Spatial distribution and karyotypic composition of Anopheles mosquitoes (Diptera, Culicidae) in the environs of Teletskoye Lake

Перевозкин, В. П.; Принцева, А. А.; Бондарчук, С. С.; Gordeev, М. I.

doi:10.1134/s1067413612030113

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“…The region of Medvezh'egorsk and Segezha is a plateau with an altitude of 300 m. Our results agree well with the data on the mosquito species distribution in the region of the Teletskoe Lake (Altai) [12]. In this region of the Altai Mountains, A. beklem ishevi dominates in the northern part, which is also a plateau (altitude approximately 400 m) with a rela tively low annual temperature and a higher humidity.…”

Section: Resultssupporting

confidence: 92%

Inversion polymorphism and ecological specialization of malaria mosquitoes (Diptera, Culicidae) in Karelia

et al. 2012

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The species composition and inversion polymorphism were studied in malaria mosquito larva hemipopulations of Karelia. Three malaria mosquito species-Anopheles messeae, A. beklemishevi, and A. maculipennis-were found in the region. The northern boundary of their range is at 65°N. The greatest species diversity was observed in biotopes of the central region. Within species chromosome polymorphism was observed in larva populations of all three species. For A. messeae, maximum karyotype diversity indices were established for the southern and northern regions of Karelia.

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Section: Resultssupporting

confidence: 92%

Inversion polymorphism and ecological specialization of malaria mosquitoes (Diptera, Culicidae) in Karelia

et al. 2012

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A standard photomap of ovarian nurse cell chromosomes and inversion polymorphism in Anopheles beklemishevi

et al. 2018

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BackgroundAnopheles beklemishevi is a member of the Maculipennis group of malaria mosquitoes that has the most northern distribution among other members of the group. Although a cytogenetic map for the larval salivary gland chromosomes of this species has been developed, a high-quality standard cytogenetic photomap that enables genomics and population genetics studies of this mosquito at the adult stage is still lacking.MethodsIn this study, a cytogenetic map for the polytene chromosomes of An. beklemishevi from ovarian nurse cells was developed using high-resolution digital imaging from field collected mosquitoes. PCR-amplified DNA probes for fluorescence in situ hybridization (FISH) were designed based on the genome of An. atroparvus. The DNA probe obtained by microdissection procedures from the breakpoint region was labelled in a DOP-PCR reaction. Population analysis was performed on 371 specimens collected in 18 locations.ResultsWe report the development of a high-quality standard photomap for the polytene chromosomes from ovarian nurse cells of An. beklemishevi. To confirm the suitability of the map for physical mapping, several PCR-amplified probes were mapped to the chromosomes of An. beklemishevi using FISH. In addition, we identified and mapped DNA probes to flanking regions of the breakpoints of two inversions on chromosome X of this species. Inversion polymorphism was determined in 13 geographically distant populations of An. beklemishevi. Four polymorphic inversions were detected. The positions of common chromosomal inversions were indicated on the map.ConclusionsThe study constructed a standard photomap for ovarian nurse cell chromosomes of An. beklemishevi and tested its suitability for physical genome mapping and population studies. Cytogenetic analysis determined inversion polymorphism in natural populations of An. beklemishevi related to this species’ adaptation.Electronic supplementary materialThe online version of this article (10.1186/s13071-018-2657-3) contains supplementary material, which is available to authorized users.

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Spatial distribution and karyotypic composition of Anopheles mosquitoes (Diptera, Culicidae) in the environs of Teletskoye Lake

Cited by 2 publications

References 1 publication

Inversion polymorphism and ecological specialization of malaria mosquitoes (Diptera, Culicidae) in Karelia

Inversion polymorphism and ecological specialization of malaria mosquitoes (Diptera, Culicidae) in Karelia

A standard photomap of ovarian nurse cell chromosomes and inversion polymorphism in Anopheles beklemishevi

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