Spatial control of membrane traffic in neuronal dendrites

Radler, Megan R.; Suber, Ayana; Spiliotis, Elias T.

doi:10.1016/j.mcn.2020.103492

Cited by 23 publications

(17 citation statements)

References 324 publications

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“…SEPT7 may similarly function in the capture and guidance of microtubule plus ends at the base of dendritic spines in hippocampal neurons and at cortical sites of neurite reformation in neural crest cells following mitosis [126][127][128] . Consistent with this possibility, SEPT2-SEPT6-SEPT7 filaments are more potent than actin in capturing microtubule plus ends in vitro 129 , and membrane septins (SEPT2-SEPT6-SEPT7-SEPT9) capture microtubules in cells infected with a Clostridium difficile toxin, which depolymerizes actin and induces microtubule-driven membrane protrusions 119 . Notably, septins are also required for the attachment of mitotic microtubules to the plasma membrane of the mother-bud neck cortex of S. cerevisiae 130,131 .…”

Section: Roles Of Microtubule-associated Septins In Cellular Morphogenesismentioning

confidence: 84%

Cellular functions of actin- and microtubule-associated septins

Spiliotis

Nakos

2021

Current Biology

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Section: Roles Of Microtubule-associated Septins In Cellular Morphogenesismentioning

confidence: 84%

Cellular functions of actin- and microtubule-associated septins

Spiliotis

Nakos

2021

Current Biology

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“…These morphological aspects can differentiate neurons between species and their functions ( DeFelipe, 2011 ). The pattern of synaptic organization of different pyramidal neurons is elaborated for each cortical area in the human brain, at the same time that is set at the level of each dendritic segment and the modulatory processes made by each spine ( Andersen et al, 2007 ; Bourne and Harris, 2009 ; Chen and Sabatini, 2012 ; Spruston et al, 2013 ; Brusco et al, 2014 ; Chen et al, 2014 ; Scholtens et al, 2014 ; Stewart et al, 2014 ; Dall’Oglio et al, 2015 ; Hayashi-Takagi et al, 2015 ; Palomero-Gallagher and Zilles, 2017 ; Radler et al, 2020 ).…”

Section: Integrating Pyramidal Morphology On Complex Network In the mentioning

confidence: 99%

The Subcortical-Allocortical- Neocortical continuum for the Emergence and Morphological Heterogeneity of Pyramidal Neurons in the Human Brain

Rasia‐Filho

Guerra

Vásquez

et al. 2021

Front. Synaptic Neurosci.

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Human cortical and subcortical areas integrate emotion, memory, and cognition when interpreting various environmental stimuli for the elaboration of complex, evolved social behaviors. Pyramidal neurons occur in developed phylogenetic areas advancing along with the allocortex to represent 70–85% of the neocortical gray matter. Here, we illustrate and discuss morphological features of heterogeneous spiny pyramidal neurons emerging from specific amygdaloid nuclei, in CA3 and CA1 hippocampal regions, and in neocortical layers II/III and V of the anterolateral temporal lobe in humans. Three-dimensional images of Golgi-impregnated neurons were obtained using an algorithm for the visualization of the cell body, dendritic length, branching pattern, and pleomorphic dendritic spines, which are specialized plastic postsynaptic units for most excitatory inputs. We demonstrate the emergence and development of human pyramidal neurons in the cortical and basomedial (but not the medial, MeA) nuclei of the amygdala with cells showing a triangular cell body shape, basal branched dendrites, and a short apical shaft with proximal ramifications as “pyramidal-like” neurons. Basomedial neurons also have a long and distally ramified apical dendrite not oriented to the pial surface. These neurons are at the beginning of the allocortex and the limbic lobe. “Pyramidal-like” to “classic” pyramidal neurons with laminar organization advance from the CA3 to the CA1 hippocampal regions. These cells have basal and apical dendrites with specific receptive synaptic domains and several spines. Neocortical pyramidal neurons in layers II/III and V display heterogeneous dendritic branching patterns adapted to the space available and the afferent inputs of each brain area. Dendritic spines vary in their distribution, density, shapes, and sizes (classified as stubby/wide, thin, mushroom-like, ramified, transitional forms, “atypical” or complex forms, such as thorny excrescences in the MeA and CA3 hippocampal region). Spines were found isolated or intermingled, with evident particularities (e.g., an extraordinary density in long, deep CA1 pyramidal neurons), and some showing a spinule. We describe spiny pyramidal neurons considerably improving the connectional and processing complexity of the brain circuits. On the other hand, these cells have some vulnerabilities, as found in neurodegenerative Alzheimer’s disease and in temporal lobe epilepsy.

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“…In addition to these roles, myosin Va has also been proposed to function in maintaining the polarized transport of dendrite‐selective vesicles 19,20 . Dendrite‐selective vesicles move in the somatodendritic domain but do not enter the axon beyond the axon initial segment 21,22 . One leading model posits that myosin Va specifically binds dendrite‐selective vesicles, but not axonal vesicles 19 .…”

Section: Introductionmentioning

confidence: 99%

A novel labeling strategy reveals that myosin Va and myosin Vb bind the same dendritically polarized vesicle population

Frank

Citarella

Quinones

et al. 2020

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Neurons are specialized cells with a polarized geometry and several distinct subdomains that require specific complements of proteins. Delivery of transmembrane proteins requires vesicle transport, which is mediated by molecular motor proteins. The myosin V family of motor proteins mediates transport to the barbed end of actin filaments, and little is known about the vesicles bound by myosin V in neurons. We developed a novel strategy to visualize myosin V‐labeled vesicles in cultured hippocampal neurons and systematically characterized the vesicle populations labeled by myosin Va and Vb. We find that both myosins bind vesicles that are polarized to the somatodendritic domain where they undergo bidirectional long‐range transport. A series of two‐color imaging experiments showed that myosin V specifically colocalized with two different vesicle populations: vesicles labeled with the transferrin receptor and vesicles labeled by low‐density lipoprotein receptor. Finally, coexpression with Kinesin‐3 family members found that myosin V binds vesicles concurrently with KIF13A or KIF13B, supporting the hypothesis that coregulation of kinesins and myosin V on vesicles is likely to play an important role in neuronal vesicle transport. We anticipate that this new assay will be applicable in a broad range of cell types to determine the function of myosin V motor proteins.

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Spatial control of membrane traffic in neuronal dendrites

Cited by 23 publications

References 324 publications

Cellular functions of actin- and microtubule-associated septins

Cellular functions of actin- and microtubule-associated septins

The Subcortical-Allocortical- Neocortical continuum for the Emergence and Morphological Heterogeneity of Pyramidal Neurons in the Human Brain

A novel labeling strategy reveals that myosin Va and myosin Vb bind the same dendritically polarized vesicle population

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