Spatial and Structural Metrics for Living Cells Inspired by Statistical Mechanics

Åberg, Christoffer; Varela, Juan A.; Fitzpatrick, Laurence W.; Dawson, Kenneth A.

doi:10.1038/srep34457

Cited by 11 publications

(20 citation statements)

References 32 publications

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“…Confocal microscopy imaging in different planes of the cells showed internalization of the PSNPs, and partial co-localization of the PSNPs with lysosomes after 24 h of exposure. Thereby, confirming previous studies indicating uptake of negatively charged 60 nm and 100 nm PSNPs [ 33 ], and 50 nm and 100 nm silica (SiO 2 ) NPs into the lysosomes [ 34 , 35 ] and localization of NPs in the cytoplasm around the nucleus (for 50 nm, 90 nm, and 100 nm PSNPs) [ 35 – 38 ].…”

Section: Discussionsupporting

confidence: 89%

Impact of nanoparticle surface functionalization on the protein corona and cellular adhesion, uptake and transport

et al. 2018

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BackgroundUpon ingestion, nanoparticles can interact with the intestinal epithelial barrier potentially resulting in systemic uptake of nanoparticles. Nanoparticle properties have been described to influence the protein corona formation and subsequent cellular adhesion, uptake and transport. Here, we aimed to study the effects of nanoparticle size and surface chemistry on the protein corona formation and subsequent cellular adhesion, uptake and transport. Caco-2 intestinal cells, were exposed to negatively charged polystyrene nanoparticles (PSNPs) (50 and 200 nm), functionalized with sulfone or carboxyl groups, at nine nominal concentrations (15–250 μg/ml) for 10 up to 120 min. The protein coronas were analysed by LC–MS/MS.ResultsSubtle differences in the protein composition of the two PSNPs with different surface chemistry were noted. High-content imaging analysis demonstrated that sulfone PSNPs were associated with the cells to a significantly higher extent than the other PSNPs. The apparent cellular adhesion and uptake of 200 nm PSNPs was not significantly increased compared to 50 nm PSNPs with the same surface charge and chemistry. Surface chemistry outweighs the impact of size on the observed PSNP cellular associations. Also transport of the sulfone PSNPs through the monolayer of cells was significantly higher than that of carboxyl PSNPs.ConclusionsThe results suggest that the composition of the protein corona and the PSNP surface chemistry influences cellular adhesion, uptake and monolayer transport, which might be predictive of the intestinal transport potency of NPs.Electronic supplementary materialThe online version of this article (10.1186/s12951-018-0394-6) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 89%

Impact of nanoparticle surface functionalization on the protein corona and cellular adhesion, uptake and transport

et al. 2018

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“…66 During this process, the vesicles are constantly trafficked toward the perinuclear space, increasing the distance to the cellular membrane and reducing the probability of fusing with it. 67 This means that there is a possibility of NP release in endosomes, but NP release becomes less probable after trafficking into lysosomes or phagolysosomes.…”

Section: Resultsmentioning

confidence: 99%

Reduction of Nanoparticle Load in Cells by Mitosis but Not Exocytosis

et al. 2019

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The long-term fate of biomedically relevant nanoparticles (NPs) at the single cell level after uptake is not fully understood yet. We report that lysosomal exocytosis of NPs is not a mechanism to reduce the particle load. Biopersistent NPs such as nonporous silica and gold remain in cells for a prolonged time. The only reduction of the intracellular NP number is observed via cell division, e.g., mitosis. Additionally, NP distribution after cell division is observed to be asymmetrical, likely due to the inhomogeneous location and distribution of the NP-loaded intracellular vesicles in the mother cells. These findings are important for biomedical and hazard studies as the NP load per cell can vary significantly. Furthermore, we highlight the possibility of biopersistent NP accumulation over time within the mononuclear phagocyte system.

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“…35 Another study combined the experimental observation of NP uptake with correlation statistical analysis to predict the effect of NP internalization on cellular organelle distribution inside cells. 36 In the work presented here, we aim to study the influence of the molecular characteristics of different ligands on gold (Au) NPs in vitro, evaluating the state of aggregation in vitro and during intracellular trafficking. Our aim is to understand whether or not the properties, conferred by the ligands, are retained by the NPs following uptake.…”

Section: Introductionmentioning

confidence: 99%

Influence of surface coating on the intracellular behaviour of gold nanoparticles: a fluorescence correlation spectroscopy study

et al. 2017

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In the biomedical applications of nanoparticles (NPs), the proper choice of surface chemistry is a crucial aspect in their design. The nature of the coating can heavily impact the interaction of NPs with biomolecules, affect the state of aggregation, and ultimately determine their biological fate. As such, protein corona formation and the aggregation behaviour of gold NPs (Au NPs) are studied here. Au NPs are prepared with four distinct surface functionalisations, namely mercaptosuccinic acid (MSA), N-4-thiobutyroil glucosamine, HS-PEG and HS-alkyl-PEG. Corona formation, aggregation, and the intracellular behaviour of the Au NPs are then investigated by means of Fluorescence Correlation Spectroscopy (FCS) in cell culture media and in live cells. To evaluate the state of aggregation and the formation of a protein corona, the Au NPs are incubated in cell media and the diffusion coefficient is determined via FCS. The in vitro behaviour is compared with the level of aggregation of the NPs in cells. Diffusion times of the NPs are estimated at different positions in the cell after a one hour incubation period. It is found that the majority of MSA and glucose-Au NPs are present inside the cell as slowly diffusing species with diffusion times (τ) greater than 6000 μs (hydrodynamic diameter >250 nm). PEGylated Au NPs adsorb a small amount of protein and manifest low agglomeration both in media and in living cells. In particular, the HS-alkyl-PEG coating shows an excellent correlation between lower protein adsorption, 4-fold lower compared to the MSA coated NPs, and limited intracellular aggregation. In the case of single HS-alkyl-PEG coated NPs, it is found that typical intracellular τ values range from 500 to 1500 μs, indicating that these particles display reduced aggregation in the intracellular environment.

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Spatial and Structural Metrics for Living Cells Inspired by Statistical Mechanics

Cited by 11 publications

References 32 publications

Impact of nanoparticle surface functionalization on the protein corona and cellular adhesion, uptake and transport

Impact of nanoparticle surface functionalization on the protein corona and cellular adhesion, uptake and transport

Reduction of Nanoparticle Load in Cells by Mitosis but Not Exocytosis

Influence of surface coating on the intracellular behaviour of gold nanoparticles: a fluorescence correlation spectroscopy study

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