Spatial heterogeneity of the metabolic response of RIF‐1 tumors to a vasoactive agent evaluated <i>in vivo</i> by one‐dimensional <sup>31</sup>P chemical‐shift imaging

Bhujwalla, Zaver M.; Blackband, Stephen J.; Wehrle, Janna P.; Glickson, Jerry D.

doi:10.1002/mrm.1910260210

Cited by 4 publications

(2 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vivo 31 P spectra of sc-implanted tumours may show a relatively large PCr signal because of contamination from adjacent muscle tissue, which has high concentration of PCr. Bhujwalla et al [47] have applied one-dimensional chemical-shift imaging to obtain localized 31 P spectra from sc-implanted RIF-1 tumours. The spectra from the slices that were entirely within the tumour did not show a PCr signal, while the spectra from the slices that contained both tumour and normal tissue showed a large PCr signal.…”

Section: Discussionmentioning

confidence: 99%

Heat-induced changes in intracellular Na⁺, pH and bioenergetic status in superfused RIF-1 tumour cells determined by²³Na and³¹P magnetic resonance spectroscopy

Babsky

Hekmatyar

Gorski

et al. 2005

International Journal of Hyperthermia

View full text Add to dashboard Cite

The acute effects of hyperthermia on intracellular Na+ (Nai+), bioenergetic status and intracellular pH (pHi) were investigated in superfused Radiation Induced Fibrosarcoma-1 (RIF-1) tumour cells using shift-reagent-aided 23Na and 31P nuclear magnetic resonance (NMR) spectroscopy. Hyperthermia at 45 degrees C for 30 min produced a 50% increase in Na, a 0.42 unit decrease in pHi and a 40-45% decrease in NTP/P(i). During post-hyperthermia superfusion at 37 degrees C, pHi and NTP/P(i) recovered to the baseline value, but Na initially decreased and then increased to the hyperthermic level 60 min after heating. Hyperthermia at 42 degrees C caused only a 15-20% increase in Nai+. In the presence of 3 microM 5-(N-ethyl-N-isopropyl)amiloride (EIPA), an inhibitor of the Na+/H+ exchanger, the increase in Nai+ during 45 degrees C hyperthermia was attenuated, suggesting that the heat-induced increase in Nai+ was mainly due to an increase in Na+/H+ anti-porter activity. EIPA did not prevent hyperthermia-induced acidification. This suggests that pHi is controlled by other ion exchange mechanisms in addition to the Na+/H+ exchanger. EIPA increased the thermo-sensitivity of the RIF-1 tumour cells only slightly as measured by cell viability and clonogenic assays. The hyperthermia-induced irreversible increase in Nai+ suggests that changes in transmembrane ion gradients play an important role in cell damage induced by hyperthermia.

show abstract

Section: Discussionmentioning

confidence: 99%

Heat-induced changes in intracellular Na⁺, pH and bioenergetic status in superfused RIF-1 tumour cells determined by²³Na and³¹P magnetic resonance spectroscopy

Babsky

Hekmatyar

Gorski

et al. 2005

International Journal of Hyperthermia

View full text Add to dashboard Cite

show abstract

“…The increase in Pi correlated well with the increase in hypoxic fraction (27). When localized 31 P spectroscopy was used, it was noted that there was regional heterogeneity in the response (13).…”

Section: Quantifying Oxygen In the Cell-correlates Of Oxygenationmentioning

confidence: 98%

Measuring Oxygenation In Vivo with MRS/MRI—From Gas Exchange to the Cell

Dunn

2007

Antioxidants & Redox Signaling

View full text Add to dashboard Cite

Oxygen plays a major role as a substrate in metabolic processes in numerous signaling pathways, in redox metabolism, and in free radical metabolism. To study the role of oxygen in normal and pathophysiological states, methods that can be used noninvasively are required. This review examines the potential of nuclear magnetic resonance techniques to study tissue oxygenation. It is written from a systems perspective, looking at detection methods with respect to the path that oxygen takes in the mammalian system-from the lungs, through the vascular system, into the interstitial space, and finally into the cell. Methods discussed range from those that are quantifiable, such as the assessment of spin lattice relaxation time in fluorocarbon solutions, to those that are more correlative, such as assessment of lactate and high energy phosphates. Since the methods vary in their site of application, sensitivity, and specificity to the quantification of oxygen, this review provides examples of how each method has been applied. This may facilitate the reader's understanding of how to optimally apply different methods to study specific biomedical problems.

