2022
DOI: 10.1017/s1431927622000228
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Spatial Image Resolution Assessment by Fourier Analysis (SIRAF)

Abstract: Determining spatial resolution from images is crucial when optimizing focus, determining smallest resolvable object, and assessing size measurement uncertainties. However, no standard algorithm exists to measure resolution from electron microscopy (EM) images, though several have been proposed, where most require user decisions. We present the Spatial Image Resolution Assessment by Fourier analysis (SIRAF) algorithm that uses fast Fourier transform analysis to estimate resolution directly from a single image w… Show more

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Cited by 4 publications
(1 citation statement)
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“…The largest frequencies can either be used to estimate the FWHM of the point-spread function (see refs. 47 , 48 and Methods) or assess the quality of the resulting images 49 , 50 . To estimate whether CARE improves the resolution of the noisy images, we first applied an FFT, calculated the radial average of the resulting power spectrum and fitted to a rationalized equation based on a Gaussian point-spread function to extract d m i n , the minimally resolvable distance 49 .…”
Section: Resultsmentioning
confidence: 99%
“…The largest frequencies can either be used to estimate the FWHM of the point-spread function (see refs. 47 , 48 and Methods) or assess the quality of the resulting images 49 , 50 . To estimate whether CARE improves the resolution of the noisy images, we first applied an FFT, calculated the radial average of the resulting power spectrum and fitted to a rationalized equation based on a Gaussian point-spread function to extract d m i n , the minimally resolvable distance 49 .…”
Section: Resultsmentioning
confidence: 99%