SPARC‐like 1 (SC1) is a diversely expressed and developmentally regulated matricellular protein that does not compensate for the absence of SPARC in the CNS

Abstract

SPARC-like 1 (SC1) is a member of the SPARC family of matricellular proteins that has been implicated in the regulation of processes such as cell migration, proliferation, and differentiation. Here we show that SC1 exhibits remarkably diverse and dynamic expression in the developing and adult nervous system. During development, SC1 localizes to radial glia and pial-derived structures, including the vasculature, choroid plexus, and pial membranes. SC1 is not downregulated in postnatal development, but its expression shifts to distinct time windows in subtypes of glia and neurons, including astrocytes, large projection neurons, Bergmann glia, Schwann cells, and ganglionic satellite cells. In addition, SC1 expression levels and patterns are not altered in the SPARC null mouse, suggesting that SC1 does not compensate for the absence of SPARC. We conclude that SC1 and SPARC may share significant homology, but are likely to have distinct but complementary roles in nervous system development.

Cortical hypoplasia and ventriculomegaly of p73‐deficient mice: Developmental and adult analysis

“…Rabbit polyclonal anti‐S100 is a cell‐type marker for glial cells. Immunostaining of mouse brain sections produces a specific staining pattern of S100‐positive glia identical to that published previously (Lloyd‐Burton and Roskams, ). Rabbit polyclonal Tbr1 antibody recognizes T‐box brain 1, a transcription factor that is expressed in glutamatergic neurons during cortical development (Mendez‐Gomez et al, ; Lloyd‐Burton and Roskams, ).…”

Brain endothelial cell expression of SPARCL‐1 is specific to chronic multiple sclerosis lesions and is regulated by inflammatory mediators in vitro

“…Expression of SPARCL‐1 by astrocytes, and to a lesser extent neurons, is well‐characterized in healthy CNS, during development and following acute injury , but its expression by BEC has not been reported before. Here, we show that SPARCL‐1 expression by BEC is restricted to chronic active and inactive MS lesions, suggesting a stage‐specific role of SPARCL‐1 in MS.…”

Germline Chd8 haploinsufficiency alters brain development in mouse

“…Slides were incubated for 24 h at 4 °C in a blocking solution containing normal donkey serum (5% v/v) diluted in PBS-T (1× phosphate buffered saline and 0.01% (v/v) Triton X-100), rinsed in PBS-T, and incubated for 24 h at 4°C in primary antibody solution containing anti-Ctip2 44 (ab18465; Abcam), anti-Tbr1 44 (ab31940; Abcam) and anti-Brn2 45 (sc-6029; Santa Cruz Biotechnology) antibodies, each diluted 1:500 in PBS-T. These antibodies have been previously used for IHC analysis in brain 46,47 . The slides were then rinsed in PBS-T and incubated overnight at 4°C in fluorophore-conjugated secondary antibodies (711-545-152 and 712-165-153; Jackson ImmunoResearch).…”