Spatial Genetic Structure of Coffee-Associated <i>Xylella fastidiosa</i> Populations Indicates that Cross Infection Does Not Occur with Sympatric Citrus Orchards

Francisco, C. L.; Ceresini, Paulo Cézar; Almeida, Rodrigo P. P.; Coletta-Filho, Helvécio Della

doi:10.1094/phyto-08-16-0300-r

Cited by 19 publications

(24 citation statements)

References 52 publications

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“…However, coffee-and citrus-infecting X. fastidiosa in Brazil, for example, are host specific and phylogenetically distinct (3), even though these crops and respective diseases occur sympatrically and share insect vectors. In fact, when adjacent citrus and coffee orchards were surveyed, all isolates were genetically split into coffee or citrus groups, indicating strong biological isolation (56). In summary, although HR has been frequently associated with X. fastidiosa host jumps and new diseases, even frequent gene flow among sympatric and closely related clades of X. fastidiosa may not be enough to break down biological barriers associated with host colonization.…”

Section: Support For Homologous Recombination On Host Adaptationmentioning

confidence: 98%

Xylella fastidiosa: Insights into an Emerging Plant Pathogen

Sicard

Zeilinger

Vanhove

et al. 2018

Annu. Rev. Phytopathol.

181

157

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The bacterium Xylella fastidiosa re-emerged as a plant pathogen of global importance in 2013 when it was first associated with an olive tree disease epidemic in Italy. The current threat to Europe and the Mediterranean basin, as well as other world regions, has increased as multiple X. fastidiosa genotypes have now been detected in Italy, France, and Spain. Although X. fastidiosa has been studied in the Americas for more than a century, there are no therapeutic solutions to suppress disease development in infected plants. Furthermore, because X. fastidiosa is an obligatory plant and insect vector colonizer, the epidemiology and dynamics of each pathosystem are distinct. They depend on the ecological interplay of plant, pathogen, and vector and on how interactions are affected by biotic and abiotic factors, including anthropogenic activities and policy decisions. Our goal with this review is to stimulate discussion and novel research by contextualizing available knowledge on X. fastidiosa and how it may be applicable to emerging diseases.

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Section: Support For Homologous Recombination On Host Adaptationmentioning

confidence: 98%

Xylella fastidiosa: Insights into an Emerging Plant Pathogen

Sicard

Zeilinger

Vanhove

et al. 2018

Annu. Rev. Phytopathol.

181

157

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“…pauca (e.g., see references 7 and 26). Furthermore, experimental research has shown host pathogen specificity within subspecies, which is indicative of ecological isolation of specific clades within the different subspecies, and the presence of gene flow between otherwise divergent populations (26,27). Therefore, while recombination is frequently observed in X. fastidiosa populations, it has so far not been analyzed at the genome level with representative phylogenetic coverage of currently known X. fastidiosa diversity.…”

mentioning

confidence: 99%

Genomic Diversity and Recombination among Xylella fastidiosa Subspecies

Vanhove

Retchless

Sicard

et al. 2019

Appl Environ Microbiol

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103

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Xylella fastidiosa is an economically important bacterial plant pathogen. With insights gained from 72 genomes, this study investigated differences among the three main subspecies, which have allopatric origins: X. fastidiosa subsp. fastidiosa, multiplex, and pauca. The origin of recombinogenic X. fastidiosa subsp. morus and sandyi was also assessed. The evolutionary rate of the 622 genes of the species core genome was estimated at the scale of an X. fastidiosa subsp. pauca subclade (7.62 ϫ 10 Ϫ7 substitutions per site per year), which was subsequently used to estimate divergence time for the subspecies and introduction events. The study characterized genes present in the accessory genome of each of the three subspecies and investigated the core genome to detect genes potentially under positive selection. Recombination is recognized to be the major driver of diversity in X. fastidiosa, potentially facilitating shifts to novel plant hosts. The relative effect of recombination in comparison to point mutation was calculated (r/m ϭ 2.259). Evidence of recombination was uncovered in the core genome alignment; X. fastidiosa subsp. fastidiosa in the United States was less prone to recombination, with an average of 3.22 of the 622 core genes identified as recombining regions, whereas a specific clade of X. fastidiosa subsp. multiplex was found to have on average 9.60 recombining genes, 93.2% of which originated from X. fastidiosa subsp. fastidiosa. Interestingly, for X. fastidiosa subsp. morus, which was initially thought to be the outcome of genome-wide recombination between X. fastidiosa subsp. fastidiosa and X. fastidiosa subsp. multiplex, intersubspecies homologous recombination levels reached 15.30% in the core genome. Finally, there is evidence of X. fastidiosa subsp. pauca strains from citrus containing genetic elements acquired from strains infecting coffee plants as well as genetic elements from both X. fastidiosa subsp. fastidiosa and X. fastidiosa subsp. multiplex. In summary, our data provide new insights into the evolution and epidemiology of this plant pathogen. IMPORTANCE Xylella fastidiosa is an important vector-borne plant pathogen. We used a set of 72 genomes that constitutes the largest assembled data set for this bacterial species so far to investigate genetic relationships and the impact of recombination on phylogenetic clades and to compare genome content at the subspecies level, and we used a molecular dating approach to infer the evolutionary rate of X. fastidiosa. The results demonstrate that recombination is important in shaping the genomes of X. fastidiosa and that each of the main subspecies is under different selective pressures. We hope insights from this study will improve our understanding of X. fastidiosa evolution and biology.

