Spatial and Functional Relationship of GGAs and AP‐1 in <i>Drosophila</i> and HeLa Cells

Hirst, Jennifer; Sahlender, Daniela A.; Choma, Maja K.; Sinka, Rita; Harbour, Michael E.; Parkinson, Michael; Robinson, Margaret S.

doi:10.1111/j.1600-0854.2009.00983.x

Cited by 57 publications

(82 citation statements)

References 49 publications

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“…Depletion of γ1 led to the increased secretion of pro-catD into the medium and a consequent reduction in the amount of intracellular m-catD ( Fig. S3), indicating a partial impairment of transport to lysosomes, as previously observed by Hirst et al, (2009). In γ2 KD cells, the ratio of intracellular m-catD and secreted pro-catD was similar to in control cells (Fig.…”

Section: Resultssupporting

confidence: 64%

CD4 downregulation by the HIV-1 protein Nef reveals distinct roles for the γ1 and γ2 subunits of the AP-1 complex in protein trafficking

Tavares

Silva

Silva-Januário

et al. 2017

Journal of Cell Science

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The HIV accessory protein Nef is a major determinant of viral pathogenesis that facilitates viral particle release, prevents viral antigen presentation and increases infectivity of new virus particles. These functions of Nef involve its ability to remove specific host proteins from the surface of infected cells, including the CD4 receptor. Nef binds to the adaptor protein 2 (AP-2) and CD4 in clathrin-coated pits, forcing CD4 internalization and its subsequent targeting to lysosomes. Herein, we report that this lysosomal targeting requires a variant of AP-1 containing isoform 2 of γ-adaptin (AP1G2, hereafter γ2). Depletion of the γ2 or μ1A (AP1M1) subunits of AP-1, but not of γ1 (AP1G1), precludes Nef-mediated lysosomal degradation of CD4. In γ2-depleted cells, CD4 internalized by Nef accumulates in early endosomes and this alleviates CD4 removal from the cell surface. Depletion of γ2 also hinders EGFR-EGF-complex targeting to lysosomes, an effect that is not observed upon γ1 depletion. Taken together, our data provide evidence that the presence of γ1 or γ2 subunits delineates two distinct variants of AP-1 complexes, with different functions in protein sorting.

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Section: Resultssupporting

confidence: 64%

CD4 downregulation by the HIV-1 protein Nef reveals distinct roles for the γ1 and γ2 subunits of the AP-1 complex in protein trafficking

Tavares

Silva

Silva-Januário

et al. 2017

Journal of Cell Science

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“…2B). AP-1 is a TGN marker 36 and in control salivary cells localizes to structures adjacent to, but distinct from, Lva-marked Golgi bodies (Fig. 2B).…”

Section: Resultsmentioning

confidence: 99%

The Garz Sec7 domain guanine nucleotide exchange factor for Arf regulates salivary gland development in Drosophila

Szul¹,

Burgess

Jeon

et al. 2011

Cellular Logistics

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“…The differences we describe here cannot be ascribed to cell type specificities as we obtained the same results in HeLaM and NRK cells. In a more recent study of GGA and AP-1 localization at both the light and EM level in Drosophila Dmel2 and HeLa cells, Hirst et al (71) described cargo-dependent recruitment and discovered that AP-1 and GGA staining are in close apposition, although they are not superimposable. The authors point out the difficulties in clearly discriminating between Golgi and endosomal staining and acknowledge that both AP-1 and GGA staining is equally likely to be endosomal.…”

Section: Gga1mentioning

confidence: 99%

A Role for Cargo in Arf-dependent Adaptor Recruitment

Caster

Sztul

Kahn

2013

Journal of Biological Chemistry

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Background: Cargo proteins recruit Arf-dependent adaptors for packaging. Results: Cargo presence at the Golgi/endosomes leads to specific adaptor recruitment. Conclusion: Arf activation is more closely coupled to cargo than previously appreciated, and we interpret the specificity in adaptor recruitment as evidence of the lack of freely diffusible activated Arfs. Significance: Cargos play a role in the activation of Arfs and recruitment of specific adaptors.

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Spatial and Functional Relationship of GGAs and AP‐1 in Drosophila and HeLa Cells

Cited by 57 publications

References 49 publications

CD4 downregulation by the HIV-1 protein Nef reveals distinct roles for the γ1 and γ2 subunits of the AP-1 complex in protein trafficking

CD4 downregulation by the HIV-1 protein Nef reveals distinct roles for the γ1 and γ2 subunits of the AP-1 complex in protein trafficking

The Garz Sec7 domain guanine nucleotide exchange factor for Arf regulates salivary gland development in Drosophila

A Role for Cargo in Arf-dependent Adaptor Recruitment

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