Spatial control of adult stem cell fate using nanotopographic cues

Ahn, Eun Hyun; Kim, Young-Hoon; Kshitiz, .; An, Steven S.; Afzal, Junaid; Lee, Suengwon; Kwak, Moon Kyu; Suh, Kahp Y.; Kim, Deok Ho; Levchenko, Andre

doi:10.1016/j.biomaterials.2013.11.037

Cited by 116 publications

(103 citation statements)

References 45 publications

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“…Surface topography alone [20] or in combination with substrate rigidity [21] have been shown to control mesenchymal stem cell lineage commitment. Recently, multi-scale patterned substrates have been shown to control adhesion and differentiation of human mesenchymal stem cells [22] and topographical features combined with hyaluronic acid have been shown to enhance chondrogenic differentiation of dental pulp stem cells [23].…”

Section: Introductionmentioning

confidence: 99%

Substrate topography: A valuable in vitro tool, but a clinical red herring for in vivo tenogenesis

et al. 2015

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractControlling the cell-substrate interactions at the bio-interface is becoming an inherent element in the design of implantable devices. Modulation of cellular adhesion in vitro, through topographical cues, is a well-documented process that offers control over subsequent cellular functions. However, it is still unclear whether surface topography can be translated into a clinically functional response in vivo at the tissue / device interface. Herein, we demonstrated that anisotropic substrates with a groove depth of ~317 nm and ~1,988 nm promoted human tenocyte alignment parallel to the underlying topography in vitro. However, the rigid poly(lactic-co-glycolic acid) substrates used in this study upregulated the expression of chondrogenic and osteogenic genes, indicating possible tenocyte trans-differentiation. Of significant importance is that none of the topographies assessed (~37 nm, ~317 nm and ~1,988 nm groove depth) induced extracellular matrix orientation parallel to the substrate orientation in a rat patellar tendon model. These data indicate that two-dimensional imprinting technologies are useful tools for in vitro cell phenotype maintenance, rather than for organised neotissue formation in vivo, should multifactorial approaches that consider both surface topography and substrate rigidity be established.

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Section: Introductionmentioning

confidence: 99%

Substrate topography: A valuable in vitro tool, but a clinical red herring for in vivo tenogenesis

et al. 2015

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“…The Young's modulus determined from AFM measurements is a relative value which is dependent on the experimental conditions, including environment (e.g., temperature, substrate, culture medium [72]), instrumental parameters (e.g., probe, loading rate, indentation depth [46]), cell (e.g., cellular positions being indented, cellular states [35]), data analysis (e.g., model selection, contact point determination [160]), and so on. Thus the results can be used for comparative studies only in cases when all experimental conditions are identical [77].…”

Section: Discussion and Perspectivementioning

confidence: 99%

“…The results by Nikkhah et al [76] showed that the reduction of serum in the culture medium can result in the decrease of cellular Young's modulus. This inspires us to consider the culture medium when investigating cell mechanics, especially for those cells require higher concentration of serum, such as stem cells [77]. Collectively, in order to make measurements comparable, conditions should be maintained identical during AFM cellular mechanical experiments.…”

Section: E Factors Influencing Afm Mechanical Measurementsmentioning

confidence: 99%

Atomic Force Microscopy in Characterizing Cell Mechanics for Biomedical Applications: A Review

Dang

Liu

et al. 2017

IEEE Trans.on Nanobioscience

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-Cell mechanics is a novel label-free biomarker for indicating cell states and pathological changes. The advent of atomic force microscopy (AFM) provides a powerful tool for quantifying the mechanical properties of single living cells in aqueous conditions. The wide use of AFM in characterizing cell mechanics in the past two decades has yielded remarkable novel insights in understanding the development and progression of certain diseases, such as cancer, showing the huge potential of cell mechanics for practical applications in the field of biomedicine. In this paper, we reviewed the utilization of AFM to characterize cell mechanics. First, the principle and method of AFM single-cell mechanical analysis was presented, along with the mechanical responses of cells to representative external stimuli measured by AFM. Next, the unique changes of cell mechanics in two types of physiological processes (stem cell differentiation, cancer metastasis) revealed by AFM were summarized. After that, the molecular mechanisms guiding cell mechanics were analyzed. Finally the challenges and future directions were discussed.

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“…[2,4] Microfluidic and nanotopographic patterning approaches establish some organ-level functions ex vivo, but lack the dimensionality to approximate developing tissues. [2,[12][13][14] Light is an ideal stimulus for perturbing the spatiotemporal dynamics of signals in living cells and organisms with high resolution. [15][16][17] The light-based optogenetic field has answered real biological questions [16] and developed tools for light-controlled genome editing and gene transfection.…”

Section: Doi: 101002/adma201603318mentioning

confidence: 99%