Spatial distribution of osteoblast activating peptide in the rat stomach

Noreldin, Ahmed E.; Sogabe, Maina; Yamano, Yoshiaki; Uehara, Masato; Mahdy, Mohamed A. A.; Elnasharty, Mohamed; Sayed-Ahmed, Ahmed; Warita, Katsuhiko; Hosaka, Yoshinao Z.

doi:10.1016/j.acthis.2015.12.001

Cited by 10 publications

(4 citation statements)

References 45 publications

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“…Table S2 lists antibodies, sources, dilutions, and methods for retrieval of the antigen. The immunohistochemical reactions in the pancreatic and kidney sections were investigated according to the method described by Noreldin et al [ 53 ] and Noreldin et al [ 54 ]. Sections as prepared for histopathological assessment were deparaffinized by xylene, rehydrated in graded alcohols, and washed by distilled water.…”

Section: Methodsmentioning

confidence: 99%

Quercetin Attenuates Pancreatic and Renal D-Galactose-Induced Aging-Related Oxidative Alterations in Rats

El‐Far

Lebda

Noreldin

et al. 2020

IJMS

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Aging is an oxidative stress-associated process that progresses with age. Our aim is to delay or attenuate these oxidative alterations and to keep individuals healthy as they age using natural compounds supplementation. Therefore, we conducted the present study to investigate the protective potentials of quercetin against D-galactose (D-gal)-associated oxidative alterations that were induced experimentally in male Wistar rats. Forty-five rats were randomly allocated into five groups of nine rats each. The groups were a control group that was reared on a basal diet and injected subcutaneously with 120 mg D-gal dissolved in physiological saline solution (0.9% NaCl) per kg body weight daily and quercetin-treated groups that received the same basal diet and subcutaneous daily D-gal injections were supplemented orally with 25, 50, and 100 mg of quercetin per kg body weight for 42 days. Pancreatic and renal samples were subjected to histopathological, immunohistochemical, and relative mRNA expression assessments. Aging (p53, p21, IL-6, and IL-8), apoptotic (Bax, CASP-3, and caspase-3 protein), proliferative (Ki67 protein), antiapoptotic (Bcl2 and Bcl2 protein), inflammatory (NF-κB, IL-1β, and TNF-α), antioxidant (SOD1), and functional markers (GCLC and GCLM genes and insulin, glucagon, and podocin proteins) were determined to evaluate the oxidative alterations induced by D-gal and the protective role of quercetin. D-gal caused oxidative alterations of the pancreas and kidneys observed via upregulations of aging, apoptotic, and inflammatory markers and downregulated the antiapoptotic, proliferative, antioxidant, and functional markers. Quercetin potentially attenuated these aging-related oxidative alterations in a dose-dependent manner. Finally, we can conclude that quercetin supplementation is considered as a promising natural protective compound that could be used to delay the aging process and to maintain human health.

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Section: Methodsmentioning

confidence: 99%

Quercetin Attenuates Pancreatic and Renal D-Galactose-Induced Aging-Related Oxidative Alterations in Rats

El‐Far

Lebda

Noreldin

et al. 2020

IJMS

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“…Histopathological [58], immunohistochemistry [59,60], and RT-PCR assessments of brain and heart samples were done as described in our previous study, El-Far et al [61]. Antibodies used in the immunohistochemical assay are listed in Table 2, and the primer's sequence of tested genes is listed in Table 3.…”

Section: Biochemical Histopathological Immunohistochemical and Reverse Transcription-polymerase Chain Reaction (Rt-pcr) Assessmentsmentioning

confidence: 99%

Thymoquinone and Curcumin Defeat Aging-Associated Oxidative Alterations Induced by D-Galactose in Rats’ Brain and Heart

