Spatial and temporal variability in the potential of river water biofilms to degrade p-nitrophenol

Kowalczyk, Agnieszka; Price, Oliver R.; Gast, Christopher J. van der; Finnegan, Christopher; Egmond, Roger A. van; Schäfer, Hendrik; Bending, Gary D.

doi:10.1016/j.chemosphere.2016.08.095

Cited by 5 publications

(2 citation statements)

References 58 publications

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“…Other studies have indicated that water flow [77] and oxygenation [78] contribute to the shaping of microbial communities in biofilms. Combined with temporal heterogeneity, geographic heterogeneity was observed with genera present in higher proportions at some sites than at others, in agreement with previous observations [79,80].…”

Section: Discussionsupporting

confidence: 91%

Do Microorganisms in Bathing Water in Guadeloupe (French West Indies) Have Resistance Genes?

Batantou Mabandza,

Colletin,

Dagot

et al. 2024

Antibiotics

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Waterborne faecal contamination is a major public health concern. The main objectives of this study were to investigate faecal contamination and Escherichia coli (E. coli) antibiotic resistance in recreational fresh water from Guadeloupe and to characterise the microbiome and resistome composition in biofilms from submerged rocks. Significant faecal contamination was observed at 14 freshwater sites. E. coli predominated (62%), followed by Enterobacter cloacae (11%) and Acinetobacter spp. (11%). Of 152 E. coli isolated, none produced extended-spectrum beta-lactamases (ESBLs), but 7% showed resistance to streptomycin and 4% to tetracycline. Biofilm resistome analysis revealed clinically significant antibiotic-resistance genes (ARGs), including those coding for resistance to sulfonamides (sul1), carbapenems (blaKPC), and third-generation cephalosporins (blaCTX-M). Mobile genetic elements (MGEs) (intI1, intI2, intI3) linked to resistance to aminoglycosides, beta-lactams, tetracycline, as well as heavy metal resistance determinants (copA, cusF, czcA, merA) conferring resistance to copper, silver, cadmium, and mercury were also detected. Diverse bacterial phyla were found in biofilm samples, of which Proteobacteria, Bacteroidetes, Planctonomycetes, and Cyanobacteria were predominant. Despite the frequent presence of E. coli exceeding regulatory standards, the low levels of antibiotic-resistant bacteria in freshwater and of ARGs and MGEs in associated biofilms suggest limited antibiotic resistance in Guadeloupean recreational waters.

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Section: Discussionsupporting

confidence: 91%

Do Microorganisms in Bathing Water in Guadeloupe (French West Indies) Have Resistance Genes?

Batantou Mabandza,

Colletin,

Dagot

et al. 2024

Antibiotics

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show abstract

“…For example, natural biofilms are capable of assimilating dissolved organic carbon originating from aquatic angiosperms in the overlying water, which results in a successful microbial community structure [23]. A similar biodegradable pattern of biofilms was also observed for synthetic organics such as p -nitrophenol [24]. Hence, distinct carbon source utilization by benthic biofilms may be used to interpret their metabolic functional characteristics and community structure to a certain degree.…”

Section: Introductionmentioning

confidence: 99%

Effects of Nanoplastics on Freshwater Biofilm Microbial Metabolic Functions as Determined by BIOLOG ECO Microplates

Miao

Guo

Liu

et al. 2019

IJERPH

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Nanoplastic (NP) contamination is becoming a pervasive issue as NPs, originating from microplastic particles, pose potentially harmful environmental impacts on aquatic ecosystems. The environmental hazards of NPs on microorganisms have been well documented in recent studies; however, little is known about their ecotoxicity effects on freshwater biofilms, which serve as important primary producers and decomposers and are highly connected with other ecosystem components. We investigated the effects of NPs on the microbial metabolic functions of freshwater biofilms in terms of carbon source utilization ability. Biofilm samples were collected, cultivated in a hydrodynamic flume for six weeks, and then exposed in polystyrene (PS) beads (100 nm in size) with different NP concentrations (1, 5, and 10 mg/L). BIOLOG ECO microplates were used to quantify carbon source utilization characteristics. The data were analyzed using average well-color development (AWCD), functional diversity indices, and principle component analysis (PCA). Results showed that the total carbon metabolic functions (represented by AWCD) remained constant (p > 0.05) with elevated NP concentrations, but some specific carbon sources (e.g., esters) changed in their utilization ability (p < 0.05). The microbial functional diversity (Shannon–Wiener diversity index, Simpson diversity index, and Shannon evenness index) was significantly reduced under 10 mg/L NPs (p < 0.05), indicating an inhibiting effect of NPs on biofilm metabolic diversity. This study examined NP ecotoxicity effects on microbial metabolic activities at the community level, but further studies are required to fully understand the mechanisms driving this change.

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Effects of point and nonpoint source pollution on urban rivers: From the perspective of pollutant composition and toxicity

Liu,

Su,

et al. 2023

Journal of Hazardous Materials

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Spatial and temporal variability in the potential of river water biofilms to degrade p-nitrophenol

Cited by 5 publications

References 58 publications

Do Microorganisms in Bathing Water in Guadeloupe (French West Indies) Have Resistance Genes?

Do Microorganisms in Bathing Water in Guadeloupe (French West Indies) Have Resistance Genes?

Effects of Nanoplastics on Freshwater Biofilm Microbial Metabolic Functions as Determined by BIOLOG ECO Microplates

Effects of point and nonpoint source pollution on urban rivers: From the perspective of pollutant composition and toxicity

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