Spatial light interference microscopy (SLIM)

Popescu, Gabriel

doi:10.1109/pho.2011.6110797

Cited by 5 publications

(6 citation statements)

References 14 publications

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“…This effect is also present in transport of intensity experiments that share similar experimental conditions (i.e. sample-camera distance < 900μm) [29,30] and other quantitative phase imaging techniques [31,32]. However, lensless phase imagers are mainly used to image biological samples like cells [4][5][6][7][8][9][10] which sizes are typically less than 20μm for which the phase can be correctly computed.…”

Section: Phase Recoverymentioning

confidence: 99%

Compact lensless phase imager

Rostykus¹,

Soulez²,

Unser³

et al. 2017

Opt. Express

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Lensless quantitative phase imaging is of high interest for obtaining a large field of view (FOV), typically the size of the camera chip, to observe biological cell material with high contrast. It has the potential to be widely spread due to its inherent simplicity. However, the tradeoff is the added complexity due to the illumination. Current illumination systems are several centimeters away from the sample, use mechanics to obtain super resolution (i.e., smaller than the detector pixel size) or different illumination directions, and block the view to the sample. In this paper, we propose and demonstrate a side illumination system which reduces the height by an order of magnitude while providing an unobstructed view of the sample. We achieve this by shaping the illumination using multiplexed analog holograms that produce 9 illumination angles. We demonstrate experimentally imaging of phase samples with a FOV of ~17mm 2 .

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Section: Phase Recoverymentioning

confidence: 99%

Compact lensless phase imager

Rostykus¹,

Soulez²,

Unser³

et al. 2017

Opt. Express

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“…The retrieved method can be found in previous reports. 9 The irradiance distribution at each point r ¼ ðx; yÞ in the image plane is a function of the phase shift, namely E Q -T A R G E T ; t e m p : i n t r a l i n k -; e 0 0 1 ; 6 3 ; 5 9 4…”

Section: Image Processingmentioning

confidence: 99%

“…QPI is an emerging method that provides quantitative information about transparent specimens, with nanoscale sensitivity and in label-free mode. 9 Various implementations of QPI have been used recently to study the topography of gametes, assuming a certain refractive index. [10][11][12][13][14][15][16] Rappaz et al 17 reported an experimental procedure to calculate both the refractive index and the cellular thickness from the phase shift using digital holographic microscopy.…”

Section: Introductionmentioning

confidence: 99%

Topography and refractometry of sperm cells using spatial light interference microscopy

et al. 2018

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Characterization of spermatozoon viability is a common test in treating infertility. Recently, it has been shown that label-free, phase-sensitive imaging can provide a valuable alternative for this type of assay. We employ spatial light interference microscopy (SLIM) to perform high-accuracy single-cell phase imaging and decouple the average thickness and refractive index information for the population. This procedure was enabled by quantitative-phase imaging cells on media of two different refractive indices and using a numerical tool to remove the curvature from the cell tails. This way, we achieved ensemble averaging of topography and refractometry of 100 cells in each of the two groups. The results show that the thickness profile of the cell tail goes down to 150 nm and the refractive index can reach values of 1.6 close to the head.

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“…In previous TPM experimental arrangements (Fang‐Yen et al ., ; Popescu, ; Xiu et al ., ), the angle of the reference beam incident into the image plane was fixed. Taking the numerical aperture (NA) and the pixel size of the camera into consideration, the maximum resolvable phase gradient will be reduced.…”

Section: Introductionmentioning

confidence: 99%

Analogous on‐axis interference topographic phase microscopy (AOITPM)

Xiu

Liu

Zhou

et al. 2018

Journal of Microscopy

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The refractive index (RI) of a sample as an endogenous contrast agent plays an important role in transparent live cell imaging. In tomographic phase microscopy (TPM), 3D quantitative RI maps can be reconstructed based on the measured projections of the RI in multiple directions. The resolution of the RI maps not only depends on the numerical aperture of the employed objective lens, but also is determined by the accuracy of the quantitative phase of the sample measured at multiple scanning illumination angles. This paper reports an analogous on-axis interference TPM, where the interference angle between the sample and reference beams is kept constant for projections in multiple directions to improve the accuracy of the phase maps and the resolution of RI tomograms. The system has been validated with both silica beads and red blood cells. Compared with conventional TPM, the proposed system acquires quantitative RI maps with higher resolution (420 nm @λ = 633 nm) and signal-to-noise ratio that can be beneficial for live cell imaging in biomedical applications.

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Spatial light interference microscopy (SLIM)

Cited by 5 publications

References 14 publications

Compact lensless phase imager

Compact lensless phase imager

Topography and refractometry of sperm cells using spatial light interference microscopy

Analogous on‐axis interference topographic phase microscopy (AOITPM)

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