Spatial distribution of progesterone in bovine uterus in relation to corpus luteum location and function

Cerbito, W.A.; Quero, F.V.; Balagapo, C.R.; Miyazawa, Keiji; Sato, Kunitada

doi:10.1016/0093-691x(94)90831-3

Cited by 10 publications

(4 citation statements)

References 18 publications

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“…In fact, a study by Cerbito et al . [ 5 ] showed that bovine endometrial tissue P4 concentrations in the horn ipsilateral to the ovary containing a CL are higher than that in the horn contralateral to the ovary containing the CL. Therefore, we hypothesized that the difference in the intrauterine environment between the ipsilateral and contralateral horns may be regulated by P4 and its receptors in the endometrial tissue of the ipsilateral and contralateral horns.…”

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confidence: 99%

Differences in progesterone concentrations and mRNA expressions of progesterone receptors in bovine endometrial tissue between the uterine horns ipsilateral and contralateral to the corpus luteum

Takahashi

Haneda

Kayano

et al. 2016

The Journal of Veterinary Medical Science

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Because the establishment of pregnancy begins at the uterine horn ipsilateral to the corpus luteum (ipsi-horn) in cattle, levels of progesterone (P4) and receptor expression in the endometrial tissue, which regulate the intrauterine environment for embryo development, may differ between the ipsi-horn and the uterine horn contralateral to corpus luteum (contra-horn). The aim of the present study was to determine the endometrial tissue P4 concentrations and nuclear progesterone receptor (PGR), progesterone receptor membrane component 1 (PGRMC1) and PGRMC2 mRNA expressions in the cranial and middle parts of the uterine horns during the luteal phase. The results showed higher endometrial tissue P4 concentrations in the cranial part of the ipsi-horn than in that of the contra-horn (P<0.01); however, no change in the endometrial tissue P4 concentrations was evident during the luteal phase. The PGR mRNA expression was higher during the early luteal phase (P<0.05), but no differences between the horns were evident. However, PGRMC1 mRNA expression during the early luteal phase was higher in the cranial part of the ipsi-horn than in that of the contra-horn (P<0.05). In the middle part, there were no changes in the endometrial tissue P4 concentrations and P4 receptor expressions during the luteal phase. In conclusion, the differences in dynamics of endometrial tissue P4 concentrations and P4 receptor expressions between the uterine horns ipsilateral and contralateral to the ovary containing a corpus luteum may cause differences in the intrauterine environment for both the ipsi- and contra-horns.

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confidence: 99%

Differences in progesterone concentrations and mRNA expressions of progesterone receptors in bovine endometrial tissue between the uterine horns ipsilateral and contralateral to the corpus luteum

Takahashi

Haneda

Kayano

et al. 2016

The Journal of Veterinary Medical Science

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“…Our earlier finding [16] that a substantial improvement in pregnancy per AI occurred without a corresponding increase in peripheral blood progesterone concentrations raised the possibility that pLH-induced CL produces more P 4 locally. Uterine tissue P 4 concentrations are driven by the P 4 content of CL [29], and P 4 distributions in the bovine uterus, broad ligament and uterine arteries are preferentially increased on the side ipsilateral to the ovary bearing the CL [30]. A P 4 gradient within these tissues has been reported [30], with significantly greater concentrations of P 4 in tissues closest to the ovary bearing the CL (e.g., mesosalpinx, anterior uterine horn, and branches of the uterine artery supplying that area) than in more distal tissues or the main uterine artery.…”

Section: Discussionmentioning

confidence: 99%

Morphologic, Steroidogenic, and Transcriptomic Assessment of the Corpus Luteum in Holstein Cows after Spontaneous or Hormone-Induced Ovulation

