Spatial behavior and seizure-induced changes in c-fos mRNA expression in young and old rats

Abstract

The subcellular processes of gene induction and expression in the hippocampus are likely to underlie some of the known age-related impairments in spatial learning and memory. It is well established that immediate-early genes are rapidly and transiently induced in response to neuronal activity and this expression is required for stabilization of durable memories. To examine whether age-related memory impairment might be caused, in part, by differences in the level of cellular activation or subcellular processing, c-fos expression in CA1 pyramidal and dentate gyrus granule cells in the dorsal hippocampus of young and old rats was determined using fluorescence in situ hybridization and reverse transcription polymerase chain reaction. No significant age differences were found in the numbers of pyramidal or granule cells that show c-fos expression; however, cfos mRNA transcripts were altered in these 2 cell types in aged animals. These findings suggest that though the networks of cells that participate in behavior or seizure-induced activity are largely maintained in aged rats, their RNA transcript levels are altered. This might, in part, contribute to cognitive deficits frequently observed with advancing age.

Activity-dependent expression of miR-132 regulates immediate-early gene induction during olfactory learning in the greater short-nosed fruit bat, Cynopterus sphinx

“…We found that miR-132 expression paralleled with that of IEGs in the OB of C. sphinx at a similar time scale following training. It is important to note that the expression of IEGs is activity-dependent in neurons and leads to synaptic plasticity and memory formation (Chawla et al, 2013;Cheval et al, 2012;Ganesh et al, 2012;Pantazopoulos, Dolatshad, & Davis, 2011;Reinecke et al, 2013). Like IEGs, miR-132 expression is also induced rapidly by odor exposure (Nudelman et al, 2010;Pantazopoulos et al, 2011;Smalheiser et al, 2010) and facilitates synaptic plasticity (Hansen et al, 2010(Hansen et al, , 2013Scott et al, 2012;Tognini & Pizzorusso, 2012).…”

“…Studies in animal models have shown that behavioral training induces the expression of several protein kinases, including ERK-1/2 Duvarci, Nader, & Le Doux, 2005;Thomas & Huganir, 2004). ERK 1/2 phosphorylates the transcriptional activator cAMP response element binding protein-1 (CREB-1) at Ser 133 (Adams & Sweatt, 2002;Peng, Zhang, Zhang, Wang, & Ren, 2010;Thiels & Klann, 2001;Zhang, Okutani, Inoue, & Kaba, 2003), which in turn activates immediate-early genes (IEGs), including early growth response gene-1 (Egr-1) (Cheval et al, 2012;Ganesh, Bogdanowicz, Balamurugan, Ragu Varman, & Rajan, 2012;Guzowski, Setlow, Wagner, & McGaugh, 2001;Veyrac, Besnard, Caboche, Davis, & Laroche, 2014), C-fos (Charra, Datiche, Gigot, Schaal, & Coureaud, 2013;Chawla et al, 2013;Peng, Zhang, Ren, Zhang, & Wang, 2011), and C-jun (Reinecke, Herdegen, Eminel, Aldenhoff, & Schiffelholz, 2013). The expression of IEGs has been known to facilitates synaptic plasticity in the olfactory bulb (OB) (Brennan, Hancock, & Keverne, 1992;Charra et al, 2013;Ganesh et al, 2012;Guthrie, Anderson, Leon, & Gall, 1993;Veyrac, Wang, Baum, & Bakker, 2011).…”

Psychometric Properties of the Persian Version of the Quality of Life in Epilepsy Inventory in the Later Life

“…The aged samples replied to the 31 items of QOLIE-31. The QOLIE-31 questionnaire mainly consists of a 31-item disability/symptom scale regarding epilepsy that was investigated by authors and literature reviews 2,4,7,12,13,20,22,24,27,29. It was developed in three version i.e.…”

Role of Adenosine Signaling on Pentylenetetrazole-Induced Seizures in Zebrafish

“…25 To obtain detailed results about seizure manifestation in zebrafish, we analyzed the regulation of c-fos expression in the following experimental groups: vehicle (animals that were treated with vehicle and were not exposed to PTZ), vehicle + PTZ (animals that were exposed to PTZ without receiving pretreatment), and drugs + PTZ (animals that were pretreated with the different compounds studied and exposed to PTZ). All animals exposed to PTZ were tested at seizure stage III.…”