Spatial differences in soil microbial diversity caused by pH ‐driven organic phosphorus mineralization

Abstract

Microbial diversity response to abiotic and biotic factors provides a sensitive indicator for estimating the potential stability and degradation of soils in agro‐ecosystems. To determine the effects of pH on organic phosphorus mineralization and microbial diversity, phospholipid fatty acid analysis, quantitative polymerase chain reaction (qPCR), and multiple ecological analyses were performed. Significant correlations were found between phosphorus components and alkaline phosphatase, phytase, and pH, between phoD and phytase, between bpp and alkaline phosphatase. phoD and bpp gene abundance presented significant linear relationships with soil pH and microbial diversity. Abiotic and biotic factors explained 25.1% of the total variation in organic phosphorus‐mineralizing‐related gene abundance, and abiotic factors accounted for 13.2% of the total variation in microbial community composition. Soil pH was the determinant, accounting for 11.2 and 7.7% of the total variation in organic phosphorus‐mineralizing‐related gene abundance and microbial community composition, respectively. Our results emphasized that the phosphorus components, pH, and organic phosphorus‐mineralizing‐related gene abundance were responsible for organic phosphorus‐mineralizing‐related enzyme activity. To our knowledge, this is the first report that pH is a key factor in directly and indirectly determining organic phosphorus‐mineralizing‐related gene abundance, which in turn affects microbial diversity, on a large spatial scale. The differences in phosphorus components, enzyme activity, organic phosphorus‐mineralizing‐related gene abundance, microbial community composition and diversity caused by pH might explain crop yield reduction.

Phosphate-Solubilizing Bacterium Acinetobacter pittii gp-1 Affects Rhizosphere Bacterial Community to Alleviate Soil Phosphorus Limitation for Growth of Soybean (Glycine max)

“…The structural equation model reflected stronger interconnections among vegetation properties, soil physicochemical properties, P-cycling-related gene abundance, cell exudates, and bacterial community composition. This result is similar to our prior finding (Wan et al, 2021a). The cooccurrence network also showed that core taxa belonging to Acidobacteria, Chloroflexi, Gemmatimonadetes, and Proteobacteria presented significant effects on vegetation properties.…”

“…We scraped rhizosphere soils by using a brush. We measured soil physicochemical properties, including pH, total carbon, total nitrogen, and available P, based on standard methods (Wan et al, 2021a). Microbial biomass P was evaluated by chloroform fumigation extraction and was calculated as the difference between fumigated and non-fumigated subsamples and simultaneously revised for the incomplete recovery of a P spike (Roberts et al, 2013;Ragot et al, 2016).…”

Bridging Rare and Abundant Bacteria with Ecosystem Multifunctionality in Salinized Agricultural Soils: from Community Diversity to Environmental Adaptation

“…We determined soil physicochemical properties, including electrical conductivity (EC), pH, total carbon (TC), total nitrogen (TN), NH 4 + -N, NO 3 − -N, total phosphorus (TP), available phosphorus (AP), inorganic phosphorus (IP), organic phosphorus (OP), total potassium (TK), and available potassium (AK). Detailed descriptions of determination approaches were reported in previous studies ( 37 , 61 ).…”

Environmental adaptation is stronger for abundant rather than rare microorganisms in wetland soils from the Qinghai‐Tibet Plateau

“…Microorganisms in wetland soils are one of the largest world reservoirs of biodiversity and drive numerous ecological processes in terrestrial ecosystems (Louca et al, 2018; Shi et al, 2018; Wagg et al, 2014). Specifically, bacteria and fungi are responsible for the turnover and cycling of important elements, including carbon, nitrogen, and phosphorus (Ge et al, 2012; Luo et al, 2018; Wan, Hao, et al, 2021). In addition, soil microbial diversity (α‐ and β‐diversity) has been shown to be closely correlated with multiple ecosystem functions, such as primary production and climate regulation (Luo et al, 2018; Wagg et al, 2014).…”