Spatial division of phosphoenolpyruvate carboxylase and nitrate reductase activity and its regulation by cytokinins in CAM-induced leaves of Guzmania monostachia (Bromeliaceae)

Pereira, Paula Natália; Purgatto, Eduardo; Mercier, Helenice

doi:10.1016/j.jplph.2013.03.005

Cited by 23 publications

(34 citation statements)

References 31 publications

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“…Four hours before the beginning of the NR activity assay, KNO 3 (6 mol L À1 ) was added to each sample because nitrate is the substrate of NR. Nitrate reduction was measured using in vivo assay (Pereira et al, 2013). Briefly, 1 g of fresh lettuces was weighed and incubated in 6 mL of 0.1 mol L À1 phosphate buffer (pH 7.5) containing 3% propanol and 0.1 mol L À1 KNO 3 .…”

Section: Determination Of Nitrate Reductase Activitymentioning

confidence: 99%

Reduction of nitrite levels in fresh lettuces with aqueous chlorine dioxide treatment

Hao

Wan

et al. 2014

Int J of Food Sci Tech

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Summary Leafy vegetables are the major source of nitrite intake in the human diet, and technological processing to control the nitrite levels is necessary to the harvested vegetables destined for consumption. In this work, the effect of aqueous chlorine dioxide (ClO2) treatment on the nitrite levels in fresh lettuces during storage was studied. The results showed the appropriate aqueous ClO2 solutions treatment (30 and 45 mg L−1, 10 min) could reduce the nitrite levels or retard the peak occurrence in fresh lettuces during storage. The reduction of nitrite levels in fresh lettuces with appropriate ClO2 treatment can be attributed to two aspects including the inactivation of nitrate reductase directly and the reduction of the bacterial populations. This study will provide a useful method to reduce the nitrite levels in fresh lettuces destined for short storage followed by consumption.

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Section: Determination Of Nitrate Reductase Activitymentioning

confidence: 99%

Reduction of nitrite levels in fresh lettuces with aqueous chlorine dioxide treatment

Hao

Wan

et al. 2014

Int J of Food Sci Tech

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“…Nitrate reduction was measured using in vivo assay (36). Briefly, 1 g of fresh spinach was weighed and incubated in 6 ml of 0.1 mol/liter phosphate buffer (pH 7.5) containing 3% propanol and 0.1 mol/liter K N 0 3.…”

mentioning

confidence: 99%

Effect of Electrolyzed Oxidizing Water Treatment on the Reduction of Nitrite Levels in Fresh Spinach during Storage

Hao¹,

Li²,

Wan³

et al. 2015

Journal of Food Protection

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Leafy vegetables are the major source of nitrite intake in the human diet, and technological processing to control nitrite levels in harvested vegetables is necessary. In the current work, the effect of electrolyzed oxidizing water (EOW) on the nitrite and nitrate levels in fresh spinach during storage was studied. EOW treatment, including slightly acidic electrolyzed water and acidic electrolyzed water, was found to effectively reduce nitrite levels in fresh spinach during storage; levels in the late period were 30 to 40% lower than that of the control. However, the nitrate levels in fresh spinach during storage were not influenced by EOW treatment. The reduction of nitrite levels in EOW-treated fresh spinach during storage can be attributed to the inactivation of nitrate reductase directly and to the reduction of bacterial populations. Our results suggest that treatment with slightly acidic electrolyzed water may be a better choice to control nitrite levels in fresh vegetables during storage. This study provided a useful method to reduce nitrite levels in fresh spinach.

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“…Um promissor modelo de estudo para esse tipo de transição metabólica é a espécie Guzmania monostachia (Bromeliaceae), uma bromélia epífita que pode alternar, em diferentes condições ambientais, entre os metabolismos C 3 e CAM (Freschi et al, 2010b;Pereira et al, 2013). Resultados recentes obtidos em nosso laboratório mostraram diferenças significativas quanto à acidez noturna, atividade da fosfoenolpiruvato carboxilase (PEPC) e da enzima malato desidrogenase (MDH) nas porções basal, mediana e apical das folhas de Guzmania monostachia.…”

Section: O Metabolismo áCido Das Crassuláceas (Cam)unclassified

Influência das fontes de N e do déficit hídrico sobre a expressão de aquaporinas e/ou transporte de ácidos orgânicos em plantas CAM

Pereira¹

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Spatial division of phosphoenolpyruvate carboxylase and nitrate reductase activity and its regulation by cytokinins in CAM-induced leaves of Guzmania monostachia (Bromeliaceae)

Cited by 23 publications

References 31 publications

Reduction of nitrite levels in fresh lettuces with aqueous chlorine dioxide treatment

Reduction of nitrite levels in fresh lettuces with aqueous chlorine dioxide treatment

Effect of Electrolyzed Oxidizing Water Treatment on the Reduction of Nitrite Levels in Fresh Spinach during Storage

Influência das fontes de N e do déficit hídrico sobre a expressão de aquaporinas e/ou transporte de ácidos orgânicos em plantas CAM

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