Spatial imaging and speciation of Cu in rice (Oryza sativa L.) roots using synchrotron-based X-ray microfluorescence and X-ray absorption spectroscopy

Lu, Lingli; Xie, Ruohan; Liu, Ting; Wang, Haixing; Hou, Dandi; Du, Yonghua; He, Zhenli; Yang, Xiaoe; Sun, Hui; Tian, Shengke

doi:10.1016/j.chemosphere.2017.02.082

Cited by 47 publications

(19 citation statements)

References 42 publications

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“…In the HTRP experiment the largest concentrations of P in root cross sections was found in the endodermis (Fig. 5 ) in agreement with previous reports in various plant species [ 39 , 41 , 42 , 71 ]. The endodermis represents a key apoplastic barrier to the radial transport of water and ions to the vascular system of the plant and as such it acts as a checkpoint in nutrient delivery to the shoot [ 27 ].…”

Section: Discussionsupporting

confidence: 92%

“…Although P nutrition has been studied in a wide range of plant species, particularly in crops, studies of the tissue-specific distribution of P in roots are rare and typically a serendipitous result of using multi-element analytical techniques. In general, a progressive increase in cellular P from the rhizodermis towards the endodermis, where the majority of P is located, and smaller P concentrations in cells located within the stele has been reported [ 39 – 42 ].…”

Section: Introductionmentioning

confidence: 99%

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Effect of phosphorus supply on root traits of two Brassica oleracea L. genotypes

et al. 2020

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Background: Phosphorus (P) deficiency limits crop production worldwide. Crops differ in their ability to acquire and utilise the P available. The aim of this study was to determine root traits (root exudates, root system architecture (RSA), tissue-specific allocation of P, and gene expression in roots) that (a) play a role in P-use efficiency and (b) contribute to large shoot zinc (Zn) concentration in Brassica oleracea. Results: Two B. oleracea accessions (var. sabellica C6, a kale, and var. italica F103, a broccoli) were grown in a hydroponic system or in a high-throughput-root phenotyping (HTRP) system where they received Low P (0.025 mM) or High P (0.25 mM) supply for 2 weeks. In hydroponics, root and shoot P and Zn concentrations were measured, root exudates were profiled using both Fourier-Transform-Infrared spectroscopy and gas-chromatography-mass spectrometry and previously published RNAseq data from roots was reexamined. In HTRP experiments, RSA (main and lateral root number and lateral root length) was assessed and the tissue-specific distribution of P was determined using micro-particle-induced-X-ray emission. The C6 accession had greater root and shoot biomass than the F103 accession, but the latter had a larger shoot P concentration than the C6 accession, regardless of the P supply in the hydroponic system. The F103 accession had a larger shoot Zn concentration than the C6 accession in the High P treatment. Although the F103 accession had a larger number of lateral roots, which were also longer than in the C6 accession, the C6 accession released a larger quantity and number of polar compounds than the F103 accession. A larger number of P-responsive genes were found in the Low P treatment in roots of the F103 accession than in roots of the C6 accession. Expression of genes linked with "phosphate starvation" was upregulated, while those linked with iron homeostasis were down-regulated in the Low P treatment. Conclusions: The results illustrate large within-species variability in root acclimatory responses to P supply in the composition of root exudates, RSA and gene expression, but not in P distribution in root cross sections, enabling P sufficiency in the two B. oleracea accessions studied.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Effect of phosphorus supply on root traits of two Brassica oleracea L. genotypes

et al. 2020

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“…; Lu et al . ), the speciation of Cu with other compounds cannot be completely excluded. In addition, the binding of Cu to some sulphur‐containing ligands is one mechanism that plants use to detoxify Cu stress (Song et al .…”

Section: Resultsmentioning

confidence: 97%

“…Using this procedure, the spectra were correctly simulated by eight components (Lu et al . ). The corresponding fitting parameters are reported in Table .…”

Section: Resultsmentioning

confidence: 99%

The speciation and distribution characteristics of Cu in Phragmites australis (Cav.) Trin ex. Steudel