Ponce-Barajas

Colazo

Behrouzi

et al. 2023

Animals

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There is evidence that replacing the gonadotropin-releasing hormone (GnRH) with porcine luteinizing hormone (pLH) to synchronize ovulation prior to artificial insemination (AI) increased pregnancy per AI in dairy cows without affecting blood progesterone (P4) concentrations. Whether morphologic, steroidogenic, and transcriptomic differences exist among corpora lutea (CL) formed after ovulation induced by GnRH and pLH is unclear. Our main objective, therefore, was to compare CL characteristics between GnRH- and pLH-induced CL. In 24 non-lactating Holstein cows, ovulations were spontaneous (Spont-Ov) or induced with 100 µg GnRH, 25 mg pLH, or 1 mg estradiol benzoate (EB), with CL excised 12 d after ovulation. In pLH- versus GnRH-treated cows, the duration of elevated LH (above baseline) was prolonged (10 versus 6 h, respectively, p < 0.01), but CL dimensions, pixel intensity of CL images, proportions of steroidogenic and non-steroidogenic luteal cells, and mean plasma LH did not significantly differ. Post-ovulation mean plasma P4 (ng/mL) did not differ among Spont-Ov (3.0) pLH (3.1) or GnRH (3.0) cows but were lower in EB cows (2.0). In vitro P4 concentration was greater in luteal explants of pLH-treated cows than in all other groups (combined means, 16.0 vs. 12.3 µg/mL, p < 0.02). Relative abundance of mRNA for oxytocin receptor (OXTR) was 2-fold higher (p < 0.01) in CL of pLH vs. GnRH cows and highest in Spont-Ov CL. In summary, pLH-treated cows had a longer LH peak, and greatest luteal tissue concentrations and in vitro production of P4. We inferred that increased P4 concentrations at the ovarian–uterine level in pLH-treated cows could have promoted embryo development and increased pregnancy per AI.

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“…Hasler and others (1980) reported similar findings; embryo transfer recipients with serum progesterone concentrations of 0.13 ng/ml on day 5 maintained a pregnancy. Peripheral progesterone concentrations are susceptible to daily fluctuations (Lukaszewska and Hansel 1980, Cerbito and others 1994) and may not be as relevant as local concentration and receptor occupancy in maintaining pregnancy (Okumu and others 2010). …”

Section: Discussionmentioning

confidence: 99%

Identification of inadequate maternal progesterone concentrations in nulliparous dairy heifers and treatment with human chorionic gonadotrophin

King¹,

Dobson

Royal

et al. 2013

Veterinary Record

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Plasma progesterone concentrations were estimated for 381 nulliparous dairy heifers from eight farms on days 4-6 after insemination. Heifers were synchronised using two treatments of prostaglandin F2α (PGF2α) 11 days apart. Alternate heifers were treated with 1500 IU human chorionic gonadotrophin (hCG) at the time of sampling, and the pregnancy status assessed ~35 days after insemination. Heifers with no corpus luteum (CL; n-30) at the second PGF2α had lower progesterone concentrations (P<0.001) and pregnancy rates (PR; P=0.001) compared with heifers with CL (n=351, defined as cyclic). The risk of no CL increased in heifers <15 months of age (OR=7.5, P<0.001) and with body condition score <2.5 (OR=4.5, P=0.001). Those with no CL were removed from further analysis. The 20 per cent cyclic control heifers with progesterone concentrations ≤2 ng/ml on days 4-6 had lower PRs compared with those with higher concentrations (42 per cent vs 64 per cent, OR=0.3, P=0.01). There was no overall effect of hCG on PR compared with controls (51 per cent vs 60 per cent, P=0.10). However, heifers with high progesterone on days 4-6 (>2 ng/ml) had a lower PR after treatment (51 per cent vs 64 per cent, OR=0.6, P=0.02). Heifers inseminated with sexed (n=18) compared with conventional (n=333) semen had a decreased PR (33 per cent vs 57 per cent, OR=0.2, P=0.01). This study demonstrates that higher plasma progesterone concentrations as early as days 4-6 after insemination have a positive effect on first insemination PR, but there was no beneficial effect of hCG treatment on these days in dairy heifers.

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Spatial distribution of progesterone in bovine uterus in relation to corpus luteum location and function

Cited by 10 publications

References 18 publications

Differences in progesterone concentrations and mRNA expressions of progesterone receptors in bovine endometrial tissue between the uterine horns ipsilateral and contralateral to the corpus luteum

Differences in progesterone concentrations and mRNA expressions of progesterone receptors in bovine endometrial tissue between the uterine horns ipsilateral and contralateral to the corpus luteum

Morphologic, Steroidogenic, and Transcriptomic Assessment of the Corpus Luteum in Holstein Cows after Spontaneous or Hormone-Induced Ovulation

Identification of inadequate maternal progesterone concentrations in nulliparous dairy heifers and treatment with human chorionic gonadotrophin

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