacterial cells, and the underlying molecular mechanisms of transcript localization are less understood. Here we review the emerging examples of RNAs localized to specific subcellular locations in bacteria, with indications that subcellular localization of transcripts might be important for regulatory processes. Diverse mechanisms for bacterial RNA localization have been suggested, including close association to their genomic site of transcription, or to the localizations of their protein products in translation-dependent or independent processes. We also provide an overview of the state-of-the-art of technologies to visualize and track bacterial RNAs, ranging from hybridization-based approaches in fixed cells to in vivo imaging approaches using fluorescent protein reporters and/or RNA aptamers in single living bacterial cells. We conclude with a discussion of open questions in the field and ongoing technological developments regarding RNA imaging in eukaryotic systems that might likewise provide novel insights into RNA localization in bacteria.

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