Spatial modulation of biomolecules immobilization by fabrication of hierarchically structured PEG-derived brush micropatterns: An versatile cellular microarray platform

Zhao, Haili; Sha, Jiahao; Wu, Tong; Chen, Tao; Chen, Xin; Ji, Huajian; Wang, Yu; Zhu, Huihao; Li, Jingchi; Ma, Yulu

doi:10.1016/j.apsusc.2020.147056

Cited by 11 publications

(9 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Because of their inherent antibiofouling nature, the fabrication of microarrays on the polymer brush modified substrates allows biomolecular immobilization and recognition with low nonspecific interactions, resulting in a significant improvement of the specificity and reproducibility of microarray [77][78][79][80][81][82][83][84][85][86][87][88][89][90][91][92][93][94][95]. In addition, the 3D structure of polymer brush modified substrates provide a high biomolecule immobilization capacity and accessible scaffolds with sufficient space for biomolecule binding, leading to an increase in the sensitivity of microarrays.…”

Section: Polymer Brush-based Microarraysmentioning

confidence: 99%

The Bioanalytical and Biomedical Applications of Polymer Modified Substrates

Sun

2022

Polymers

View full text Add to dashboard Cite

Polymers with different structures and morphology have been extensively used to construct functionalized surfaces for a wide range of applications because the physicochemical properties of polymers can be finely adjusted by their molecular weights, polydispersity and configurations, as well as the chemical structures and natures of monomers. In particular, the specific functions of polymers can be easily achieved at post-synthesis by the attachment of different kinds of active molecules such as recognition ligand, peptides, aptamers and antibodies. In this review, the recent advances in the bioanalytical and biomedical applications of polymer modified substrates were summarized with subsections on functionalization using branched polymers, polymer brushes and polymer hydrogels. The review focuses on their applications as biosensors with excellent analytical performance and/or as nonfouling surfaces with efficient antibacterial activity. Finally, we discuss the perspectives and future directions of polymer modified substrates in the development of biodevices for the diagnosis, treatment and prevention of diseases.

show abstract

Section: Polymer Brush-based Microarraysmentioning

confidence: 99%

The Bioanalytical and Biomedical Applications of Polymer Modified Substrates

Sun

2022

Polymers

View full text Add to dashboard Cite

show abstract

“…Surface chemistry technology is in continuous development, due to the always growing need for robust and functional immobilization methods. One really promising technology has very recently been introduced which makes use of copper-free click chemistry reactions to immobilize fluorescent peptides on brush-like microstructures of a poly(ethylene) glycol (PEG) variant [ 120 ]. Spatial light modulation combined with photoinduced atom transfer radical polymerization (Photo-ATRP) allow to build hierarchical hyperbranched structures of two different polymers and finally immobilize fluorescent FITC-RGD peptides.…”

Section: Novel Technologies and Applicationsmentioning

confidence: 99%

The Role of Surface Chemistry in the Efficacy of Protein and DNA Microarrays for Label-Free Detection: An Overview

et al. 2021

View full text Add to dashboard Cite

The importance of microarrays in diagnostics and medicine has drastically increased in the last few years. Nevertheless, the efficiency of a microarray-based assay intrinsically depends on the density and functionality of the biorecognition elements immobilized onto each sensor spot. Recently, researchers have put effort into developing new functionalization strategies and technologies which provide efficient immobilization and stability of any sort of molecule. Here, we present an overview of the most widely used methods of surface functionalization of microarray substrates, as well as the most recent advances in the field, and compare their performance in terms of optimal immobilization of the bioreceptor molecules. We focus on label-free microarrays and, in particular, we aim to describe the impact of surface chemistry on two types of microarray-based sensors: microarrays for single particle imaging and for label-free measurements of binding kinetics. Both protein and DNA microarrays are taken into consideration, and the effect of different polymeric coatings on the molecules’ functionalities is critically analyzed.

show abstract

“…表面的润湿行为是调节微生物附着力的重要参数之一, 研究发现细菌往往会优先附着在疏水表面 [19] . [20,21] 、聚乙烯醇 (poly(vinyl alcohol), PVA)类 [22] 、聚丙烯酰胺(acrylamide)类 [23] 和聚丙烯酸酯(acrylates)类 [24] 等, 以及基于甜菜碱的两性离子聚合物水凝胶 [25] .…”

Section: 然而临床试验和体外研究结果显示长期使用灭unclassified