El‐Far

Elewa

Abdelfattah

et al. 2021

IJMS

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D-galactose (D-gal) administration causes oxidative disorder and is widely utilized in aging animal models. Therefore, we subcutaneously injected D-gal at 200 mg/kg BW dose to assess the potential preventive effect of thymoquinone (TQ) and curcumin (Cur) against the oxidative alterations induced by D-gal. Other than the control, vehicle, and D-gal groups, the TQ and Cur treated groups were orally supplemented at 20 mg/kg BW of each alone or combined. TQ and Cur effectively suppressed the oxidative alterations induced by D-gal in brain and heart tissues. The TQ and Cur combination significantly decreased the elevated necrosis in the brain and heart by D-gal. It significantly reduced brain caspase 3, calbindin, and calcium-binding adapter molecule 1 (IBA1), heart caspase 3, and BCL2. Expression of mRNA of the brain and heart TP53, p21, Bax, and CASP-3 were significantly downregulated in the TQ and Cur combination group along with upregulation of BCL2 in comparison with the D-gal group. Data suggested that the TQ and Cur combination is a promising approach in aging prevention.

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“…The existence of these two populations of parietal cells has been supported by six approaches: (1) young parietal cells have more developed canaliculi and rough endoplasmic reticulum than old parietal cells, which show signs of degeneration 41 ; (2) young parietal cells, but not base parietal cells, are activated by food 42 ; (3) H + /K + ATPase activity found in young parietal cells is absent from old parietal cells 43 ; (4) upper parietal cells respond much more efficiently than basal parietal cells to acid inhibitors and secretagogues 44 ; (5) the level of H + /K + ATPase β-subunit mRNA is high in parietal cells near the isthmus but is low in distant cells 44 ; and (6) there is a different production of the osteoblast activating peptide by parietal cells of the neck and base regions. 45 Here, we have shown a seventh approach to support the existence of two subpopulations of parietal cells. Our findings indicated that parietal cells express different glycoconjugates depending on their location in the upper or lower region of the gland: Cells in the upper region of the gland, near the isthmus, express different carbohydrates than cells in the base of the gland.…”

Section: Discussionmentioning

confidence: 77%

Characterization by Lectin Histochemistry of Two Subpopulations of Parietal Cells in the Rat Gastric Glands

Gomez-Santos

Alonso

Díaz‐Flores

et al. 2017

J Histochem Cytochem.

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Parietal cells undergo a differentiation process while they move from the isthmus toward the pits and the base region of the gastric gland. The aim of this work was to analyze the rat gastric glands by lectin histochemistry to show the glycans expressed by upper (young) and lower (old) parietal cells. We used lectins recognizing the most frequent sugar moieties in mammals. Each lectin was assayed alone and in combination with several deglycosylation pretreatments: (1) β-elimination, which removes O-linked oligosaccharides; (2) incubation with Peptide-N-glycosidase F, to remove N-linked glycans; (3) acid hydrolysis, which removes terminal sialic acid moieties; (4) methylation-saponification, to remove sulfate groups from sugar residues; and (5) glucose oxidase, a technique carried out with the lectin concanavalin A to convert glucose into gluconic acid. The lectins from Helix pomatia, Dolichos biflorus (DBA), Glycine max (soybean), Maclura pomifera, Arachis hypogaea (peanut), Bandeiraea simplicifolia (lectin I-B), and Datura stramonium showed a different glycan expression in the parietal cells throughout the gastric gland. This difference supports that parietal cells undergo a maturation/degeneration process while the cells descend along the gland. The role of DBA as a marker of parietal cells previously reported should be taken with caution because these cells showed different reactivity for the lectin, ranging from negative to strong labeling.

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Spatial distribution of osteoblast activating peptide in the rat stomach

Cited by 10 publications

References 45 publications

Quercetin Attenuates Pancreatic and Renal D-Galactose-Induced Aging-Related Oxidative Alterations in Rats

Quercetin Attenuates Pancreatic and Renal D-Galactose-Induced Aging-Related Oxidative Alterations in Rats

Thymoquinone and Curcumin Defeat Aging-Associated Oxidative Alterations Induced by D-Galactose in Rats’ Brain and Heart

Characterization by Lectin Histochemistry of Two Subpopulations of Parietal Cells in the Rat Gastric Glands

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