show abstract

¹H NMR spectroscopy of subcutaneous tumors in mice: Preliminary studies of effects of growth, chemotherapy and blood flow reduction

Shungu¹,

Bhujwalla²,

Wehrle³

et al. 1992

NMR in Biomedicine

View full text Add to dashboard Cite

This study evaluates a number of methods for obtaining 1H NMR spectra with adequate suppression of lipid and water resonances in two subcutaneously implanted transplantable tumor models (RIF-1 and EMT6/SF). Spin-echo spectra with long TEs (270 ms; water suppressed by presaturation) eliminated lipid resonances from 1H spectra of RIF-1 and decreased lipid contamination in spectra of EMT6/SF; however, spectral sensitivity was substantially reduced. A shorter TE (135 ms) increased sensitivity but did not result in adequate suppression of the lipid peaks. In RIF-1, but not EMT6/SF, adequate lipid suppression was achieved by: (i) spatially selective presaturation of lipid, which in this tumor (but not in EMT6/SF) was localized in a thin region along the periphery of the tumor, followed by a 1-D spin-echo chemical shift imaging pulse sequence (TE = 135 ms); and (ii) 2-D spin-echo chemical shift imaging (TE = 270 ms; approximately 2 x 2 x 9 mm3 voxels). Preliminary 1H studies of the RIF-1 tumor indicate that: (i) there are no significant changes in metabolite levels relative to tumor water during 4 days of untreated tumor growth; (ii) tumor response to chemotherapy with 5-fluorouracil results in a decrease in intensity of all metabolite 1H resonances relative to tumor water, with total choline decreasing the most and lactate the least; and (iii) acute tumor blood flow reduction induced by administration of hydralazine results in doubling of the lactate intensity relative to water.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

Spatial heterogeneity of the metabolic response of RIF‐1 tumors to a vasoactive agent evaluated in vivo by one‐dimensional ³¹P chemical‐shift imaging

Cited by 4 publications

References 14 publications

Heat-induced changes in intracellular Na⁺, pH and bioenergetic status in superfused RIF-1 tumour cells determined by²³Na and³¹P magnetic resonance spectroscopy

Heat-induced changes in intracellular Na⁺, pH and bioenergetic status in superfused RIF-1 tumour cells determined by²³Na and³¹P magnetic resonance spectroscopy

Measuring Oxygenation In Vivo with MRS/MRI—From Gas Exchange to the Cell

¹H NMR spectroscopy of subcutaneous tumors in mice: Preliminary studies of effects of growth, chemotherapy and blood flow reduction

Contact Info

Product

Resources

About

Spatial heterogeneity of the metabolic response of RIF‐1 tumors to a vasoactive agent evaluated in vivo by one‐dimensional 31P chemical‐shift imaging

Cited by 4 publications

References 14 publications

Heat-induced changes in intracellular Na+, pH and bioenergetic status in superfused RIF-1 tumour cells determined by23Na and31P magnetic resonance spectroscopy

Heat-induced changes in intracellular Na+, pH and bioenergetic status in superfused RIF-1 tumour cells determined by23Na and31P magnetic resonance spectroscopy

Measuring Oxygenation In Vivo with MRS/MRI—From Gas Exchange to the Cell

1H NMR spectroscopy of subcutaneous tumors in mice: Preliminary studies of effects of growth, chemotherapy and blood flow reduction

Contact Info

Product

Resources

About

Spatial heterogeneity of the metabolic response of RIF‐1 tumors to a vasoactive agent evaluated in vivo by one‐dimensional ³¹P chemical‐shift imaging

Heat-induced changes in intracellular Na⁺, pH and bioenergetic status in superfused RIF-1 tumour cells determined by²³Na and³¹P magnetic resonance spectroscopy

Heat-induced changes in intracellular Na⁺, pH and bioenergetic status in superfused RIF-1 tumour cells determined by²³Na and³¹P magnetic resonance spectroscopy

¹H NMR spectroscopy of subcutaneous tumors in mice: Preliminary studies of effects of growth, chemotherapy and blood flow reduction