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“…Diseases on different host species caused by strains of subspecies pauca have been reported to be sympatric. For instance, citrus variegated chlorosis (CVC), CLS and olive leaf scorch are all present in some Brazilian states (Coletta-Filho et al, 2016;Francisco et al, 2017). Although there appears to be some degree of host specialization within the subspecies pauca (i.e.…”

Section: Taxonomy Host Range and Genetic Diversitymentioning

confidence: 99%

“…Although there appears to be some degree of host specialization within the subspecies pauca (i.e. strain specificity for either citrus or coffee; Prado et al, 2008), cross-infection has been reported (Li et al, 2001;Francisco et al, 2017). In addition, some strains that cause CVC and CLS can also cause disease symptoms in several commercial grapevine cultivars (Li et al, 2002).…”

Section: Taxonomy Host Range and Genetic Diversitymentioning

confidence: 99%

Coffee bacterial diseases: a plethora of scientific opportunities

Badel

Zambolim

2019

Plant Pathology

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Coffee is a very important crop for several tropical countries across different continents. The diseases bacterial halo blight (BHB), bacterial leaf spot (BLS), bacterial leaf blight (BLB) and coffee leaf scorch (CLS), caused by the bacterial pathogens Pseudomonas syringae pv. garcae (Psgc), P. syringae pv. tabaci (Psta), Pseudomonas cichorii (Pch) and Xylella fastidiosa subsp. pauca (Xfp), respectively, cause significant reductions in coffee production, although other minor bacterial diseases have also been reported in some countries. Little research progress has been made on aspects that are relevant for control and management of these diseases. In all cases, there is an urgent need to develop rapid and more reliable methods for early detection of the pathogens in order to minimize their negative impact on coffee production. Because of the high rate of intra‐ and intersubspecific recombination occurring in X. fastidiosa, a permanent revision of the detection methods is necessary. Greater efforts should be made to understand the genetic and virulence diversity of Psgc, Psta and Pch populations. Early studies reported the identification of potential sources of resistance against Psgc and Psta, but, to date, no resistance gene has been isolated. Little effort has been made to understand the biology and molecular mechanisms underlying the interaction between Coffea spp. and these pathogenic bacteria. This review discusses the recent progress on the molecular mechanisms used by these bacteria to cause diseases on other plant species, in order to provide a guideline for the establishment of future research programmes.

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Spatial Genetic Structure of Coffee-Associated Xylella fastidiosa Populations Indicates that Cross Infection Does Not Occur with Sympatric Citrus Orchards

Abstract: TitleSpatial genetic structure of coffee-associated xylella fastidiosa populations indicates that cross infection does not occur with sympatric citrus orchards Permalink https://escholarship.org/uc/item/07k907bt Journal Phytopathology, 107(4)

Cited by 19 publications

References 52 publications

Xylella fastidiosa: Insights into an Emerging Plant Pathogen

Xylella fastidiosa: Insights into an Emerging Plant Pathogen

Genomic Diversity and Recombination among Xylella fastidiosa Subspecies

Coffee bacterial diseases: a plethora of scientific opportunities

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