Spatial distribution of enamel proteins and fibronectin at early stages of rat incisor tooth formation

The zebrafish vitronectin receptor: Characterization of integrin αV and β3 expression patterns in early vertebrate development

“…Moreover, there are two reported zebrafish fibronectin1 mutants, natter (nat) (Trinh and Stainier, 2004), and kt259 (Koshida et al, 2005); consequently, it would be interesting to determine if they exhibit pharyngeal dentition defects. Although fibronectin was proposed to function in ameloblast and odontoblast differentiation in murine incisor tooth formation (Ruch et al, 1995;Sawada and Nanci, 1995;Tabata et al, 2003), our preliminary WISH studies with fn1 indicate that expression is absent in the fifth ceratobranchials until 4 dpf (personal observations), suggesting that FN1 may not have similar function in zebrafish pharyngeal dentitions. Another possible candidate ligand for ␣V␤3.1 is vitronectin, which is produced primarily in the liver and secreted into the blood (Holmes, 1967).…”

“…Double ISH samples: (D) ␣V (orange-red), ␤3.1 (dark purple); (G) ␣V (orange-red), pitx2 (dark purple); (J) ␣V (orange-red), and dlx2b (dark purple); (K) ␣V (dark purple), and cx43 (orange-red). Scale bars ϭ 20 m. murine tooth development (e.g., fibronectin, type IV collagen, laminin, and entactin) (Thesleff and Hurmerinta, 1981;Thesleff et al, 1991;Sawada and Nanci, 1995;Salmivirta et al, 1997), there is only one orthologous fibronectin1 (fn1) gene in zebrafish that has been identified so far. Moreover, there are two reported zebrafish fibronectin1 mutants, natter (nat) (Trinh and Stainier, 2004), and kt259 (Koshida et al, 2005); consequently, it would be interesting to determine if they exhibit pharyngeal dentition defects.…”

Endocytotic functions of ameloblasts and odontoblasts: Immunocytochemical and tracer studies on the uptake of plasma proteins

“…Hence, the production of enamel proteins starts much sooner than expected, during the presecretory stage (Slavkin et al, 1988). These first proteins are not associated with mineral deposition or appositional growth of the enamel layer and consequently may be involved, by themselves or synergistically with other proteins, in the differentiation and/or formative events taking place at the ameloblast-odontoblast interface during the early stages of tooth development (Sawada and Nanci, 1995).…”

Specific Amelogenin Gene Splice Products Have Signaling Effects on Cells in Culture and in Implants in Vivo

“…Others (60 -62) have shown by immunostaining that the mantle dentin contains amelogenin-related peptides. Sawada and Nanci (62) postulated that low molecular size amelogenin degradation products diffuse through the basement membrane separating preameloblasts and preodontoblasts and become trapped between odontoblasts in the forming dentin. Karg et al (63) found amelogenin immunostaining in developing hamster teeth in the early predentin and adjacent partially polarized preameloblasts before any overt deposition of enamel.…”