Spatial and temporal expression of cytosine-5 DNA methyltransferase and DNA demethylase gene families of the Ricinus communis during seed development and drought stress

Genome-wide investigation and transcriptional analysis of cytosine-5 DNA methyltransferase and DNA demethylase gene families in tea plant (Camellia sinensis) under abiotic stress and withering processing

“…According to the result of putative subcellular localization, only CsDRM2b is located in the cytoplasm, whereas the other 11 proteins are localized in the nucleus. This result was consistent with previous findings that different RcDRM members are located in multiple organelles in R. communis (Victoria et al, 2018). The sequence similarity of these CsC5-MTase and CsdMTase proteins was also analyzed ( Fig.…”

“…Based on the above results, we concluded that the transcript abundance of CsC5-MTase and CsdMTase genes may be related to the stress degree of withered leaves. Similarly, previous work has demonstrated that C5-MTase and dMTase play important roles in response to salt stress (Liu et al, 2018b), cold stress (Gu et al, 2016), and drought stress (Victoria et al, 2018). Moreover, we observed a large number of light-responsive and stress-responsive cis-acting elements in the promoter regions of CsC5-MTase and CsdMTase.…”

“…With the extension of cold stress time, the transcript abundance of all CsC5-MTase genes showed a trend of continuous decline and reached the lowest point of transcript abundance at 48 h. In contrast, the transcript abundance of all CsdMTase genes showed a gradual increase under cold stress, reaching their highest transcript abundance at 48 h. In drought stress, the transcript abundance of CsC5-MTase and CsdMTase genes was similar under cold stress, and all CsC5-MTase genes also showed a descending trend under drought stress, while the transcript abundance of all CsdMTase genes increased significantly with increasing drought stress time. The results are consistent with the response of C5-MTase and dMTase to abiotic stress in R. communis (Victoria et al, 2018). These results indicated that these two gene families played different roles in the abiotic stress response.…”

“…In addition, DNA methylation also plays an important role in regulating growth and development Xing et al, 2015) and secondary metabolism (Bharti et al, 2015;Zhang et al, 2016). Since DNA methylation is involved in the regulation of a wide range of biological processes, C5-MTase and dMTase genes have been extensively identified and analyzed in several plant species, including A. thaliana (Ogneva, Dubrovina & Kiselev, 2016), Solanum lycopersicum (Cao et al, 2014, Arachis hypogaea (Wang et al, 2016b), Cynara cardunculus (Gianoglio et al, 2017) and Ricinus communis (Victoria et al, 2018). However, to the best of our knowledge, no investigator focused on the identification and analysis of C5-MTase and dMTase genes in tea plants based on genome-wide levels.…”

Azacitidine (5-AzaC)-treatment and mutations in DNA methylase genes affect embryogenic response and expression of the genes that are involved in somatic embryogenesis in Arabidopsis

“…The reprogramming of the cell transcriptome that results in the release of a new developmental programme is associated with extensive changes in the chromatin structure, which involves chemical modifications of DNA and histones (He et al 2011a;De-la-Peña et al 2015;Mozgová et al 2017). Among the epigenetic modifications that control gene expression, the methylation of DNA is considered to play a pivotal role in plant development (Zhang et al 2010;Turck and Coupland 2014;Victoria et al 2018).…”