Spatial and temporal distribution of cell wall polysaccharides during grain development of Brachypodium distachyon

Francin-Allami, Mathilde; Alvarado, Camille; Daniel, Sylviane; Geairon, Audrey; Guillon, Fabienne

doi:10.1016/j.plantsci.2018.12.018

Cited by 10 publications

(12 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is unusual because Brachypodium expresses the full set of genes required for starch synthesis in its endosperm [33]. Other aspects of grain development in Brachypodium have been reported [28,[33][34][35][36][37][38][39]. However, details of PCD events in developing Brachypodium grains, to the best of our knowledge, have not yet been reported.…”

Section: Introductionmentioning

confidence: 97%

Programmed Cell Death in Developing Brachypodium distachyon Grain

Saada

Solomon

Drea

2021

IJMS

View full text Add to dashboard Cite

The normal developmental sequence in a grass grain entails the death of several maternal and filial tissues in a genetically regulated process termed programmed cell death (PCD). The progression and molecular aspects of PCD in developing grains have been reported for domesticated species such as barley, rice, maize and wheat. Here, we report a detailed investigation of PCD in the developing grain of the wild model species Brachypodium distachyon. We detected PCD in developing Brachypodium grains using molecular and histological approaches. We also identified in Brachypodium the orthologs of protease genes known to contribute to grain PCD and surveyed their expression. We found that, similar to cereals, PCD in the Brachypodium nucellus occurs in a centrifugal pattern following anthesis. However, compared to cereals, the rate of post-mortem clearance in the Brachypodium nucellus is slower. However, compared to wheat and barley, mesocarp PCD in Brachypodium proceeds more rapidly in lateral cells. Remarkably, Brachypodium mesocarp PCD is not coordinated with endosperm development. Phylogenetic analysis suggests that barley and wheat possess more vacuolar processing enzymes that drive nucellar PCD compared to Brachypodium and rice. Our expression analysis highlighted putative grain-specific PCD proteases in Brachypodium. Combined with existing knowledge on grain PCD, our study suggests that the rate of nucellar PCD moderates grain size and that the pattern of mesocarp PCD influences grain shape.

show abstract

Section: Introductionmentioning

confidence: 97%

Programmed Cell Death in Developing Brachypodium distachyon Grain

Saada

Solomon

Drea

2021

IJMS

View full text Add to dashboard Cite

show abstract

“…Polysaccharides are also embedded into the cuticular layers [21]. Recently, xyloglucans were detected in the cuticle above the testa in the grain of the wild grass Brachypodium distachyon [22].…”

Section: Introductionmentioning

confidence: 99%

Cell Wall Proteome of Wheat Grain Endosperm and Outer Layers at Two Key Stages of Early Development

Cherkaoui

Lollier

Geairon

et al. 2019

IJMS

View full text Add to dashboard Cite

The cell wall is an important compartment in grain cells that fulfills both structural and functional roles. It has a dynamic structure that is constantly modified during development and in response to biotic and abiotic stresses. Non-structural cell wall proteins (CWPs) are key players in the remodeling of the cell wall during events that punctuate the plant life. Here, a subcellular and quantitative proteomic approach was carried out to identify CWPs possibly involved in changes in cell wall metabolism at two key stages of wheat grain development: the end of the cellularization step and the beginning of storage accumulation. Endosperm and outer layers of wheat grain were analyzed separately as they have different origins (maternal and seed) and functions in grains. Altogether, 734 proteins with predicted signal peptides were identified (CWPs). Functional annotation of CWPs pointed out a large number of proteins potentially involved in cell wall polysaccharide remodeling. In the grain outer layers, numerous proteins involved in cutin formation or lignin polymerization were found, while an unexpected abundance of proteins annotated as plant invertase/pectin methyl esterase inhibitors were identified in the endosperm. In addition, numerous CWPs were accumulating in the endosperm at the grain filling stage, thus revealing strong metabolic activities in the cell wall during endosperm cell differentiation, while protein accumulation was more intense at the earlier stage of development in outer layers. Altogether, our work gives important information on cell wall metabolism during early grain development in both parts of the grain, namely the endosperm and outer layers. The wheat cell wall proteome is the largest cell wall proteome of a monocot species found so far.

show abstract

“…New Phytologist parenchyma tissue can be found in the pith and in cortex pockets, which have been shown to function as carbon storage tissues during development (Jensen & Wilkerson, 2017). While this review focuses on secondary cell walls in Brachypodium distachyon and other grasses, wall synthesis has also been investigated using B. distachyon as a model system for callus tissue, young vegetative growth, and endosperm development (Christensen et al, 2010;Guillon et al, 2011;Liu et al, 2016;Betekhtin et al, 2018;Francin-Allami et al, 2019).…”

Section: Reviewmentioning

confidence: 99%

Grass secondary cell walls, Brachypodium distachyon as a model for discovery

2020

View full text Add to dashboard Cite

A key aspect of plant growth is the synthesis and deposition of cell walls. In specific tissues and cell types including xylem and fibre, a thick secondary wall comprised of cellulose, hemicellulose and lignin is deposited. Secondary cell walls provide a physical barrier that protects plants from pathogens, promotes tolerance to abiotic stresses and fortifies cells to withstand the forces associated with water transport and the physical weight of plant structures. Grasses have numerous cell wall features that are distinct from eudicots and other plants. Study of the model species Brachypodium distachyon as well as other grasses has revealed numerous features of the grass cell wall. These include the characterisation of xylosyl and arabinosyltransferases, a mixedlinkage glucan synthase and hydroxycinnamate acyltransferases. Perhaps the most fertile area for discovery has been the formation of lignins, including the identification of novel substrates and enzyme activities towards the synthesis of monolignols. Other enzymes function as polymerising agents or transferases that modify lignins and facilitate interactions with polysaccharides. The regulatory aspects of cell wall biosynthesis are largely overlapping with those of eudicots, but salient differences among species have been resolved that begin to identify the determinants that define grass cell walls.

show abstract

Spatial and temporal distribution of cell wall polysaccharides during grain development of Brachypodium distachyon

Cited by 10 publications

References 64 publications

Programmed Cell Death in Developing Brachypodium distachyon Grain

Programmed Cell Death in Developing Brachypodium distachyon Grain

Cell Wall Proteome of Wheat Grain Endosperm and Outer Layers at Two Key Stages of Early Development

Grass secondary cell walls, Brachypodium distachyon as a model for discovery

Contact Info

Product

Resources

About