Spatial and temporal changes in Actinobacterial dominance in experimental artificial groundwater recharge

Kolehmainen, Reija E.; Tiirola, Marja; Puhakka, Jaakko A.

doi:10.1016/j.watres.2008.07.039

Cited by 18 publications

(11 citation statements)

References 33 publications

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“…These taxa prefer living in water columns rather than interstitial spaces or attached to sediment53 which may explain their greater relative abundance in the open water of the well environment than in the aquifers. Given that most bacteria within pristine aquifers are attached rather than free-living5455, large abundances of Actinobacteria may be a useful post hoc indicator that samples were not drawn from the aquifer, and thus may not be representative of the communities within it.…”

Section: Discussionmentioning

confidence: 99%

Wells provide a distorted view of life in the aquifer: implications for sampling, monitoring and assessment of groundwater ecosystems

Korbel

Chariton

Stephenson

et al. 2017

Sci Rep

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When compared to surface ecosystems, groundwater sampling has unique constraints, including limited access to ecosystems through wells. In order to monitor groundwater, a detailed understanding of groundwater biota and what biological sampling of wells truly reflects, is paramount. This study aims to address this uncertainty, comparing the composition of biota in groundwater wells prior to and after purging, with samples collected prior to purging reflecting a potentially artificial environment and samples collected after purging representing the surrounding aquifer. This study uses DNA community profiling (metabarcoding) of 16S rDNA and 18S rDNA, combined with traditional stygofauna sampling methods, to characterise groundwater biota from four catchments within eastern Australia. Aquifer waters were dominated by Archaea and bacteria (e.g. Nitrosopumilales) that are often associated with nitrification processes, and contained a greater proportion of bacteria (e.g. Anaerolineales) associated with fermenting processes compared to well waters. In contrast, unpurged wells contained greater proportions of pathogenic bacteria and bacteria often associated with denitrification processes. In terms of eukaryotes, the abundances of copepods, syncarids and oligochaetes and total abundances of stygofauna were greater in wells than aquifers. These findings highlight the need to consider sampling requirements when completing groundwater ecology surveys.

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Section: Discussionmentioning

confidence: 99%

Wells provide a distorted view of life in the aquifer: implications for sampling, monitoring and assessment of groundwater ecosystems

Korbel

Chariton

Stephenson

et al. 2017

Sci Rep

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“…As in the studied case of the agro-industrial wastewater biotreatment, short-length PCR products (468-472 bp) dominated the mesophilic-activated sludge accounting for up to 40% of the calculated total peak area. A computer-assisted examination to assign the length variation of fragment sizes in different phylogenetic groups was conducted by Tiirola et al (2003) and Kolehmainen et al (2008), based on the cloning and sequencing analysis, suggesting that short rDNA templates (460-478 bp) are derived from members of alpha-proteobacteria. An explanation for the dominance of heterotrophic organisms as alpha-proteobacteria was attributed to the degradation of organic compounds during the wastewater treatment process, what is also in accordance with our results obtained for the molecular characterization of microbiota after phylogenetic affiliation from selected bands of TGGE profiles.…”

Section: Resultsmentioning

confidence: 99%

“…Temperature gradient gel electrophoresis (TGGE) and length heterogeneity-PCR (LH-PCR) fingerprinting methods are very reliable to monitor shifts in microbiota composition, its dynamics and synergies, and the individual member's relative contribution either in natural habitats or in man-modified environments like soil bioremediation and effluent biotreatment (e.g. Bernhard et al 2005;Díaz et al 2006;Doaré-Lebrun et al 2006;Henriques et al 2006;Kirk et al 2004;Kolehmainen et al 2008;Liu et al 2002;Moura et al 2009;Muyzer and Smalla 1998;Rincón et al 2006;Ritchie et al 2000;Sousa et al 2007;Talbot et al 2008;Tiirola et al 2003). Moreover, a great advantage of these methods is the possibility to evaluate and compare simultaneously a large number of multiple samples and in short time.…”

Section: Introductionmentioning

confidence: 99%

Molecular assessment of microbiota structure and dynamics along mixed olive oil and winery wastewaters biotreatment

et al. 2010

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The major parcel of the degradation occurring along wastewater biotreatments is performed either by the native microbiota or by added microbial inocula. The main aim of this study was to apply two fingerprinting methods, temperature gradient gel electrophoresis (TGGE) and length heterogeneity-PCR (LH-PCR) analysis of 16S rRNA gene fragments, in order to assess the microbiota structure and dynamics during mixed olive oil and winery wastewaters aerobic biotreatment performed in a jet-loop reactor (JLR). Sequence homology analysis showed the presence of bacterial genera Gluconacetobacter, Klebsiella, Lactobacillus, Novosphingobium, Pseudomonas, Prevotella, Ralstonia, Sphingobium and Sphingomonas affiliated with five main phylogenetic groups: alpha-, beta- and gamma-Proteobacteria, Firmicutes and Bacteroidetes. LH-PCR analysis distinguished eight predominant DNA fragments correlated with the samples showing highest performance (COD removal rates of 67 up to 75%). Cluster analysis of both TGGE and LH-PCR fingerprinting profiles established five main clusters, with similarity coefficients higher than 79% (TGGE) and 62% (LH-PCR), and related with hydraulic retention time, indicating that this was the main factor responsible for the shifts in the microbiota structure. Canonical correspondence analysis revealed that changes observed on temperature and O(2) level were also responsible for shifts in microbiota composition. Community level metabolic profile analysis was used to test metabolic activities in samples. Integrated data revealed that the microbiota structure corresponds to bacterial groups with high degradative potential and good suitability for this type of effluents biotreatments.

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“…dissolved organic carbon (DOC)/biodegradable dissolved organic carbon (BDOC)] of the water (Hendel et al, 2001;Kolehmainen et al, 2007Kolehmainen et al, , 2009. Moreover, the bacterial community changed from an Actinobacteria-dominanted population in lake water to a diverse and then primary proteobacterial community after travelling 0.6 m in the sand column (Kolehmainen et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Functional diversity changes of microbial communities along a soil aquifer for reclaimed water recharge

Zhang

Zhao

Zhang

2012

FEMS Microbiol Ecol

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The physiochemical and functional diversity of soil-attached microorganisms was investigated using a stabilized laboratory-scale soil aquifer treatment (SAT) system. In this system, reclaimed water after ozonation was used as the feed water, and 60% dissolved organic carbon was removed by the unsaturated vadose layer in 0.8 days. Soil biomass (volatile solids, phospholipid extraction) and functional diversity significantly decreased from the unsaturated vadose layer to the saturated aquifer, where they maintained the same level. Using principal components analysis based on substrate utilization pattern, the vadose layer soil sample was clearly separated from the saturated layer samples. Exceptionally, the oxidation rates of esters remained stable during SAT, indicating the purification potential on certain recalcitrant organic compounds in the saturated aquifer given an adequate retention time. Correlation analysis revealed that organic carbon was the key limiting factor for microbial biomass and activity, especially for tyrosine-like aromatic proteins and soluble microbial byproduct-like materials.

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Spatial and temporal changes in Actinobacterial dominance in experimental artificial groundwater recharge

Cited by 18 publications

References 33 publications

Wells provide a distorted view of life in the aquifer: implications for sampling, monitoring and assessment of groundwater ecosystems

Wells provide a distorted view of life in the aquifer: implications for sampling, monitoring and assessment of groundwater ecosystems

Molecular assessment of microbiota structure and dynamics along mixed olive oil and winery wastewaters biotreatment

Functional diversity changes of microbial communities along a soil aquifer for reclaimed water recharge

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