Wang

Zhao

et al. 2019

Plant Biol J

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Heavy metal allocation and the mechanism(s) of metal sequestration in different clonal organs, micro-domains and subcellular structures has not been systematically studied for rhizomatous perennial plants. It is thus pertinent to investigate knowledge of the speciation and distribution characteristics of Cu in Phragmites australis to elucidating the mobility of metals in wetland plants after their uptake via root systems so as to facilitate development of strategies to enhance Cu tolerance.• This study investigated the distributions of Cu in P. australis root, stem and leaf using ICP-MS, synchrotron-based X-ray micro-fluorescence and X-ray absorption spectroscopy, then evaluated the effects of Cu on cellular structure and ultrastructure via transmission electron microscopy.• The results indicate a clear preferential localisation of Cu in the roots as compared with the shoots (stems and leaves). The intensity of Cu in the vascular bundles was higher than that in the surrounding epidermis and the endodermis and parenchyma outside the medullary cavity. The dominant chemical form of Cu in P. australis was similar to Cu citrate.• The results suggest that although Cu can be easily transported into the vascular tissues in roots and stems via Cu citrate, most of the metal absorbed by plants is retained in the roots because if its high binding to the cell wall, thus preventing metal translocation to aerial parts of the plants. Therefore, P. australis showed a high capacity to accumulate Cu in roots, being therefore a suitable species for phytostabilisation interventions.

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“…Previous reports on Elsholtzia splendens and Bambusoideae plants showed that the majority of absorbed Cu is accumulated nearby the root rhizodermis as a layer [35,36]. Meanwhile, other studies in Oryza sativa and Commelina communis evidenced a major Cu accumulation in the vascular tissues at the root level [37,38].…”

Section: Discussionmentioning

confidence: 96%

Main Molecular Pathways Associated with Copper Tolerance Response in Imperata cylindrica by de novo Transcriptome Assembly

Vidal

Larama

Riveros

et al. 2021

Plants

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The metallophyte Imperata cylindrica inhabits copper (Cu) polluted soils in large areas from Central Chile. Here, we subjected clonal vegetative plantlets to 300 mg Cu kg−1 of substrate for 21 days to identify the main molecular pathways involved in the response to Cu stress. Transcriptomic analyses were performed for shoots and roots, with and without Cu supply. RNA-Seq and de novo transcriptome assembly were performed to identify the gene response associated with molecular mechanisms of Cu tolerance in I. cylindrica. De novo transcriptome revealed a total of 200,521 transcripts (1777 bp) comprising ~91% complete ultra-conserved genes in the eukaryote and Plantae database. The differentially expressed genes (DEGs) in roots were 7386, with 3558 of them being up-regulated and the other 3828 down-regulated. The transcriptome response in shoots was significantly less, showing only 13 up-regulated and 23 down-regulated genes. Interestingly, DEGs mainly related with actin and cytoskeleton formation, and to a minor degree, some DEGs associated with metal transporters and superoxide dismutase activity in root tissues were found. These transcriptomic results suggest that cytoskeleton could be acting as a mechanism of Cu-binding in the root, resulting in a high Cu tolerance response in this metallophyte, which deserve to be analyzed ultra-structurally. Our study contributes to reinforcing the potential of I. cylindrica as a candidate plant species to be used as a phytoremediation agent in Cu-contaminated environments.

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Spatial imaging and speciation of Cu in rice (Oryza sativa L.) roots using synchrotron-based X-ray microfluorescence and X-ray absorption spectroscopy

Cited by 47 publications

References 42 publications

Effect of phosphorus supply on root traits of two Brassica oleracea L. genotypes

Effect of phosphorus supply on root traits of two Brassica oleracea L. genotypes

The speciation and distribution characteristics of Cu in Phragmites australis (Cav.) Trin ex. Steudel

Main Molecular Pathways Associated with Copper Tolerance Response in Imperata cylindrica by de novo Transcriptome Assembly

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